Sex Difference in the Daily Rhythm of Hepatic P450 Monooxygenase Activities in Rats Is Regulated by Growth Hormone Release

Furukawa, Tadashi; Manabe, Sunao; Watanabe, Toshiyuki; Sharyo, Satoru; Mori, Yuji

doi:10.1006/taap.1999.8808

Cited by 18 publications

(17 citation statements)

References 27 publications

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“…Since 1932, gender-related differences in the way by which animals metabolise drugs have been reported [15]; in this respect, a clear sexual dimorphism in the expression of hepatic genes, including some members of the CYP3A subfamily, has been documented in rodents and, particularly, in rats [1,13,20,24,28,33,42,45]. In cattle and other domestic animals, possible gendereffects on pharmacokinetics, including P450 drug metabolism, have already been described [4,6,8,9,17,18,36,40,52].…”

Section: Discussionmentioning

confidence: 99%

“…In our experimental conditions, the gender-effect was only observed at the catalytic activity level since immunoblotting showed no variation in the amount of CYP3A apoprotein; furthermore, RNA isolation and northern blot analysis of liver samples hybridised with bovine CYP3A specific oligonucleotide probes gave misleading results. In the rat, the sexual dimorphism in xenobiotic metabolism is regulated by growth hormone (GH), and such an effect was demonstrated at both catalytic activity and gene expression levels [13,33,45]. Besides, recombinant bovine somatothropin has been proven to modulate the activity of liver P450 enzymes in the rat [60].…”

Section: Discussionmentioning

confidence: 99%

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Effect of breed and gender on bovine liver cytochrome P450 3A (CYP3A) expression and inter-species comparison with other domestic ruminants

et al. 2005

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-The cytochrome P450 (P450) superfamily represents a group of relevant enzymes in the field of drug metabolism and several exogenous or constitutional factors contribute to regulate its expression. Cattle represent an important source of animal-derived food-products and studies concerning the P450 expression are needed for the extrapolation of pharmacotoxicological data from one species to another and for the evaluation of the consumer's risk associated with the consumption of harmful residues found in foodstuffs. In the present study, possible breed-, genderand species-differences in P4503A (the P450 subfamily more expressed in the human liver) expression were studied in vitro in Piedmontese (PDM) and Limousin (LIM) meat cattle breeds of both sexes and in domestic Ruminants (cattle, sheep and goats). Cytochrome P450 and P4503A contents as well as CYP3A-dependent drug metabolising enzymes (DME) were measured in liver microsomes. Significant lower levels of P450 (P < 0.001) and P4503A (P < 0.05) contents were observed in PDM vs. LIM of both sexes; the P4503A-dependent DME activities were significantly (P values ranging from 0.05 up to 0.001) higher in PDM cattle, particularly in males. A gendereffect in DME activities was noticed (P < 0.05) only in PDM male cattle. With regards to the species, the expression of both P4503A apoprotein and some of the related DME activities were more pronounced in sheep (P < 0.01 vs. cattle) and in goats (P < 0.05 vs. sheep; P < 0.01 vs. cattle) than in cattle. The significant differences in P4503A expression observed in LIM and PDM cattle are consistent with previously published data on strain-and breed-differences pointed out in rats and men. As far as a possible sex-effect is concerned, no clear-cut evidence is likely to be drawn. Finally, P4503A expression was more relevant in small ruminants. ruminants / liver drug metabolism / CYP3A / gender / breed

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Effect of breed and gender on bovine liver cytochrome P450 3A (CYP3A) expression and inter-species comparison with other domestic ruminants

et al. 2005

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“…To verify the sex difference in the circadian rhythms of hepatic P450 activities in the present study, P450 activities and P450 content were measured using microsomal fractions which were prepared from the liver samples collected at ZT6 (nadir time of the rhythmic P450 activities) and ZT18 (peak time of the rhythmic P450 activities) in males and females (Furukawa et al, 1999c). The thawed liver samples were mixed with 1.15% potassium chloride of 3-fold volume of the sample and homogenized on ice.…”

Section: Measurements Of Hepatic Phase I Drug-metabolizing Enzyme Actmentioning

confidence: 99%

“…Moreover, we have previously reported that the circadian rhythms of hepatic P450 activities in rats are obvious in males but not in females (Furukawa et al, 1999c). In mice, the circadian clock components, Cryptochrome 1 and 2 (Cry1 and Cry2), are suggested as a prerequisite for sustaining sex dimorphism in the liver metabolism (Bur et al, 2009).…”

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confidence: 98%

Sex and circadian modulatory effects on rat liver as assessed by transcriptome analyses

et al. 2011

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“…Propofol is mainly metabolized by hepatic microsomal systems (Court et al, 2001). Circadian fluctuations have been reported in rat hepatic enzyme activity with high values in the dark (activity) phase and low values in the light (resting) phase (Furukawa et al, 1999). Time of daydependent differences in the duration of propofol anesthesia may theoretically be due to circadian rhythms in hepatic metabolism of this drug.…”

Section: Chronopharmacology Of Propofol Anesthesiamentioning

confidence: 99%

Reciprocal Relationships between General (Propofol) Anesthesia and Circadian Time in Rats

Challet

Gourmelen

Pévet

et al. 2006

Neuropsychopharmacol

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Several common postdischarge symptoms, such as sleep disorders, headache, drowsiness or general malaise, evoke disturbances of circadian rhythms due to jet lag (ie crossing time zones) or shift work rotation. Considering that general anesthesia is associated with numerous effects on the central nervous system, we hypothesized that it may also act on the circadian timing system. We first determined the effects of the circadian timing on general anesthesia. We observed that identical doses of propofol showed marked circadian fluctuations in duration of effects, with a peak at the middle of the resting period (ie 7 h after lights on). Then, we examined the effects of general anesthesia on circadian timing, by analysing stable free-running circadian rhythms (ie in constant environmental conditions), an experimental approach used widely in circadian biology. Free-running rats were housed in constant darkness and temperature to assess possible phase-shifting effects of propofol anesthesia according to the time of the day. When administered around (72 h) the daily rest/activity transition point, a 30-min propofol anesthesia induced a 1-h phase advance in the free-running rest-activity rhythm, while anesthesia had no significant resetting effect at other times of the day. Anesthesia-induced hypothermia was not correlated with the phase-shifting effects of propofol anesthesia. From our results, anesthesia itself can reset circadian timing, and acts as a synchronizing cue for the circadian clock. Neuropsychopharmacology (2007) 32, 728-735.

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Sex Difference in the Daily Rhythm of Hepatic P450 Monooxygenase Activities in Rats Is Regulated by Growth Hormone Release

Cited by 18 publications

References 27 publications

Effect of breed and gender on bovine liver cytochrome P450 3A (CYP3A) expression and inter-species comparison with other domestic ruminants

Effect of breed and gender on bovine liver cytochrome P450 3A (CYP3A) expression and inter-species comparison with other domestic ruminants

Sex and circadian modulatory effects on rat liver as assessed by transcriptome analyses

Reciprocal Relationships between General (Propofol) Anesthesia and Circadian Time in Rats

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