Short Communication Association of APOA1 and APOA5 polymorphisms and haplotypes with lipid parameters in a Brazilian elderly cohort

Abstract

ABSTRACT.Apolipoproteins have an important role in lipid metabolism and transport. Polymorphisms in the APOA1/C3/A4/ A5 gene cluster have been associated with lipid alterations and cardiovascular diseases. We investigated APOA1 XmnI, APOA5 S19W, and APOA5 -1131T>C polymorphisms in 377 individuals from a cohort of a longitudinal Brazilian elderly study. Allele frequencies, genotype distribution, and association with major morbidities as well as with lipids, creatinine, albumin, urea, glycated hemoglobin, and fasting glucose serum levels were investigated. Linkage disequilibrium and haplotype associations were also analyzed. This is the first time that haplotypes involving these polymorphisms were evaluated. Genotyping was performed by PCR-RFLP. Minor allele frequencies were 0.119, 0.071, and 0.158 for XmnI, S19W, and -1131T>C polymorphisms, respectively. We found a significant association of the -1131C allele with low LDL-C levels. We also observed that XmnI and S19W polymorphisms were in linkage disequilibrium. The C/G haplotype, which is composed of the wild-type allele of XmnI and the minor allele of S19W, was associated with high total cholesterol serum levels in this elderly population. We conclude that the -1131T>C polymorphism and the C/G haplotype, including XmnI and S19W polymorphisms, are associated with alterations in lipid levels and may be risk factors for cardiovascular disease in the Brazilian elderly.

Association of two common polymorphisms of apolipoprotein A5 gene with metabolic syndrome indicators in a North Iranian population, a cross-sectional study

“…The frequency of the G Allele of ApoA5 c.56C>G polymorphism in our population (5%) is close to the corresponding values reported for Caucasians such as Danish 6% [24], Turks 6% [47] and British 6% [48], as well as populations like Brazilians 7% [32], but it is less than that obtained for Costaricans 10.2% [49]. The reason for similarities between results we obtained for our Iranian population and those obtained for Caucasians can be attributed to the close ethnic relationship between the two populations.…”

“…These values are close to allele C frequency reported for other Caucasian populations, such as the Brazilians 16% [32] Hispanics 13-16% and Turks 13% [23] but differ from other ethnic population like East Asians 27-37% [29,33]. …”

Protein Profiles for Muscle Development and Intramuscular Fat Accumulation at Different Post-Hatching Ages in Chickens

“…Apolipoprotein A1 (apoA1) is the major apolipoprotein constituent of the high-density lipoprotein (HDL) and is involved in reverse cholesterol transport[ 69 ]. Variants in the apolipoprotein A1 ( APOA1 ) gene play an important role in the regulation of lipid transport[ 70 – 73 ]. Synthesis of APOAI in the skeletal muscle of hatchling chicks acts as a local lipid transporter for early post-hatching development[ 24 ].…”

Decreased expression of the APOA1–APOC3–APOA4 gene cluster is associated with risk of Alzheimer’s disease

“…Compared with other serum biomarkers of AD patients, 9 , 10 serum apolipoproteins APOA1, APOC3, and APOA4 seem to be more functional proteins: 1) three proteins can be used to predict the severity of AD more accurately than a single protein, 2) all the proteins affect the progression of AD, 66 , 67 and 3) the polymorphisms of these apolipoprotein genes may result in the pathogenesis and development of AD. 20 , 68 With more certain results, we hope that apolipoprotein-gene cluster of APOA1 , APOC3 , and APOA4 can be widely applied in the diagnosis of AD.…”