Geothermal resource quantification requires underground temperature and volume information, which can be challenging to accurately assess at the regional scale. The analytical solution for steady-state heat conduction with internal heat generation is often used to calculate temperature at depth, while geological models can provide volume information. Both approaches were originally combined in a single 3D geological model, in which the underground temperature is directly computed, to accurately evaluate geothermal resources suitable for power generation in the St.Lawrence Lowlands sedimentary basin covering 18,000 km 2 in Quebec, Canada, and improve methods for geothermal resource quantification. This approach, used for the first time at such a large scale, allowed to determine the volume of each thermal unit providing a detail assessment of resource depth, temperature and host geological formation. Only 5% of geothermal resources at a temperature above 120 °C that is suitable for power generation were shown to be hosted in the Cambro-Ordovician sedimentary rock sequences at a depth of 4 to 6 km, while 95% of the resource is hosted by the underlying Precambrian basement.