Short-Term High Fructose Intake Impairs Diurnal Oscillations in the Murine Cornea

He, Jingxin; Jiao, Xinwei; Sun, Xin; Huang, Yijia; Xu, Pengyang; Xue, Yunxia; Fu, Ting; Li, Zhijie

doi:10.1167/iovs.62.10.22

Cited by 21 publications

(7 citation statements)

References 98 publications

(126 reference statements)

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“…A change in the inflammatory markers could be attributed to the altered clock, as fructose treatment led to reduced daily levels of Clock , Per1 , and Cry1 mRNA, and FruFA led to lower levels of CLOCK and CRY1 protein. These results are supported by previously published reports [ 14 , 31 , 32 ]. Bmal1 expression is positively upregulated by RORα and negatively regulated by REV-ERBα [ 3 , 5 ].…”

Section: Discussionsupporting

confidence: 93%

Differential Effect of Fructose in the Presence or Absence of Fatty Acids on Circadian Metabolism in Hepatocytes

2023

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We aimed to explore whether fructose in the absence or presence of fatty acids modulates circadian metabolism in AML-12 hepatocytes. Fructose treatment under steatosis conditions (FruFA) led to fat synthesis resulting in increased triglycerides and cholesterol content. Fructose led to reduced activity of the AMPK and mTOR-signaling pathway. However, FruFA treatment led to inhibition of the AMPK signaling pathway but activation of the mTOR pathway. Fructose also increased the expression of inflammatory markers, whereas the addition of fatty acids dampened their circadian expression. At the clock level, fructose or FruFA altered the expression of the core clock. More specifically, fructose led to altered expression of the BMAL1-RORα-REV-ERBα axis, together with reduced phosphorylated BMAL1 levels. In conclusion, our results show that hepatocytes treated with fructose respond differently if fatty acids are present, leading to a differential effect on metabolism and circadian rhythms. This is achieved by modulating BMAL1 activity and expression.

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Section: Discussionsupporting

confidence: 93%

Differential Effect of Fructose in the Presence or Absence of Fatty Acids on Circadian Metabolism in Hepatocytes

2023

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“…The present study provides another dimensional analysis of the pattern of altered physiological activity of MGs due to excessive lipid intake. Consistent with previously studied ocular tissues, such as the cornea (11) and lacrimal gland (26), nutritional challenges dramatically altered the output transcriptome of circadian rhythms in both gene composition and the oscillations of their enriched signaling pathways. MGs play an important role in maintaining the stability of the tear film mainly through the lipid layer of the tear film (3,58,59).…”

Section: Discussionsupporting

confidence: 78%

“…This stability is not only present in metabolic stress but also in aging organs and tissues (51)(52)(53). Similarly, the same stabilization phenomenon has been observed in ocular tissues subjected to nutritional challenges, such as the cornea (11) and the lacrimal gland (26) subjected to high fructose intake and the lacrimal gland subjected to a high-fat diet (26). Similarly, this asynchrony is present in the nutritionally challenged liver (54), as well as in several aging tissues (51)(52)(53).…”

Section: Discussionmentioning

confidence: 60%

“…Similarly, ocular tissues and their physiological activities undergo synchronous rhythmic changes ( 10 ). Published studies, including our team’s series of work, suggest that the cornea ( 11 – 13 ), lacrimal gland ( 14 , 15 ), retinal pigment epithelium, and retina ( 16 , 17 ) all exhibit robust rhythmic changes in the phase of the lighting cycle. However, little attention has been paid so far to the circadian rhythmical pattern of MGs and their underlying mechanisms ( 18 , 19 ).…”

Section: Introductionmentioning

confidence: 99%

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High-fat intake reshapes the circadian transcriptome profile and metabolism in murine meibomian glands

Zou

Liu

et al. 2023

Front. Nutr.

