Serum glycated albumin as a new glycemic marker in pediatric diabetes

Abstract

PurposeSerum glycated albumin (GA) has been recently used as another glycemic marker that reflects shorter term glycemic control than glycated hemoglobin (HbA1c). Insulin secretory function and glycemic fluctuation might be correlated with the ratio of GA to HbA1c (GA/HbA1c) in diabetic adult patients. This study investigated the association of GA and GA/HbA1c ratio with the levels of fasting C-peptide, fasting plasma glucose in type 1 and type 2 pediatric diabetes.MethodsTotal 50 cases from 42 patients were included. The subjects were classified into type 1 diabetes mellitus (T1DM) (n=30) and type 2 diabetes mellitus (T2DM) (n=20) group. The associations among HbA1c, GA, and GA/HbA1c ratio were examined. The relationship between the three glycemic indices and fasting glucose, fasting C-peptide were analyzed.ResultsMean values of GA, the GA/HbA1c ratio were significantly higher in T1DM than T2DM. GA (r=0.532, P=0.001), HbA1c (r=0.519, P=0.002) and the GA/HbA1c ratio (r=0.409, P=0.016) were correlated with the fasting plasma glucose. Fasting C-peptide level arranged 4.22±3.22 ng/mL in T2DM, which was significantly above the values in T1DM (0.26±0.49 ng/mL). There were no significant correlation between HbA1c and fasting C-peptide level. However, GA and the GA/HbA1c ratio exhibited inverse correlations with fasting C-peptide level (r=-0.214, P=0.002; r=-0.516, P<0.001).ConclusionGA seems to more accurately reflects fasting plasma glucose level than HbA1c. GA, GA/HbA1c ratio appear to reflect insulin secretory function.

Serum Glycated Albumin and GA/HbA1c Ratio as a New Markers of Glycemic Control in Children with Type 1 Diabetes Mellitus

“…Takahashi et al and Selvin et al agreed with us about the strong relationship between BS readings both FBS and PPS, and both HbA1C & GA [10,18].…”

Analysis of free drug fractions in human serum by ultrafast affinity extraction and two-dimensional affinity chromatography

“…The pooled serum was prepared using de-identified and pre-existing samples from diabetic patients who had HbA1c levels ranging from 8.1%-12.6%, with this pooled serum having an HSA content of 44 g/L (provided by W. Clarke, Johns Hopkins University School of Medicine, Baltimore, MD, USA; this work was determined to be exempt from IRB review by the Johns Hopkins School of Medicine, according to the Code of Federal Regulations - 45 CFR 46.101 b). Based on their HbA1c levels, the clinical samples that were used to prepare this pooled serum had estimated glycated HSA levels in the range of 21–38% and glucose concentrations of 10–20 mM [33,34]. The commercial samples of normal human serum were estimated through a similar approach and from their glucose concentrations to have HbA1c levels of 4–5% and glycated HSA levels of 6–9%.…”

Sabina Galiniak, Grzegorz Bartosz, Izabela Sadowska-Bartosz

“…BSA has a 76% similarity in amino acid sequence to human serum albumin (HSA), which is the most abundant human plasma protein (Arasteh et al 2014). Glycated albumin may be a useful and specific marker of glycemia for pediatric diabetic patients (Lee et al 2013), diabetic hemodialysis patients (Inaba et al 2007), diabetic patients with cardiovascular complications (Sato et al 2013), diabetic patients with advanced chronic kidney disease (Vos et al 2011) and patients with coronary artery disease (Ma et al 2015).…”