Sex-sorted bovine spermatozoa and DNA damage: II. Dynamic features

Gosálvez, J.; Ramírez, Mario A.; López‐Fernández, Carmen; Crespo, F.; Evans, K. M.; Kjelland, Michael E.; Moreno, J. F.

doi:10.1016/j.theriogenology.2010.09.011

Cited by 39 publications

(27 citation statements)

References 29 publications

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“…In fact, this is what we have observed in bovine where, as in deer, the baseline SDF is greater in FT-Conv than in FT-Sex-Sort samples, but the rate of SDF is greater in FT-Sex-Sort samples than in FT-Conv samples. This is an interesting effect because when both curves are plotted together, a crossover in the tendency of SDF (termed the crossover positioning time; Gosálvez et al, 2011b) for both conventional and sex-sorted samples is produced (Figure 4a). This aforementioned crossover positioning time was not produced in the deer sperm Table 2.…”

Section: Discussionmentioning

confidence: 99%

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DNA fragmentation kinetics and postthaw motility of flow cytometric-sorted white-tailed deer sperm1

Kjelland

González-Marín

Gosálvez

et al. 2011

Journal of Animal Science

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This study examined DNA damage and postthaw motility of white-tailed deer sperm (n = 28) before and after sex selection and conventional sorting using MoFlo XDP SX flow cytometry. Semen samples from the same individuals were treated in 4 different ways: 1) chilled-extended sperm samples (without glycerol); 2) cryopreserved conventional samples, samples directly cryopreserved after the addition of extenders; 3) cryopreserved conventionally sorted samples, sorted samples to remove the dead sperm subpopulation; and 4) cryopreserved sex-sorted samples; sorted samples to remove the dead sperm subpopulation and separation of X- and Y-chromosome-bearing sperm. In all the cases (n = 6), conventional samples showed decreased postthaw motilities (43 ± 26%) when compared with X-sorted samples (59 ± 20%; P < 0.05) and Y-sorted samples (54 ± 20%; P > 0.05). The DNA fragmentation baseline was <5% for frozen-thawed conventional samples, but even less after sex sorting and conventional sorting: 2.4 and 1.7%, respectively. On the other hand, conventional samples showed greater (P < 0.05) DNA fragmentation than the sex-sorted sperm (n = 6) at 96 h (average of 4.8 ± 4.5% and 5.3 ± 4%, respectively). Conventionally sorted samples (n = 8) did not have greater (P > 0.05) DNA fragmentation when compared with the sex-sorted samples. Fragmentation of DNA on X-chromosome and Y-chromosome-bearing sorted sperm were not significantly different (n = 10, P > 0.05) after 96 h (2.6 ± 3.6% and 2.2 ± 0.5%, respectively). Future research should be implemented for examining the fertilizing potential of sex-sorted white-tailed deer sperm (e.g., AI fertility trials).

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Section: Discussionmentioning

confidence: 99%

“…Comparison of the dynamic behavior of conventional (unfilled circles) and sex-sorted sperm samples (filled circles) in a) bulls, modified fromGosálvez et al (2011b), and b) white-tailed deer. CPT = crossover positioning time.…”

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confidence: 99%

DNA fragmentation kinetics and postthaw motility of flow cytometric-sorted white-tailed deer sperm1

Kjelland

González-Marín

Gosálvez

et al. 2011

Journal of Animal Science

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“…The high-speed flow cytometry sorting process could reduce sperm lifespan (e.g. in terms of DNA fragmentation rates, as observed by Gosálvez et al 2011) and increase ROS content during long fertilisation times (Johnson et al 1999;Garner 2006). The initial hypothesis was that M199 medium could have an advantage over the other two media in terms of minimising osmotic stress to MII oocytes as result of passage from IVM to IVF, protecting oocytes and sperm against oxidative stress through antioxidants and preventing oocyte aging.…”

Section: Discussionmentioning

confidence: 99%

“…flow cytometry) that SS have undergone. The high speed during the sorting process could reduce sperm lifespan (Gosálvez et al 2011) and increase ROS content during long fertilisation periods (Garner et al 2013;Rath et al 2013). A decrease in sperm chromatin stability could easily be associated with mechanical injury, enhanced via DNA exposure to Hoechst 33342 and/or laser illumination (Garner 2006).…”

Section: Introductionmentioning

confidence: 99%

Comparison of different fertilisation media for an in vitro maturation–fertilisation–culture system using flow-cytometrically sorted X chromosome-bearing spermatozoa for bovine embryo production

Ferré

Bogliotti

Chitwood

et al. 2016

Reprod. Fertil. Dev.

