Sex‐Dependent Neutralizing Humoral Response to<i>Schistosoma mansoni</i>28GST Antigen in Infected Human Populations

Remoué, Franck; Rogerie, F; Gallissot, Marie-Claire; Guyatt, Helen; Neyrinck, J.L.; Diakkhate, M.-M.; Niang, M.; Butterworth, Anthony E.; Auriault, C.; Càpron, André; Riveau, Gilles

doi:10.1086/315454

Cited by 36 publications

(29 citation statements)

References 15 publications

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“…1,2 However, the intensity of infection may also be related to behavioral routines (water contact), 3,4 age, 5 and sex. 6,7 Individual susceptibility that is associated with the immunologic 8,9 and genetic profiles [10][11][12] of each individual living in the endemic area is also important.…”

Section: Introductionmentioning

confidence: 99%

High levels of IgG4 to Schistosoma mansoni egg antigens in individuals with periportal fibrosis.

Silveira¹,

Bethony²,

Gazzinelli³

et al. 2002

The American Journal of Tropical Medicine and Hygiene

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Section: Introductionmentioning

confidence: 99%

High levels of IgG4 to Schistosoma mansoni egg antigens in individuals with periportal fibrosis.

Silveira¹,

Bethony²,

Gazzinelli³

et al. 2002

The American Journal of Tropical Medicine and Hygiene

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“…Interestingly, the type of antibody response elicited after immunization with phagedisplayed Sm28GST is comparable to that elicited after a DNA vaccination with Sm28GST-encoding DNA, which is known to confer significant protection (12). Similarly, a recent study in northern Senegal showed that a low level of S. mansoni infection in the male population correlated with a high level of neutralizing IgG3 antibody against Sm28GST in their sera (22). Although we did not check whether the IgG3 antibody elicited by phage-displayed Sm28GST in male mice is a neutralizing antibody, the response seems to be comparable to those in humans.…”

Section: Discussionmentioning

confidence: 70%

Expression of a 28-Kilodalton Glutathione S -Transferase Antigen of Schistosoma mansoni on the Surface of Filamentous Phages and Evaluation of Its Vaccine Potential

Rao

Kalyanasundaram

2003

Clin Vaccine Immunol

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A cloning and expression system that allows display of proteins on the surface of filamentous phages was exploited to display a 28-kDa glutathione S-transferase (Sm28GST) antigen of the human parasite Schistosoma mansoni. The phage-displayed Sm28GST (pdGST) was immunoreactive and was recognized by immune sera, suggesting that the Sm28GST protein displayed on the surface of phages potentially maintains native conformation. Subsequent immunization studies showed that mice can develop high titers of antibodies against pdGST and do not require any additional adjuvant for immunization. Isotype analysis suggested that the pdGST immunization predominantly induced immunoglobulin G2b (IgG2b), IgG3, and IgM anti-GST antibodies in mice. Furthermore, the pdGST immunization was found to confer about 30% protection after a challenge infection with 100 cercariae of S. mansoni in BALB/c mice. These findings suggest that phage display is a simple, efficient, and promising tool to express candidate vaccine antigens for immunization against infectious agents.

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“…In addition, we have previously demonstrated during human schistosomiasis that immune response to 28GST was gender dependent, with a specificity of epitope recognition (22,23). The binding of testosterone to 28GST could influence the orientation of the specific immune response according to the FIG.…”

Section: Vol 70 2002mentioning

confidence: 97%

Functional Specific Binding of Testosterone toSchistosoma haematobium28-Kilodalton GlutathioneS-Transferase

et al. 2002

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During parasitic disease such as schistosomiasis, sex hormones have an important influence on the age-and gender-dependent level of infection. Since mammal glutathione S-transferase (GST) has the ability to bind hormones and particularly sexual steroids to influence their transport, metabolism, and physiological action, we have evaluated the capacity of testosterone to bind the 28-kDa GST of the Schistosoma haematobium parasite (Sh28GST). For the first time, we have demonstrated a specific binding of testosterone to parasite GST protein with high affinity (K d ‫؍‬ 2.57 ؋ 10 ؊7 M). In addition, we have assessed the effect of this binding on Sh28GST enzymatic activity, a mechanism closely associated with the reduction of Schistosoma fecundity. We showed that testosterone has the functional ability to inhibit the Sh28GST enzymatic activity in a dose-dependent manner, suggesting that this hormone could be directly involved in an antifecundity mechanism. This effect seemed to be related to the binding of testosterone to one peptide involved in the enzymatic site (i.e., amino acids 24 to 43). During human infection, binding of sexual hormones to Schistosoma Sh28GST could play a key role in parasite metabolism, especially the decrease of fecundity, and could be involved in the sex-dependent immune response to Sh28GST that we have previously observed in infected adults.Sex hormones seem to have an influence on the level of parasitic infection. Indeed, gender-dependent patterns of prevalence and intensity of infection after puberty have been observed for several parasite species (5). It has been suggested that this effect seems to be associated with the regulatory roles of sex steroids on antiparasite immunity (2, 24). Generally, female hormones have an influence in increasing antibody response against specific antigen, which could explain the higher resistance of women against several parasitic infections (24, 25).During human Schistosoma infection, a chronic infection affecting 200 million individuals around the world, sex hormones and particularly the high level of testosterone after puberty could be an important immune modulator leading to the decrease of susceptibility to infection with age (10, 26). In mice infected by Schistosoma mansoni, fewer adult worms develop in males than in similarly infected females (9). In addition, female mice treated with testosterone before infection presented a reduction in worm burden, whereas no difference in antischistosome immune response was detected between treated and untreated animals (20). These authors suggest thus that effects of testosterone on specific immunity are not adequate to explain the differences in parasite loads observed between the sexes.Schistosomes could express a hormone receptor with homology to the testosterone receptor, which could explain the direct effect of testosterone on worm development (8). Interestingly, it has been demonstrated that testosterone treatment can affect not only the development of Nippostrongylus brasiliensis helminthic worms but als...

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Sex‐Dependent Neutralizing Humoral Response toSchistosoma mansoni28GST Antigen in Infected Human Populations

Cited by 36 publications

References 15 publications

High levels of IgG4 to Schistosoma mansoni egg antigens in individuals with periportal fibrosis.

High levels of IgG4 to Schistosoma mansoni egg antigens in individuals with periportal fibrosis.

Expression of a 28-Kilodalton Glutathione S -Transferase Antigen of Schistosoma mansoni on the Surface of Filamentous Phages and Evaluation of Its Vaccine Potential

Functional Specific Binding of Testosterone toSchistosoma haematobium28-Kilodalton GlutathioneS-Transferase

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