Serum adenosine deaminase in patients with rheumatoid arthritis treated with methotrexate

Salesi, Mansour; Ghazvini, Rozita Aghaye; Farajzadegan, Ziba; Karimifar, Mansoor; Karimzadeh, Hadi; Masoumi, Maryam; Ebrahimi, Bahareh

doi:10.4103/2279-042x.108374

Cited by 13 publications

(17 citation statements)

References 14 publications

(26 reference statements)

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“…In our previous study, we found inverse correlation between serum ADA level and ESR during acute phase of JIA and between serum ADA level and WBC counts after disease control (25). Salesi et al reported significant correlation between serum ADA and ESR (15), but the result of our recent study was compatible with that of Sari et al (19). Vinapamula et al found significant positive correlation between serum ADA and CRP in RA patients.…”

Section: Discussionsupporting

confidence: 81%

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Comparison of Adenosine Deaminase Level in Serum and Synovial Fluid in Patients with Juvenile Idiopathic Arthritis and Its Relation to Inflammatory Acute Phase Reactants

Rahmani¹,

Raeeskarami²,

Ziaee³

et al. 2017

Iran J Pediatr

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Background: In JIA, cell-mediated immune response results in secretion of different inflammatory products from activated lymphocytes, macrophages, fibroblasts and leukocytes in synovial joints. Adenosine deaminase (ADA) regulates this immune system activity by metabolizing adenosine through purine metabolic pathway. Objectives: The aim of this study was to compare the level of serum ADA with synovial fluid ADA in JIA patients and to see whether it can be utilized as a marker for the activity of the disease Methods: JIA was diagnosed based on International League of Associations for Rheumatology diagnosis criteria. ADA was measured using special kits. Results: 80% of the patients had oligoarticular and 20% polarticulare JIA. There was a significant relation between erythrocyte sedimentation rate (ESR) and high level of synovial fluid ADA. Synovial ADA level was significantly higher than serum ADA in polyarticular JIA. C-reactive protein (CRP), leukocytes and platelets count were increased in high levels of synovial fluid ADA. No correlation was observed between level of serum and synovial fluid ADA. Conclusions: Synovial fluid ADA seems a more precise index than serum ADA to assess the inflammatory condition. In addition, acute-phase response reactants such as ESR, CRP, and platelets count could be suitable predicting parameters for arthritis.

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Section: Discussionsupporting

confidence: 81%

“…Adenosine deaminase (ADA) is one of the main enzymes of purine metabolic pathway, and is responsible to metabolize adenosine. ADA represents an exclusive checkpoint in the extracellular adenosine regulation and, consequently, in the control of immune system activity through its modulation of adenosine pathways (15)(16)(17)(18)(19)(20).…”

Section: Introductionmentioning

confidence: 99%

Comparison of Adenosine Deaminase Level in Serum and Synovial Fluid in Patients with Juvenile Idiopathic Arthritis and Its Relation to Inflammatory Acute Phase Reactants

Rahmani¹,

Raeeskarami²,

Ziaee³

et al. 2017

Iran J Pediatr

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“…Serum ADA levels in patient and control groups were determined and the relation between ADA level and disease activity parameters were evaluated. Although the exact effect of MTX on the ADA enzymatic activity is not clearly understood, it is assumed that in purine enzyme metabolic pathway, MTX increases adenosine and inhibits ADA both directly and indirectly [ 10 ]. Therefore the RA group was divided into two groups as patients receiving MTX and patients on drugs other than MTX.…”

Section: Methodsmentioning

confidence: 99%

“…There are conflicting reports about the relationship of ADA and disease activity parameters in the English literature [ 5 - 16 ]. Serum ADA was reported to be increased in serum and synovial fluid of RA patients compared with non-inflammatory arthritic conditions [ 7 , 10 , 11 ]. ADA has been suggested as a prediction marker or a useful biochemical marker of inflammatory process in patients with RA [ 13 , 14 ], while some studies did not find a correlation between ADA levels and disease activity markers [ 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

Serum Adenosine Deaminase Level is High But Not Related with Disease Activity Parameters in Patients with Rheumatoid Arthritis

