Serum enzymatic changes modulated using trypsin: chymotrypsin preparation during burn wounds in humans

Latha, B.; Ramakrishnan, Mathangi; Jayaraman, V.; Babu, Mary

doi:10.1016/s0305-4179(97)00080-6

Cited by 17 publications

(9 citation statements)

References 15 publications

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“…The precise etiology of these changes is not known, but they usually are benign in character and resolve spontaneously. Liver ALP activity was determined in the present study and it increased significantly in burn group consistent with other burn studies (1,36). Both silymarin treatments did not reduce this increase.…”

Section: Discussionsupporting

confidence: 91%

The effect of silymarin on the liver in thermal burn injury

Yemişen¹

2014

mpj

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Section: Discussionsupporting

confidence: 91%

The effect of silymarin on the liver in thermal burn injury

Yemişen¹

2014

mpj

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“…This trend in the values shows that the transaminases rise transiently in response to the thermal injury and they shortly fall back to original level. These changes in transaminases and ALP confirm the earlier reports (11,12).…”

Section: Serum Enzymes In Thermal Injurysupporting

confidence: 92%

“…Various metabolic and biochemical alterations that follow burn injury include increased free radical activity (7)(8)(9), bloods levels of ascorbic acid, uric acid (9) and elevation of the activity of some serum enzymes (10)(11)(12)(13). Changes in the serum transaminases (AST & ALT) and alkaline phosphatase (ALP), creatinine kinase, lactate dehydrogenase, J-glutamyl transpeptidase have been reported in burn patients after not different from the controls ( Table-1), whereas amylase and TBARS were significantly (p<0.001) elevated in the patients as compared to the controls on the day of admission.…”

Section: Introductionmentioning

confidence: 99%

Serum enzymes in thermal injury

Bhagwat

Subrahmanyam

Pujari

2007

Indian J Clin Biochem

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“…Chymotrypsin dissolves soft fibrin and cleans the proteinecious debris at inflammation sites (Swamy and Patil, 2008). Latha et al (1997) used a trpsinchymotrypsin combination in the ratio of 6:1 as an antiinflammation agent to treat burn patients and found superior results compared to the use of trypsin alone.…”

Section: Anti-inflammation and Prevention Of Wound Infectionmentioning

confidence: 99%

Extraction, Purification and Characterization of Fish Chymotrypsin: A Review

K.¹

2011

American Journal of Biochemistry and Biotechnology

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Problem statement: Solid fish waste is generated from the unwanted parts of fish including heads, tails, fins, frames, offal (guts, kidney and liver) and skin. It accounts for up to 80% of material from production of surimi, 66% from production of fillet and 27% from production of headed and gutted fish. Currently, fish wastes are disposed off in land-based waste disposal systems or at sea generating toxic byproducts during the decomposition process. However, fish processing waste can be used to produce commercially valuable by-products, such as chymotrypsin. Approach: A comperehensive review of the literature on the extraction, purification and characeterization of fish chymotrypsin was performed. Results: Chymotrypsin is an endopeptidase secreted by the pancreatic tissues of vertebrates and invertebrates. It has 3 different structures (chymotrypsin A, B and C) varying slightly in solubility, electrophoretic mobility, isoelectric point and cleavage specificity. Only chymotrypsin A and B are found in fish. Compared with mammal chymotrypsin, fish chymotrypsins have similar amino acid composition and molecular weights. Fish chymotrypsins have higher specific activity, especially those from cold-water fish, and low pH and temperature tolerance. The factors affecting the concentration and activity of chymotrypsin in fish are water temperature, fish species, fish age, fish weight and starvation. Chymotrypsin has application in various industries including the food industry, leather production industry chemical industry and medical industry. Conclusion: Extraction techniques for chymotrypsin include: ultra-filtration, ammonium sulphate fractionation precipitation or water-in-oil microemulsions. Purification can be carried out using recrystallization and gel-filtration, ion-exchange and hydrophobic interaction chromatography. Further studies should focus on the optimization of purifiying chymotrypsin from fish processing wastes.

show abstract

Serum enzymatic changes modulated using trypsin: chymotrypsin preparation during burn wounds in humans

Cited by 17 publications

References 15 publications

The effect of silymarin on the liver in thermal burn injury

The effect of silymarin on the liver in thermal burn injury

Serum enzymes in thermal injury

Extraction, Purification and Characterization of Fish Chymotrypsin: A Review

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