Short‐term exposure to 50 Hz ELF‐EMF alters the cisplatin‐induced oxidative response in AT478 murine squamous cell carcinoma cells

Bułdak, Rafał Jakub; Polaniak, Renata; Bułdak, Łukasz; Żwirska-Korczala, K; Skonieczna, Magdalena; Monsiol, Aleksandra; Kukla, Michał; Duława-Bułdak, Anna; Birkner, Ewa

doi:10.1002/bem.21732

Cited by 65 publications

(33 citation statements)

References 39 publications

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“…These results showed that cisplatin decreases the antioxidant power, so that it increases the peroxidation damages of the body, whose results were fairly in accorded with some studies' (Sahu BD et al, 2011;Buldak RJ et al, 2012;Gupta RK et al, 2013;Zhao YM et al, 2014). In this experiment, the oxidative stress indices in the pu-erh tea related groups changed to some extent compared to the cisplatin group, including the activities of SOD and GSH-PX and the levels of GSH were increased, while the levels of MDA were decreased.…”

Section: Discussionsupporting

confidence: 87%

Pu-erh Tea Powder Preventive Effects on Cisplatin-Induced Liver Oxidative Damage in Wistar Rats

Zheng¹,

Wang²,

Li³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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Asian Pac J Cancer Prev, 15 (17), 7389-7394 IntroductionChemotherapy is one of the major means for malignancy. It seeks to shrink tumors, decrease cancerrelated symptoms, advance quality of life, lengthen life spans of patients, and so on. Many studies have shown the effectiveness and feasibility of chemotherapy for cancers species involving the lung, pancreas, liver, large intestine, and so on. Conversely, treatment with anticancer drugs can also do harm to patients' normal tissues and organs, and those toxicities may lead to a worsened quality of life and shortened survival.Cisplatin (cis-diamminedichloroplatinumⅡ, CDDP) is one of the main chemotherapeutics, which is widely used for the treatment of various malignant solid tumors such as ovarian, lung, testicular and head and neck cancers (Pfister et al., 2010;Dimri et al., 2013; Dua et al., 2013;Besse et al., 2014 AbstractBackground: Chemotherapy is one of the major means for control of malignancies, with cisplatin (CDDP) as one of the main agents, widely used for the treatment of various malignant solid tumors. However, prevention of hepatotoxicity from cisplatin is one of the urgent issues in cancer chemotherapy. In this study, we aimed to investigate the effects of pu-erh tea on hepatotoxicity through body weight and tissue antioxidant parameters like, liver coefficient, serum alanine aminotransferase (ALT), serum aspartate aminotransferase (AST), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), malondialdehyde(MDA) and glutathione (GSH) levels, and light microscopic evaluation by histological findings. Materials and Methods: The rats were randomly divided into five groups: Control (n=10), cisplatin (3 mg/kg p.i., n=10), cisplatin+pu-erh (0.32 g/kg/day i.g., n=10), cisplatin+pu-erh (0.8 g/kg/day i.g., n=10) and cisplatin+pu-erh (1.6 g/kg/day i.g., n=10). Pu-erh tea powder was administrated for 31 consecutive days. The rats were sacrificed at the end on the second day after a single dose of cisplatin treatment for measuring indices. Results: Pu-erh tea powder exhibited a protective effect by decreasing MDA and GSH and increasing the SOD and GSH-PX levels and GSH-PX/MDA ratio in camparison with the control group. Besides, pu-erh tea was also able to alleviate the pathological damage to some extent. Conclusion: Pu-erh tea powder is protective against cisplatin-induced liver oxidative damages, especially at the medium dosage (0.8 g/kg/d).

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Section: Discussionsupporting

confidence: 87%

Pu-erh Tea Powder Preventive Effects on Cisplatin-Induced Liver Oxidative Damage in Wistar Rats

Zheng¹,

Wang²,

Li³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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“…Buldak et al. found not only an increased SOD activity but also an increased GPx activity and a reduced lipid peroxidation in AT478 exposed to ELF‐EMF of 50 Hz and 1 mT up to 72 H . Cellular adaptation to ELF‐EMF was also noted in pheochromocytoma‐derived cells exposed to 50 Hz and 0.1–1 mT, increased ROS production and decreased CAT activity were observed after 30 Min of exposure, but an opposite tendency for these parameters was observed after chronic exposure of 7 days .…”

Section: Resultsmentioning

confidence: 95%

“…Falone et al observed higher GSH levels, but higher GPx, glutathione S-transferase, and γ -glutamylcysteine synthetase activity and higher Glut expression in neuroblastoma cells after 50 Hz and 1 mT of exposure for 96 H [57]. Buldak et al found not only an increased SOD activity but also an increased GPx activity and a reduced lipid peroxidation in AT478 exposed to ELF-EMF of 50 Hz and 1 mT up to 72 H [58]. Cellular adaptation to ELF-EMF was also noted in pheochromocytoma-derived cells exposed to 50 Hz and 0.1-1 mT, increased ROS production and decreased CAT activity were observed after 30 Min of exposure, but an opposite tendency for these parameters was observed after chronic exposure of 7 days [47].…”