Demir¹,

Borman²,

Ayhan³

et al. 2014

TORJ

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Serum adenosine deaminase (ADA) has been previously proposed to predict disease activity in patients with rheumatoid arthritis (RA). The aim of this study was to investigate the level of serum ADA, and the relationship between ADA and disease activity markers, in a group of patients with RA.A hundred and 10 patients with a diagnosis of RA were recruited from outpatient clinic of Rheumatology Unit. Demographic properties comprising age, gender, disease duration and drugs were recorded. Disease activity based on disease activity score (DAS)28-erythrocyte sedimentation rate (ESR) and DAS28- C reactive protein (CRP,) ESR, CRP levels, as well as pain by visual analog scale and rheumatoid factor (RF) were recorded. Serum ADA levels (IU/L) were determined in all RA patients and in 55 age and sex similar healthy control subjects.Ninety-six female and 14 male RA patients with a mean age of 54.32±11.51, and with a mean disease duration of 11.5±9.13 years were included to the study. The control group comprised of 48 female and 7 male healthy subjects. 35.5% of the patients were on methotrexate (MTX) and 64.5% of patients were on combined DMARDs or combined MTX and anti-TNF therapies. The mean serum ADA level was statistically higher in RA patients than in control subjects (27.01±10.6 IU/L vs 21.8 ±9.9 IU/L). The mean values of ESR (23.2±14.8 mm/h), CRP (1.71±1.11mg/dL), pain by VAS (37.2±27.1), DAS28-ESR (2.72±0.77), DAS28 CRP (1.37±0.5) were not correlated with ADA levels (p>0.05).Our results have shown that serum ADA levels are higher in RA patients than in controls but were not related with any of the disease activity markers. We conclude that ADA in the serum may not be a reliable biochemical marker to predict disease activity in patients with RA.

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“…Interestingly, the anti-inflammatory effects of EA seem to be similar to the effects of methotrexate. Both treatments result in an increase in the extracellular concentration of adenosine and the downregulation of ADA [ 25 – 27 ].…”

Section: Discussionmentioning

confidence: 99%

Repeated Electroacupuncture Persistently Elevates Adenosine and Ameliorates Collagen‐Induced Arthritis in Rats

Tian-shen

Li³

et al. 2016

Evidence-Based Complementary and Alternative Medicine

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The aim of this paper was to investigate the effect of repeated electroacupuncture (EA) over 21 days on the adenosine concentration in peripheral blood of rats with collagen-induced arthritis (CIA). Wistar rats were divided into three groups of 6 animals each: sham-control, CIA-control, and CIA-EA. We determined the adenosine concentration in peripheral blood and assessed pathological changes of ankle joints. Quantitative reverse-transcription-polymerase chain reaction was used to determine mRNA levels of ecto-5′-nucleotidase (CD73), adenosine deaminase (ADA), and tumor necrosis factor-alpha (TNF-α). Immunohistochemical staining was used to detect expression of ADA and CD73 in synovial tissue. Repeated EA treatment on CIA resulted in the persistence of high concentrations of adenosine in peripheral blood, significantly reduced pathological scores, TNF-α mRNA concentrations, and synovial hyperplasia. Importantly, EA treatment led to a significant increase in CD73 mRNA levels in peripheral blood but was associated with a decrease of CD73 immunostaining in synovial tissue. In addition, EA treatment resulted in a significant decrease of both ADA mRNA levels in peripheral blood and ADA immunostaining in synovial tissue. Thus, repeated EA treatment exerts an anti-inflammatory and immunoregulatory effect on CIA by increasing the concentration of adenosine. The mechanism of EA action may involve the modulation of CD73 and ADA expression levels.

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Serum adenosine deaminase in patients with rheumatoid arthritis treated with methotrexate

Cited by 13 publications

References 14 publications

Comparison of Adenosine Deaminase Level in Serum and Synovial Fluid in Patients with Juvenile Idiopathic Arthritis and Its Relation to Inflammatory Acute Phase Reactants

Comparison of Adenosine Deaminase Level in Serum and Synovial Fluid in Patients with Juvenile Idiopathic Arthritis and Its Relation to Inflammatory Acute Phase Reactants

Serum Adenosine Deaminase Level is High But Not Related with Disease Activity Parameters in Patients with Rheumatoid Arthritis

Repeated Electroacupuncture Persistently Elevates Adenosine and Ameliorates Collagen‐Induced Arthritis in Rats

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