Sheathless Capillary Electrophoresis/Electrospray Mass Spectrometry Using a Carbon-Coated Fused-Silica Capillary

Chang, Yan Zin; Her, Guor‐Rong

doi:10.1021/ac990535e

Cited by 76 publications

(64 citation statements)

References 22 publications

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“…Sheath liquid provides (i) electrical contact with the outlet end of the separation capillary, (ii) appropriate flow rate adapted to source configuration, and (iii) suitable solvent conditions for ionization and evaporation, independent on the nature of the BGE. Several sheathless interfaces [11][12][13][14][15][16][17] have also been investigated but still suffer from some important drawbacks (increased band broadening, higher background noise due to the lower total flow rate, lack of robustness).…”

Section: Introductionmentioning

confidence: 99%

Coupling CE with atmospheric pressure photoionization MS for pharmaceutical basic compounds: Optimization of operating parameters

Schappler¹,

Guillarme²,

Prat³

et al. 2007

Electrophoresis

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The use of CE coupled with MS (CE-MS) has evolved as a useful tool to analyze charged species in small sample volumes. Because of its sensitivity, versatility and ease of implementation, the ESI interface is currently the method of choice to hyphenate CE to MS. An alternative can be the atmospheric pressure photoionization (APPI) source, however, numerous parameters must be optimized for its coupling to CE. After evaluation of the sheath liquid composition and the CE capillary outlet position, an experimental design methodology was assessed for optimizing other ionization source parameters, such as sheath liquid flow rate, drying gas flow rate and temperature, nebulizing gas pressure, vaporizer temperature, and capillary voltage. For this purpose, a fractional factorial design (FFD) was selected as a screening procedure to identify factors which significantly influence sensitivity and efficiency. A face-centered central composite design (CCD) was then used to predict and optimize sensitivity, taking into account the most relevant variables. Sensitivity was finally evaluated with the optimized conditions and height-to-noise ratios (H/N) around 10 were achieved for an injection of 200 ng/mL of each analyte.

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Section: Introductionmentioning

confidence: 99%

Coupling CE with atmospheric pressure photoionization MS for pharmaceutical basic compounds: Optimization of operating parameters

Schappler¹,

Guillarme²,

Prat³

et al. 2007

Electrophoresis

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show abstract

“…A means to close the CE electrical circuit for analyte separation while simultaneously providing an electrical potential to the spray tip is necessary. The common interfaces can be divided into three categories: sheath-flow 16,[18][19][20]24,27,32,37,38,40,43,47,50,[53][54][55][56]60,66 and sheathless 15,17,25,26,[28][29][30]34,36,44,45,48,49,61 and liquid-junction interfaces. [92][93][94] In the sheath-flow design, a coaxial sheath liquid, usually a hydroorganic mixture, mixes with the capillary eluent at the outlet end of the capillary.…”

Section: ·1 Esi-msmentioning

confidence: 99%

Recent Developments in Capillary Electrophoresis–Mass Spectrometry of Proteins and Peptides

Monton

Terabe

2005

ANAL. SCI.

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Many researchers have invested considerable efforts toward improving capillary electrophoresis (CE)-mass spectrometry (MS) systems so they can be applied better to standard analyses. This review highlights the developments in CE-MS of proteins and peptides over the last five years. It includes the developments in interfaces, sample-enrichment techniques, microfabricated devices, and some applications, largely in capillary zone electrophoresis (CZE), capillary isoelectric focusing (CIEF) and capillary isotachophoresis formats.

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“…Foremost is the robust transfer of analytes from the separations (CE) capillary to the inlet of the mass spectrometer via electrospray. Many different types of ESI interfaces have been reported for the coupling of CE to MS [18,[22][23][24][25][26][27][28][29][30][31][32][33][34][35]. In general they fall into three classes: (i) sheathless interfaces, wherein the separations capillary is connected directly to an electrospray emitter (tip), (ii) liquid-junction interfaces, where there is a small liquidfilled gap between the separations capillary and the emitter, and (iii) sheath-flow interfaces, wherein the end of the separations capillary is enclosed within another capillary through which flows a constant supply of sheath liquid.…”

Section: Introductionmentioning

confidence: 99%

An automated, sheathless capillary electrophoresis‐mass spectrometry platform for discovery of biomarkers in human serum

Sassi¹,

Andel²,

Bitter³

et al. 2005

Electrophoresis

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An automated, sheathless capillary electrophoresis-mass spectrometry platform for discovery of biomarkers in human serumA capillary electrophoresis-mass spectrometry (CE-MS) method has been developed to perform routine, automated analysis of low-molecular-weight peptides in human serum. The method incorporates transient isotachophoresis for in-line preconcentration and a sheathless electrospray interface. To evaluate the performance of the method and demonstrate the utility of the approach, an experiment was designed in which peptides were added to sera from individuals at each of two different concentrations, artificially creating two groups of samples. The CE-MS data from the serum samples were divided into separate training and test sets. A pattern-recognition/feature-selection algorithm based on support vector machines was used to select the mass-to-charge (m/z) values from the training set data that distinguished the two groups of samples from each other. The added peptides were identified correctly as the distinguishing features, and pattern recognition based on these peptides was used to assign each sample in the independent test set to its respective group. A twofold difference in peptide concentration could be detected with statistical significance (pvalue , 0.0001). The accuracy of the assignment was 95%, demonstrating the utility of this technique for the discovery of patterns of biomarkers in serum.

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Sheathless Capillary Electrophoresis/Electrospray Mass Spectrometry Using a Carbon-Coated Fused-Silica Capillary

Cited by 76 publications

References 22 publications

Coupling CE with atmospheric pressure photoionization MS for pharmaceutical basic compounds: Optimization of operating parameters

Coupling CE with atmospheric pressure photoionization MS for pharmaceutical basic compounds: Optimization of operating parameters

Recent Developments in Capillary Electrophoresis–Mass Spectrometry of Proteins and Peptides

An automated, sheathless capillary electrophoresis‐mass spectrometry platform for discovery of biomarkers in human serum

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