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High demand exists among commercial cattle producers for in vitro-derived bovine embryos fertilised with female sex-sorted spermatozoa from high-value breeding stock. The aim of this study was to evaluate three fertilisation media, namely M199, synthetic oviductal fluid (SOF) and Tyrode's albumin-lactate-pyruvate (TALP), on IVF performance using female sex-sorted spermatozoa. In all, 1143, 1220 and 1041 cumulus-oocyte complexes were fertilised in M199, SOF and TALP, respectively. There were significant differences among fertilisation media (P < 0.05) in cleavage rate (M199 = 57%, SOF = 71% and TALP = 72%), blastocyst formation (M199 = 9%, SOF = 20% and TALP = 19%), proportion of Grade 1 blastocysts (M199 = 15%, SOF = 52% and TALP = 51%), proportion of Grade 3 blastocysts (M199 = 58%, SOF = 21% and TALP = 20%) and hatching rates (M199 = 29%, SOF = 60% and TALP = 65%). The inner cell mass (ICM) and trophectoderm (TE) cells of Day 7 blastocysts were also affected by the fertilisation medium. Embryos derived from SOF and TALP fertilisation media had higher numbers of ICM, TE and total cells than those fertilised in M199. In conclusion, fertilisation media affected cleavage rate, as well as subsequent embryo development, quality and hatching ability. SOF and TALP fertilisation media produced significantly more embryos of higher quality than M199.

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“…Reduction in the dose rate from 10 × 10 6 to 2.1 × 10 6 sperm caused an 8.3% decrease in conception rate in frozen CON semen, but a 13.6% decrease for frozen SS semen (DeJarnette et al, 2011). In addition, it has been shown that the increases over time in sperm DNA fragmentation in frozen-thawed semen samples was faster for SS semen than CON semen (Gosálvez et al, 2011b). Implementation of liquid SS semen is not without its own challenges.…”

Section: Discussionmentioning

confidence: 99%

Application of liquid semen technology improves conception rate of sex-sorted semen in lactating dairy cows

2014

Journal of Dairy Science

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The objective was to compare reproductive performance of liquid sex-sorted (SS) semen with that of conventional (CON) semen in lactating dairy cows. Between 2011 and 2013, commercial dairy herds (n = 101, 203, and 253 for 2011, 2012, and 2013, respectively) with predominantly Holstein-Friesian cows were enrolled in a contract mating program to produce surplus heifers for export using liquid SS semen. During the spring mating period, each herd was allocated with liquid SS semen at 50% of its daily requirement and the remaining daily requirement was allocated with CON liquid semen. Sperm for producing SS semen was sorted by Sexing Technologies NZ Ltd. (Hamilton, New Zealand) and then packaged using the liquid semen technology of LIC (Hamilton, New Zealand) at a dose of 1 × 10(6) sperm. Artificial insemination (AI) with liquid SS semen was carried out between 43 and 46 h after collection. Conventional semen straws contained 1.25 × 10(6), 1.75 × 10(6), or 2 × 10(6) sperm for semen to be used on d 1, 2, or 3 after collection, respectively. Only CON inseminations on the same days as when SS semen was used were included in the comparison. Herd managers biased usage of SS semen toward cows with a longer postpartum interval before the mating start date (64.0 vs. 62.8 d), cows of higher genetic merit (NZ$107.0 vs. NZ$98.4), younger cows (5.1 vs. 5.2 yr), and cows in which they had more confidence of being genuinely in estrus as measured by a lower percentage of short returns between 1 and 17 d (5.3 vs. 7.5%). After adjusting for these factors, the estimated difference in nonreturn rate between AI with SS and CON semen over the 3 seasons was -3.8 percentage points (SS = 70.2% vs. CON = 74.0%; SS/CON = 94.9%). The estimated maximum difference in calving rate per AI between SS and CON semen was -3.1 percentage points for 2011 (SS = 51.2% vs. CON = 54.3%; SS/CON = 94.3%) and -3.0 percentage points for 2012 (SS = 49.7% vs. CON = 52.6%; SS/CON = 94.5%). Calving data for 2013 were not yet available. The percentage of heifer calves born to AI with SS semen was 87.0% for 2011 and 85.8% for 2012, both of which were lower than the expectation of 90% mainly due to misidentification of calf dams in seasonal dairy herds calving on pasture. In summary, results in this report showed that liquid SS semen only required half the dose rate of frozen SS semen to achieve a reproductive performance of over 94% of CON semen in lactating dairy cows. Careful planning and a robust distribution network are required to avoid semen wastage and to maximize the benefit of liquid SS semen.

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Sex-sorted bovine spermatozoa and DNA damage: II. Dynamic features

Cited by 39 publications

References 29 publications

DNA fragmentation kinetics and postthaw motility of flow cytometric-sorted white-tailed deer sperm1

DNA fragmentation kinetics and postthaw motility of flow cytometric-sorted white-tailed deer sperm1

Comparison of different fertilisation media for an in vitro maturation–fertilisation–culture system using flow-cytometrically sorted X chromosome-bearing spermatozoa for bovine embryo production

Application of liquid semen technology improves conception rate of sex-sorted semen in lactating dairy cows

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