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BackgroundNutritional and food components reshape the peripheral clock and metabolism. However, whether food challenges affect the circadian clock and metabolism of meibomian glands (MGs) has not been fully explored. This study was designed to analyze alterations in the rhythmic transcriptome and metabolism of MGs of murine fed a balanced diet or a high-fat diet (HFD).MethodsMale C57BL/6J mice were maintained on a 12/12 h light/dark cycle and fed ad libitum on normal chow (NC) or HFD for 4 weeks. MGs were collected from sacrificed animals at 3-h intervals throughout a 24-h circadian cycle. The circadian transcriptome of MGs was analyzed via bioinformatics approaches using high-throughput RNA sequencing (RNA-seq). In addition, circadian oscillations of lipid components in MGs were analyzed.ResultsMeibomian glands displayed robust transcriptome rhythmicity. HFD feeding significantly altered the circadian transcriptome profile of MGs—including composition and phase—and spatiotemporally affected the enriched signaling pathways. In addition, HFD feeding significantly altered the normal rhythmic oscillations of lipid components in MGs.ConclusionOur data show that HFD significantly affects MGs’ rhythmicity, which reveals a high sensitivity of MGs’ clocks to lipid composition in food.

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“…These findings underscore the complex interplay between the host and its commensal microbiota. In addition, previous studies have demonstrated significant circadian rhythmicity in critical ocular components, such as the lacrimal gland, which is responsible for tear film secretion, and the cornea, which is located at the front of the eye (Jiao et al, 2019;Jiao et al, 2020;He et al, 2021;Jiao et al, 2021). However, whether the microbiota populating the ocular surface exhibit analogous rhythmic oscillations remains an unresolved question that warrants further investigation.…”

Section: Introductionmentioning

confidence: 99%

Temporal dynamics and composition of ocular surface microbiota in C57BL/6J mice: uncovering a 12h ultradian rhythm

Jiao,

2023

Front. Cell. Infect. Microbiol.

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PurposeThis study aimed to investigate the presence of rhythmic fluctuations in the composition, abundance, and functions of commensal core bacteria on the ocular surface of C57BL/6J mice.MethodsMale C57BL/6J mice, aged 12 weeks, were subjected to a 12-hour light/12-hour dark cycle. Ocular surface tissue samples were collected at four time points (ZT) over a 24-hour period at six-hour intervals. The core ocular surface microbiota’s oscillation cycles and frequencies were assessed using 16S rRNA gene sequencing targeting the V3-V4 region, along with the JTK_CYCLE algorithm. Functional predictions of these bacteria were conducted using PICRUSt2.ResultsDeep sequencing of the ocular surface microbiota highlighted the high abundance of commensal bacteria, with Proteobacteria, Actinobacteriota, and Firmicutes collectively constituting over 90% of the total sample abundance. Among the 22 core bacterial genera, 11 exhibited robust 12-hour rhythms, including Halomonas, Pelagibacterium, Pseudomonas, Nesterenkonia, norank_f_Hyphomonadaceae, Stenotrophomonas, Anoxybacillus, Acinetobacter, Zoogloea, Brevibacillus, and Ralstonia. Further taxonomic analysis indicated significant intra-cluster similarities and inter-cluster differences at the order, family, and genus levels during ZT0/12 and ZT6/18. Community interaction networks and functional prediction analyses revealed synchronized 12-hour rhythmic oscillations in neural, immune, metabolic, and other pathways associated with symbiotic bacteria.ConclusionThis study demonstrates the presence of ultradian rhythmic oscillations in commensal bacteria on the ocular surface of normal C57BL/6J mice, with a 12-hour cycle. These findings suggest a crucial role for ultradian rhythms in maintaining ocular surface homeostasis in the host.

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Short-Term High Fructose Intake Impairs Diurnal Oscillations in the Murine Cornea

Cited by 21 publications

References 98 publications

Differential Effect of Fructose in the Presence or Absence of Fatty Acids on Circadian Metabolism in Hepatocytes

Differential Effect of Fructose in the Presence or Absence of Fatty Acids on Circadian Metabolism in Hepatocytes

High-fat intake reshapes the circadian transcriptome profile and metabolism in murine meibomian glands

Temporal dynamics and composition of ocular surface microbiota in C57BL/6J mice: uncovering a 12h ultradian rhythm

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