Section: Figmentioning

confidence: 99%

Effect of extremely low‐frequency electromagnetic fields on antioxidant activity in the human keratinocyte cell line NCTC 2544

Calcabrini

Mancini

Bellis

et al. 2016

Biotech and App Biochem

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Some epidemiological studies have suggested possible associations between exposure to extremely low-frequency electromagnetic fields (ELF-EMFs) and various diseases. Recently, ELF-EMF has been considered as a therapeutic agent. To support ELF-EMF use in regenerative medicine, in particular in the treatment of skin injuries, we investigated whether significant cell damage occurs after ELF-EMF exposure. Reactive oxygen species (ROS) production was evaluated in the human keratinocyte exposed for 1 H to 50 Hz ELF-EMF in a range of field strengths from 0.25 to 2 G. Significant ROS increases resulted at 0.5 and 1 G and under these flux densities ROS production, glutathione content, antioxidant defense activity, and lipid peroxidation markers were assessed for different lengths of time. Analyzed parameters of antioxidant defense and membrane integrity showed a different trend at two selected magnetic fluxes, with a greater sensitivity of the cells exposed to 0.5 G, especially after 1 H. All significant alterations observed in the first 4 H of exposure reverted to controls 24 H after suggesting that under these conditions, ELF-EMF induces a slight oxidative stress that does not overwhelm the metabolic capacity of the cells or have a cytotoxic effect.

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“…Data generated from several epidemiological studies on populations occupationally exposed to ELF-MF have been interpreted as indicative of increased risk and incidence of some diseases, including leukemia [Kheifets et al, 2009], neurodegenerative diseases [Park et al, 2005], and some brain tumors [Repacholi and Greenebaum, 1999]. In vitro studies reported that ELF-MF could affect antioxidant activities [Wolf et al, 2005;Polaniak et al, 2010], cell growth [Wolf et al, 2005;Sulpizio et al, 2011], and cytoskeletal organization [Sulpizio et al, 2011] and could induce DNA damage through the action of free radical species [Wolf et al, 2005;Vijayalaxmi and Prihoda, 2009;Bułdak et al, 2012;Luukkonen et al, 2014]. Recently, several papers evaluated positive effects of ELF-MF on cytokine profiles driving transition from a chronic pro-inflammatory state to an anti-inflammatory state associated with the healing process [Costin et al, 2012;Pesce et al, 2013;Saliev et al, 2014] and neurodegenerative diseases [Mattsson and Simk o, 2012].…”

Section: Introductionmentioning

confidence: 99%

Effect of extremely low frequency magnetic fields on cell proliferation and gene expression

Lee

Hong

Jung

et al. 2015

Bioelectromagnetics

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Owing to concerns regarding possible effects of extremely low frequency magnetic fields (ELF‐MF) on human health, many studies have been conducted to elucidate whether ELF‐MF can induce modifications in biological processes. Despite this, controversies regarding effects of ELF‐MF are still rife. In this study, we investigated biological effects of ELF‐MF on MCF10A, MCF7, Jurkat, and NIH3T3 cell lines. ELF‐MF with a magnetic flux density of 1 mT at 60 Hz was employed to stimulate cells for 4 or 16 h, after which the effects of ELF‐MF on cell proliferation, cell death, cell viability, and DNA synthesis rates were assessed. Whereas Jurkat and NIH3T3 cells showed no consistent variation in cell number, cell viability, and DNA synthesis rate, MCF10A and MCF7 cells showed consistent and significant decreases in cell number, cell viability, and DNA synthesis rates. However, there was no effect of ELF‐MF on cell death in any of tested cell lines. Next, to investigate the effect of ELF‐MF on gene expression, we exposed MCF7 cells to 2 mT at 60 Hz for 16 h and examined transcriptional responses by using gene expression array. We found a gene, PMAIP1, that exhibited statistically significant variation using two‐fold cut‐off criteria and certified its expression change by using semi‐quantitative and quantitative reverse transcription polymerase chain reaction. From these results, we concluded that ELF‐MF could induce the delay of cell cycle progression in MCF7 and MCF10A cells in a cell context‐specific manner and could up‐regulate PMAIP1 in MCF7 cells. Bioelectromagnetics. 36:506–516, 2015. © 2015 Wiley Periodicals, Inc.

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Short‐term exposure to 50 Hz ELF‐EMF alters the cisplatin‐induced oxidative response in AT478 murine squamous cell carcinoma cells

Cited by 65 publications

References 39 publications

Pu-erh Tea Powder Preventive Effects on Cisplatin-Induced Liver Oxidative Damage in Wistar Rats

Pu-erh Tea Powder Preventive Effects on Cisplatin-Induced Liver Oxidative Damage in Wistar Rats

Effect of extremely low‐frequency electromagnetic fields on antioxidant activity in the human keratinocyte cell line NCTC 2544

Effect of extremely low frequency magnetic fields on cell proliferation and gene expression

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