Sialidase Production and Genetic Diversity in Clostridium perfringens Type A Isolated from Chicken with Necrotic Enteritis in Brazil

Llanco, Luis; Nakano, Viviane; Avila-Campos, Mario Júlio

doi:10.1007/s00284-014-0722-5

Cited by 11 publications

(5 citation statements)

References 28 publications

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“…In contrast with our findings, Chukwu et al [19] found 44.7% of the C. perfringens isolates were typeable using PCR-based genotyping with arbitrary OPA-3 primers. Llanco et al [46] found all (100%) of the C. perfringens isolates typeable, which is in agreement with our findings. Due to fact the raw meat samples were non-outbreak related and that random and different types of samples were analyzed [47], a relatively wide genetic diversity among the C. perfringens isolates was expected in this study.…”

Section: Discussionsupporting

confidence: 93%

Prevalence, Antibiotic Resistance, Toxin-Typing and Genotyping of Clostridium perfringens in Raw Beef Meats Obtained from Qazvin City, Iran

Hassani

Pakbin

Brück³

et al. 2022

Antibiotics

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Background: Clostridium perfringens is one of the highest prevailing spore-forming foodborne pathogens, which is widely distributed and causes severe disease and outbreaks in humans and animals. Raw meat and poultry are the main vehicles of this pathogen. In this study, we investigated the prevalence, antibiotic resistance pattern, toxin-encoding genes and genetic diversity of C. perfringens isolates from raw whole and minced meat samples purchased from local markets in Qazvin city, Iran (the source of beef cattle production was also located in Qazvin city, Iran). Methods: We used conventional culture-based and Kirby–Bauer disk diffusion and conventional and arbitrary primer PCR methods. Results: A total of 18 C. perfringens strains were isolated from 133 raw meat samples (13.53%). Up to 44.4 and 55.5% of these isolates were detected in raw minced and whole meat samples, respectively. We found that 72.2, 66.6, 61.1, 37.8 and 33.3% of the C. perfringens isolates were resistant to ampicillin, tetracycline, amoxicillin, ciprofloxacin and chloramphenicol antibiotics, respectively. Multidrug resistance was found in 38% of the isolates. Among the four main toxin genes evaluated, the Cpa gene was detected in all isolates, and 61.1% of the isolates were mostly recognized as type A C. perfringens. High levels of genetic diversity were observed among the isolates, and they were classified into five distinct groups. Conclusions: The isolates from whole meat samples were more resistant to antibiotics. However, toxin genes were more detected in the isolates from minced meat samples. Our findings suggest that contamination of raw meat products with multidrug resistant C. perfringens could be regarded as one of the concerning pathogens in these products. Comprehensive monitoring of C. perfringens isolates is strongly recommended.

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Section: Discussionsupporting

confidence: 93%

Prevalence, Antibiotic Resistance, Toxin-Typing and Genotyping of Clostridium perfringens in Raw Beef Meats Obtained from Qazvin City, Iran

Hassani

Pakbin

Brück³

et al. 2022

Antibiotics

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“…Three sialidases, encoded by genes nanH, nanI and nanJ in C. perfringens , are also named as neuraminidases or exo-sialidases (NanI and NanJ). This group of enzymes represent important virulence factors during C. perfringens -mediated tissue infection; they catalyse hydrolysis of terminal sialic acids from glycoprotein, glycolipids and polysaccharides of cell membranes that aids in bacterial attachment to host cells 71 . This mucolytic potential suggests that C. perfringens may utilise intestinal mucus as a nutrient source, and thereby potentiates intestinal colonisation, which has been modelled using the in vitro Caco-2 cell line 72 .…”

Section: Disease Initiationsmentioning

confidence: 99%

An update on the human and animal enteric pathogen Clostridium perfringens

Kiu

Hall

2018

Emerging Microbes & Infections

337

285

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Clostridium perfringens, a rapid-growing pathogen known to secrete an arsenal of >20 virulent toxins, has been associated with intestinal diseases in both animals and humans throughout the past century. Recent advances in genomic analysis and experimental systems make it timely to re-visit this clinically and veterinary important pathogen. This Review will summarise our understanding of the genomics and virulence-linked factors, including antimicrobial potentials and secreted toxins of this gut pathogen, and then its up-to-date clinical epidemiology and biological role in the pathogenesis of several important human and animal-associated intestinal diseases, including pre-term necrotising enterocolitis. Finally, we highlight some of the important unresolved questions in relation to C. perfringens-mediated infections, and implications for future research directions.

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“…All strains showed a conserved DNA sequence revealing an identity of 100%, except for the strain JF3703 for which the observed differences were located in this domain (RYCHENER et al, 2017). Sialidase activity of C. perfringens type A has previously been detected by LLANCO et al (2014), where the nanH, nanI, and nanJ genes were investigated and different patterns were observed, indicating heterogeneity of the microorganism. However, C. chauvoei, unlike a bacterial enteric pathogen, is highly specialized.…”

Section: Resultsmentioning

confidence: 92%

Molecular characterization of virulence genes cctA, nanA, and fliC in Clostridium chauvoei from Rio Grande do Sul and São Paulo State, Brazil

et al. 2019

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Clostridium chauvoei toxin A (CctA), neuraminidase (NanA), and flagellin (FliC) proteins contribute to the pathogenicity of Clostridium chauvoei, the causative agent of blackleg in cattle. The aim of this study was to analyze the genetic variability of cctA, nanA, and fliC genes in C. chauvoei isolates from the Rio Grande do Sul and São Paulo state- Brazil, during different sampling periods. The presence of these genes was verified through PCR amplification and partial gene sequencing of 17 strains. Alignment of PCR amplicons combined with bioinformatics analysis was used in an attempt to study the variability across C. chauvoei solates. The similarity among the partial sequences of cctA and nanA genes was 100%. The sequencing of fliC revealed three different paralog alleles of flagellin, and two strains were seen to be polymorphic, with amino acid alterations in the predicted protein. Overall, this study indicates that strains of C. chauvoei isolated in Brazil are highly conserved with respect to the virulence factors evaluated.

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Sialidase Production and Genetic Diversity in Clostridium perfringens Type A Isolated from Chicken with Necrotic Enteritis in Brazil

Cited by 11 publications

References 28 publications

Prevalence, Antibiotic Resistance, Toxin-Typing and Genotyping of Clostridium perfringens in Raw Beef Meats Obtained from Qazvin City, Iran

Prevalence, Antibiotic Resistance, Toxin-Typing and Genotyping of Clostridium perfringens in Raw Beef Meats Obtained from Qazvin City, Iran

An update on the human and animal enteric pathogen Clostridium perfringens

Molecular characterization of virulence genes cctA, nanA, and fliC in Clostridium chauvoei from Rio Grande do Sul and São Paulo State, Brazil

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