Short-term cold storage of encapsulated somatic embryos and retrieval of plantlets in grey orchid (Vanda tessellata (Roxb.) Hook. ex G.Don)

Manokari, M.; Rangan, Latha; Priyadharshini, S.; Jogam, Phanikanth; Shekhawat, Mahipal S.

doi:10.1007/s11240-020-01899-y

Cited by 25 publications

(12 citation statements)

References 63 publications

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“…The cultures of V. tessellata have been established following our previous work (Manokari et al, 2020). The hardening and acclimatization steps determine the successful development of micropropagation protocol for V. tessellata and considered as a maturation phase.…”

Section: Resultsmentioning

confidence: 99%

“…The mature and healthy pods (2–4 cm long) of Vanda tessellata were collected from the Auroville Botanical Garden, Puducherry, India. The cultures were established and plantlets were produced by following the recent report of Manokari, Latha, Priyadharshini, Phanikanth, and Shekhawat (2020) on in vitro propagation of V. tessellata . Briefly, the protocorms were developed from seeds on full strength Murashige and Skoog's (MS) medium (Murashige & Skoog, 1962), containing 2.0 mg L −1 6‐benzylaminopurine (BAP), and somatic embryogenic potential of protocorms was stimulated using MS medium containing 1.0 mg L −1 BAP and 0.5 mg L −1 indole‐3 acetic acid (IAA).…”

Section: Methodsmentioning

confidence: 99%

“…Statistical analysis of the data was done by ANOVA using statistical software "SPSS" version 17 (SPSS, Chicago). The results expressed are mean ± SE of three independent experiments.3 | RESULTS AND DISCUSSIONThe cultures of V. tessellata have been established following our previous work(Manokari et al, 2020). The hardening and acclimatization steps determine the successful development of micropropagation protocol for V. tessellata and considered as a maturation phase.…”

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confidence: 99%

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Micro‐morpho‐anatomical mechanisms involve in epiphytic adaptation of micropropagated plants of Vanda tessellata (Roxb.) Hook. ex G. Don

Manokari

Rasangam

Selvam

et al. 2020

Microscopy Res & Technique

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Vanda tessellata (Roxb.) Hook. ex G. Don. (grey orchid, family Orchidaceae) is an epiphytic orchid of horticultural importance and currently under threat due to overharvesting and habitat destruction. Micropropagation protocols were developed for the production of grey orchid but the survival success of in vitro regenerated plantlets is uncertain due to lack of understanding about the adaptation mechanism during hardening. The present study describes the structural adaptation mechanism of V. tessellata when the in vitro regenerated plantlets were acclimatized under the greenhouse conditions. Light microscopy has been implicated to identify the adaptational alterations during in vitro to ex vitro transition of micropropagated plantlets.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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Micro‐morpho‐anatomical mechanisms involve in epiphytic adaptation of micropropagated plants of Vanda tessellata (Roxb.) Hook. ex G. Don

Manokari

Rasangam

Selvam

et al. 2020

Microscopy Res & Technique

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“…Hence, there is a need to consider the type of explants used. Potential explants include the use of seeds (Utami & Hariyanto, 2019) as previously studied by Adhikari & Pant (2019), Dulić et al (2019), Godo et al (2020), and Manokari et al (2021). However, this study differs from previous studies, we provided C. rochussenii orchid seeds taken directly from the wildlife reserve area of Bukit Rimbang and Baling wildlife reserve areas.…”

Section: Introductionmentioning

confidence: 99%

Ex-situ conservation of the native orchid Coelogyne rochusseni de Vriese from the Bukit Rimbang and Bukit Baling Wildlife Reserve Areas

Heriansyah¹,

Marlina²

2021

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The native orchid Coelogyne rochussenii de Vriese is under threat of extinction, so conservation measures need to be taken. In-situ conservation is constrained by time, resources and costs, and the appropriate conservation must be ex-situ. Therefore, This study aims to determine the most effective method for ex-situ conservation, especially basal medium and activated charcoal, in order to determine the fastest germination. The method used in this research was a factorial completely randomized design, consisting of four different basal medium treatments, Knudson C, Vacin and Went, Murashige and Skoog, and Hyponex + Vitamin medium. This treatment was combined with the application of activated charcoal at 4 different levels, with concentrations of 0.1,2.3 g/L-1, the parameters observed were germination day (day), germination percentage (%), and percentage of contamination (%). The results of this study concluded that the conservation of the Native orchid Coelogyne Rochussenii de Vriese using various growing mediums and activated charcoal had a significant effect on the growth of the embryo culture native orchid. Hyponex + Vitamin medium with 3 g/L-1 is able to accelerate germination days to 29.33 days, increase germination percentage to 92.06%, and reduce contamination to 0%.

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“…Increasing the length of rewarming time had no significant effect on survival, suggesting that the most critical events occur within the first minute of rewarming. Manokari et al (2021) established an efficient protocol for asymbiotic seed germination of the grey orchid (Vanda tessellata (Roxb.) Hook.…”

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confidence: 99%

Cryopreservation and In Vitro banking: a cool subject – Preface from the editors

Ochatt

Lambardi

Panis

et al. 2021

Plant Cell Tiss Organ Cult

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Short-term cold storage of encapsulated somatic embryos and retrieval of plantlets in grey orchid (Vanda tessellata (Roxb.) Hook. ex G.Don)

Cited by 25 publications

References 63 publications

Micro‐morpho‐anatomical mechanisms involve in epiphytic adaptation of micropropagated plants of Vanda tessellata (Roxb.) Hook. ex G. Don

Micro‐morpho‐anatomical mechanisms involve in epiphytic adaptation of micropropagated plants of Vanda tessellata (Roxb.) Hook. ex G. Don

Ex-situ conservation of the native orchid Coelogyne rochusseni de Vriese from the Bukit Rimbang and Bukit Baling Wildlife Reserve Areas

Cryopreservation and In Vitro banking: a cool subject – Preface from the editors

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