Siderophores of <i>Bacillus pumilus</i> promote 2‐keto‐L‐gulonic acid production in a vitamin C microbial fermentation system

Zhang, Qian; Lin, Ying; Shen, Guozheng; Zhang, Haihong; Lyu, Shuxia

doi:10.1002/jobm.202200237

Cited by 16 publications

(16 citation statements)

References 44 publications

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“…Bacillus pumilus is a Gram-positive, spore-forming bacteria, which commonly occurs in various environments including marine water, deep-sea sediments, and soil ( Priest, 1993 ; Shivaji et al, 2006 ; Liu et al, 2013 ; Pudova et al, 2022 ; Yakovleva et al, 2022 ; Zhang et al, 2022 ). This species exhibits significant resistance to environmental stresses, e.g., low or no nutrient availability, drought, irradiation, UV radiation, chemical disinfectants, or oxidizing enzymes ( Nicholson et al, 2000 ).…”

Section: Introductionmentioning

confidence: 99%

Potential of Bacillus pumilus to directly promote plant growth

2022

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Plant Growth-Promoting Bacteria (PGPB) are a promising alternative to conventional fertilization. One of the most interesting PGPB strains, among the spore-forming bacteria of the phylum Firmicutes, is Bacillus pumilus. It is a bacterial species that inhabits a wide range of environments and shows resistance to abiotic stresses. So far, several PGPB strains of B. pumilus have been described, including B. pumilus LZP02, B. pumilus JPVS11, B. pumilus TUAT-1, B. pumilus TRS-3, and B. pumilus EU927414. These strains have been shown to produce a wide range of phytohormones and other plant growth-promoting substances. Therefore, they can affect various plant properties, including biometric traits, substance content (amino acids, proteins, fatty acids), and oxidative enzymes. Importantly, based on a study with B. pumilus WP8, it can be concluded that this bacterial species stimulates plant growth when the native microbiota of the inoculated soil is altered. However, there is still a lack of research with deeper insights into the structure of the native microbial community (after B. pumilus application), which would provide a better understanding of the functioning of this bacterial species in the soil and thus increase its effectiveness in promoting plant growth.

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Section: Introductionmentioning

confidence: 99%

Potential of Bacillus pumilus to directly promote plant growth

2022

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“…vulgare and 2-KGA production. Lyu et al , found that addition of the supernatant of the associated bacteria in the middle and late stages of the fermentation period, that is, the period of spore formation and release, can greatly promote the accumulation of 2-KGA. A comparison of the growth of B.…”

Section: Characteristics Of Key Strains Employed In the Classic Two-s...mentioning

confidence: 98%

“…Following the maturation and release of spores, a large number of active substances, such as purines, amino acids, fatty acids, and vitamin B 1 , are released into the medium, which can promote the growth of K. vulgare and 2-KGA production. Lyu et al 52,53 found that addition of the supernatant of the associated bacteria in the middle and late stages of the fermentation period, that is, the period of spore formation and release, can greatly promote the accumulation of 2-KGA. A comparison of the growth of B. endophyticus ST-1 in single or mixed culture with K. vulgare 25-B-1 by Lyu et al 54,55 revealed a shortened growth period of K. vulgare 25-B-1 and faster growth of the associated bacteria in the mixed-culture system.…”

Section: The Interaction Between Associated Bacteria and K Vulgarementioning

confidence: 99%

Recent Advances in 2-Keto-l-gulonic Acid Production Using Mixed-Culture Fermentation and Future Prospects

Liu,

Chen

et al. 2024

J. Agric. Food Chem.

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Vitamin C, also known as ascorbic acid, is an essential vitamin that cannot be synthesized by the human body and must be acquired through our diet. At present, the precursor of vitamin C, 2-keto-L-gulonic acid (2-KGA), is typically produced via a two-step fermentation process utilizing three bacterial strains. The second step of this traditional two-step fermentation method involves mixed-culture fermentation employing 2-KGA-producing bacteria (Ketogulonicigenium vulgare) along with associated bacteria. Because K. vulgare has defects in various metabolic pathways, associated bacteria are needed to provide key substances to promote K. vulgare growth and 2-KGA production. Unlike previous reviews where the main focus was the interaction between associated bacteria and K. vulgare, this Review presents the latest scientific research from the perspective of the metabolic pathways associated with 2-KGA production by K. vulgare and the mechanism underlying the interaction between K. vulgare and the associated bacteria. In addition, the dehydrogenases that are responsible for 2-KGA production, the 2-KGA synthesis pathway, strategies for simplifying 2-KGA production via a one-step fermentation route, and, finally, future prospects and research goals in vitamin C production are also presented.

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“…K. vulgare is responsible for producing 2-keto-L-gulonic acid (2-KLG, the precursor of vitamin C) from L-sorbose via L-sorbosone but with an extremely low yield. Bacillus strains (e.g., Bacillus megaterium , Bacillus endophyticus , Bacillus subtilis , Bacillus pumilus ), as helper strains, are cocultured to significantly stimulate the growth of K. vulgare and 2-KLG yields ( Yakushi et al, 2020 ; Zeng et al, 2020 ; Fang et al, 2021 ; Yang et al, 2021 ; Zhang et al, 2022 ). The relationship between K. vulgare and the helper strain has been one of the hot topics focused on in the field of vitamin C fermentation.…”

Section: Introductionmentioning

confidence: 99%

iTRAQ-based proteomics analysis of Bacillus pumilus responses to acid stress and quorum sensing in a vitamin C fermentation system

Zhang

Lyu

2023

Front. Microbiol.

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Microbial consortia play a key role in human health, bioenergy, and food manufacturing due to their strong stability, robustness and versatility. One of the microbial consortia consisting of Ketogulonicigenium vulgare and Bacillus megaterium for the production of the vitamin C precursor, 2-keto-L-gulonic acid (2-KLG), has been widely used for large-scale industrial production. To further investigate the cell–cell communication in microbial consortia, a microbial consortium consisting of Ketogulonicigenium vulgare and Bacillus pumilus was constructed and the differences in protein expression at different fermentation time points (18 h and 40 h) were analyzed by iTRAQ-based proteomics. The results indicated that B. pumilus was subjected to acid shocks in the coculture fermentation system and responded to it. In addition, the quorum sensing system existed in the coculture fermentation system, and B. pumilus could secrete quorum-quenching lactonase (YtnP) to inhibit the signaling pathway of K. vulgare. This study offers valuable guidance for further studies of synthetic microbial consortia.

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Siderophores of Bacillus pumilus promote 2‐keto‐L‐gulonic acid production in a vitamin C microbial fermentation system

Cited by 16 publications

References 44 publications

Potential of Bacillus pumilus to directly promote plant growth

Potential of Bacillus pumilus to directly promote plant growth

Recent Advances in 2-Keto-l-gulonic Acid Production Using Mixed-Culture Fermentation and Future Prospects

iTRAQ-based proteomics analysis of Bacillus pumilus responses to acid stress and quorum sensing in a vitamin C fermentation system

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