Sialic acid cyclization of human Th homing receptor glycan associated with recurrent exacerbations of atopic dermatitis

Sakuma, Keiichiro; Furuhashi, Takuya; Kondo, Sachiko; Yabe, Uichiro; Ohmori, Katsuyuki; Ito, Hidemi; Aoki, Masahiro; Morita, Akimichi; Kannagi, Reiji

doi:10.1016/j.jdermsci.2012.09.015

Cited by 3 publications

(3 citation statements)

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“…Both serum total IgE and eosinophil count are important clinical parameters of asthma. Cyclic sialyl 6-sulfo Le X is synthesised from sialyl 6-sulfo Le X by sialic-acid-cyclizing-enzymes, such as sialic acid de-N-acetylase and cyclase; cyclic sialyl 6-sulfo LeX could also be reconverted back to sialyl 6-sulfo Le X by acid-decyclizing-enzymes, such as sialic acid hydrolase and N-acetyltransferase 17 . Altered activities of sialyl acid cyclizing and decyclizing enzymes in memory helper T cells might be a possible explanation behind our observation, and would be explored in our future study.…”

Section: Discussionmentioning

confidence: 99%

“…After two washes with PBS(-), the cells were incubated with FITC-conjugated secondary antibodies for 30 minutes at 4 °C. After hemolysis with FACS lysing solution (BD Biosciences) and two washes with PBS(-), the cells were subjected to flow cytometry analysis as described previously 17 .…”

Section: Methodsmentioning

confidence: 99%

“…The antibodies used included allophycocyanin (APC)-cyanin (Cy)7- conjugated anti-CD4 (RPA-T4), peridinin chlorophyll protein (PerCP)-Cy5.5-conjugated anti-CD25 (M-A251), phycoerythrin(PE)-Cy7-conjugated anti-CD45RO (UCHL1), Brilliant Violet 421-conjugated anti-CCR4 (L291H4), PE-conjugated anti-CCR7 (G043H7), FITC-conjugated anti-mouse IgG1 (PMG1-1), FITC-conjugated anti-mouse IgM (RMM-1) (BioLegend, San Diego, CA, USA), anti-human CD15s (CSLEX1(RUO)) (BD Pharmingen, San Jose, CA, USA), anti-sialyl 6-sulfo Le x (G152, mouse IgM) 17 , and anti-cyclic sialyl 6-sulfo Le x (G159, mouse IgG1) 17 . Leukocytes were treated with the FOXP3 Fix/Perm buffer set (BioLegend) to increase the permeability of cells, before being stained with Alexa Fluor 647-conjugated anti-FOXP3 (259D) (BioLegend).…”

Section: Methodsmentioning

confidence: 99%

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Sialyl Glycan Expression on T Cell Subsets in Asthma: a correlation with disease severity and blood parameters

Yeh

Kannagi

et al. 2019

Sci Rep

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Memory T helper (Th) and regulatory T (Treg) cells play key roles in asthma. Certain sialyl carbohydrate determinants for selectins profoundly affect the migratory properties of memory Th cells, and the suppressive function of Treg cells. Previous studies have shown that the proportion of CCR4 + memory Th cells expressing sialyl 6-sulfo Lewis X (Le X ) is elevated in asthma patients. We aim to investigate the roles of different sialyl glycans on T cell subsets in asthma. Using flow cytometry, we assessed the expression of three sialyl glycans, sialyl 6-sulfo Le X , cyclic sialyl 6-sulfo Le X , and sialyl Le X on memory Th and Treg cells, in the peripheral blood of asthmatic children. We also assessed the relationships between glycan-expressing cell percentages and asthma clinical parameters. Compared with controls, asthmatic children showed higher proportions of memory Th cells expressing sialyl Le X and sialyl 6-sulfo Le X . The proportions of memory Th cells with sialyl 6-sulfo Le X and cyclic sialyl 6-sulfo Le X expression in asthmatic children correlated with absolute eosinophil count and IgE level, respectively. Children with moderate-to-severe asthma had lower numbers of sialyl Le X positive Treg cells. Our study suggests that sialyl glycans on T cells may play important roles in the pathogenesis of asthma.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%