Sertoli cell-mediated differentiation of bovine fetal mesenchymal stem cells into germ cell lineage using an in vitro co-culture system

Segunda, Moisés N.; Bahamonde, Javiera; Muñoz, I.; Sepúlveda, Soledad; Cortez, Jahaira; Reyes, M. De los; Palomino, Jaime; Torres, CG; Peralta, Oscar A.

doi:10.1016/j.theriogenology.2019.02.034

Cited by 13 publications

(19 citation statements)

References 37 publications

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“…Prostaglandin D synthase was also reported by Valencia et al [52] as a seminal plasma protein related to fertility and is located on the 6 th chromosome. The role of this protein is to bind, protect, and facilitate the absorption of retinoids in male genital organs, which are needed in the process of spermatogenesis [53]. Intra et al [54] also reported α-L-fucose as a protein that plays a role in fertility, especially in binding/sperm penetration in the pellucid zone, and the fusion of the sperm-oocyte membrane.…”

Section: Plasma Seminal Proteinsmentioning

confidence: 99%

Protamine and other proteins in sperm and seminal plasma as molecular markers of bull fertility

2020

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Fertility is the most important aspect in the efforts to increase livestock populations. Protamine and various proteins in sperm and seminal plasma are the results of the molecular analysis which can be used as a marker of fertility. Each of the proteins plays an important role in the normal function of sperm, starting from the formation of sperm structure, motility, capacitation, cell protection, acrosome reactions, successful fertilization, egg activation, and embryonic development. Finally, these molecular components can be a marker of fertility and can help to diagnose the cases of infertility/subfertility in livestock in the field.

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Section: Plasma Seminal Proteinsmentioning

confidence: 99%

Protamine and other proteins in sperm and seminal plasma as molecular markers of bull fertility

2020

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“…Subsequently, WT1 (Wilms' tumor protein), a widely used specific marker of mature Sertoli cells (Segunda et al 2019), was selected to analyze if CREST is selectively expressed in the mature Sertoli cells. The adjacent section ABC double staining on 2-µm-thick serial sections of the adult rat testis showed that CREST was colocalized with WT1 in the Sertoli cells of the adult rat testis (Fig.…”

Section: Crest Is Highly and Exclusively Expressed In The Mature Sertoli Cells Of Adult Testismentioning

confidence: 99%

“…To see if CREST might play a role in development of Sertoli cells as it does in development of neurons (Aizawa et al 2004;Staahl et al 2013), the expression pattern of CREST in the rat testis at different postnatal stages (P1, P5, P7, P9, P14, P22, P34, 6 month) was examined using double immunofluorescent labeling in which GATA-4 was used as a marker for immature Sertoli cells while WT1 as a marker for mature Sertoli cells (Viger et al 1998;Walker 2003;Segunda et al 2019). At early postnatal stages (P1-P14), CREST immunofluorescent intensity in GATA-4 + Sertoli cells was strong, and gradually increasing with developmental stages.…”

Section: Expression Of Crest In the Sertoli Cells Increases With Postnatal Development Of The Ratsmentioning

confidence: 99%

Protein expression pattern of calcium-responsive transactivator in early postnatal and adult testes

Jiao

et al. 2021

Histochem Cell Biol

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Calcium-responsive transactivator (CREST), a nuclear protein highly expressed in postmitotic neurons, is involved in the regulation of cell cycle, differentiation and dendritic development of neuronal cells. Its mRNA has been detected in the testis of adult rat, whilst its protein expression and distribution pattern in the testis remain to be elucidated. In this study, we examined the distribution of CREST in the adult testes of both rats and human as well as the expression pattern of CREST in the testes of postnatal developing rats. In the adult testes of both human and rats, immunohistochemical analysis revealed that CREST was selectively distributed in the mature Sertoli cells but not in the spermatogenic cells. In the testes of postnatal developmental rats, CREST was expressed not only in Sertoli cells but also in the gonocytes and spermatogenic cells at the initial stage of spermatogenic cell differentiation. CREST immunoreactivity continued to increase in Sertoli cells during differentiation, reaching its peak in adulthood. However, CREST immunostaining intensity dramatically decreased as the spermatogenic cells differentiate, disappearing in the post-differentiation stage. Furthermore, Brg1 and p300, two CREST-interacting proteins ubiquitously expressed in the body, are found to be colocalized with CREST in the spermatogenic epithelial cells including Sertoli cells. The unique expression pattern of CREST in developing testis suggests that CREST might play regulatory roles in the differentiation of spermatogenic epithelial cells. The Sertoli cell-specific expression of CREST in the adulthood hints that CREST might be a novel biomarker for the mature Sertoli cells.

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“…However, results were only partially positive since no expression was detected for VASA, STELLA, FRAGILIS, STRA8 or PIWIL2. A recent study has shown that co‐culture with Sertoli cells from bull's testis might play an important role in the differentiation of bovine MSCs into germ cells (Segunda et al, ), upregulating the expression of DAZL, PIWIL2 and SCP3 compared to monocultures. In goats, male PGCLCs have been obtained by transfection of STRA8, BOULE and DAZL into bone MSCs (Zhang et al, ).…”

Section: In Vitro Gametogenesis From Other Adult Stem Cellsmentioning

confidence: 99%

Gametes from stem cells: Status and applications in animal reproduction

Goszczynski

Denicol

Ross

2019

Reprod Domestic Animals

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In vitro gamete differentiation could revolutionize animal production by decreasing generation intervals, increasing the number of gametes per animal and facilitating the dissemination of elite genetics. In addition, it could help to develop new strategies for the conservation of endangered species. The recent in vitro reconstitution of germ cell development in mice has inspired researchers to invest their best efforts into reproducing this achievement in livestock species. With this goal in mind, multiple differentiation approaches and cell sources have been evaluated. The degree of success in these evaluations varies according to the species and the stage of development studied, but, in general, partially positive results have been obtained. Evidence suggests that although functional gametes with true reproductive potential are still to be obtained, it is a matter of time before this goal is achieved. K E Y W O R D S embryo, gametogenesis, stem cells | 23 GOSZCZYNSKI et al.

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Sertoli cell-mediated differentiation of bovine fetal mesenchymal stem cells into germ cell lineage using an in vitro co-culture system

Cited by 13 publications

References 37 publications

Protamine and other proteins in sperm and seminal plasma as molecular markers of bull fertility

Protamine and other proteins in sperm and seminal plasma as molecular markers of bull fertility

Protein expression pattern of calcium-responsive transactivator in early postnatal and adult testes

Gametes from stem cells: Status and applications in animal reproduction

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