Shoot regeneration, re-flowering and post flowering survival in bamboo inflorescence culture

Abstract

In vitro grown inflorescences of Bambusa edulis were used to investigate the process of vegetative shoot growth in detail. The findings revealed that auxins and ACC could be significant growth regulators in this process. Overall, auxins [NAA, indolebutyric acid (IBA), and 2,4-dichlorophenoxyacetic acid (2,4-D D )] induced inflorescences to grow vegetative shoots. However, the efficiency of shoot regeneration varied. A greater percentage (27.3-34.5%) of inflorescences in the 5 mg l )l NAA, 10 mg l )l NAA, and 1 mg l )l 2,4-D D treatments formed more vegetative shoots than those exposed to other treatments. IBA promoted shoot regeneration less effectively than NAA and 2,4-D D . Fifty percent of regenerated vegetative shoots flowered after 2 months when the medium was supplemented with 5 mg l )l NAA. All shoots that received 1 mg l )l 1-amino-cyclopropane-1-carboxylic acid (ACC) flowered in 5 mg l )l NAA medium. Rooted plantlets were used to examine their survival following in vitro flowering. All plantlets with vegetative shoots, even those with inflorescences, survived and grew.Abbreviations: ACC -1-amino-cyclopropane-1-carboxylic acid; 2,4-D D -2,4-dichlorophenoxyacetic acid; IBA -indolebutyric acid; MS -Murashige and Skoog basal medium; NAA -naphthaleneacetic acid; TDZthidiazuron

In vitro induction of inflorescence in Dioscorea zingiberensis

“…BA has been reported to be effective in floral induction of a number of plant species, such as orchids (Chang and Chang. 2003;Naor et al 2004), bitter melon (Wang et al 2001), and bamboo (Lin et al 2005b). In this study, the influence of BA on in vitro flowering of D. zingiberensis was remarkable.…”

“…Floral induction was obtained via somatic embryogenesis and organogenesis in bamboo (Lin et al 2003(Lin et al , 2004(Lin et al , 2005b and Panax ginseng (Tang 2000). The combination of BA and GA 3 induced direct in vitro flowering in ginseng (Lin et al 2005a).…”

In Vitro Propagation of an Edible Bamboo Bam-busa Bambos and Assessment of Clonal Fidelity through Molecular Markers

“…1d & e). The role of IBA and NAA in root induction had been reported earlier in other bamboos like Bambusa vulgaris by [11]; in B. oldhamii by [12] and Dendrocalamus asper by [13]. Complete plantlets were hardened on soil: sand: farmyard manure (1:1:1) by gradually lowering the relative humidity and were successfully transferred from in vitro to natural environmental conditions with 80% survival rate.…”

Flowering of Woody Bamboo in Tissue Culture Systems

“…When using in vitro inflorescences as explants, auxin-only medium increased the floret size, and also induced adventitious roots and caused 35% more vegetative shoots to emerge. These rooted vegetative plantlets could be transplanted to the greenhouse and survive (Lin et al, 2005). These results indicated that auxin plays a negative role in bamboo flowering and inflorescence proliferation in vitro .…”

“…While only self-crosses have thus far successfully produced seeds in vitro (Nadgauda et al, 1990), advances in technology, new induction protocols, or alternative hybridization strategies can further the realization of this goal. For example, D. latiflorus and B. edulis plantlets induced to flower in vitro were successfully transferred to the greenhouse, where they continued flowering (Zhang and Wang, 2001; Lin C. C. et al, 2003; Lin et al, 2005). Perhaps parental bamboo accessions could be induced to flower in vitro and transplanted to the greenhouse for further hybridization with other bamboos that are flowering, whether they were induced in vitro or in vivo .…”