Significance of the coenzyme Q system in the classification of yeasts and yeast-like organisms. XLIX. The phylogenetic relationships of the Q6-equipped species in the teleomorphic apiculate yeast genera Hanseniaspora, Nadsonia, and Saccharomycodes based on the partial sequences of 18S and 26S ribosomal ribonucleic acids.

Abstract

Eleven strains of species in the apiculate yeast genera Hanseniaspora, Nadsonia, and Saccharomycodes were examined for partial base sequence determinations of 18S and 26S rRNAs. In the partial base sequence of 26S rRNA (positions 493-622, 130 bases), percent similarities were 65-79, 76-84, and 70-76 between the genera Hanseniaspora and Nadsonia, the genera Hanseniaspora and Saccharomycodes, and the genera Nadsonia and Saccharomycodes, respectively. These apiculate yeasts showed 72-85 percent similarity with S. cerevisiae. In the partial base sequences of 26S rRNA (positions 1611-1835, 225 bases) and of 18S rRNA (positions 1451-1618, 168 bases), the number of base differences was calculated to be 38-26, 27-9, and 30-23, and 12-8, 11-5, and 13-8 between the above-mentioned genera, respectively. These apiculate yeasts showed 33-9, and 12-4 base differences, respectively, with S. cerevisiae. The three apiculate yeast genera were recognized phylogenetically based on the sequence data obtained. Some discussions were made, especially on dividing the members of the genus Hanseniaspora into two groups at the generic level.The teleomorphic species of apiculate yeasts equipped with coenzyme Q-6 (Q-6 or Q6) are currently classified in the following three genera: Hanseniaspora Zikes, t Significance of the coenzyme Q system in the classification of yeasts and yeast-like organisms .

Phylogeny of the Yeast Genera Hanseniaspora (Anamorph Kloeckera), Dekkera (Anamorph Brettanomyces), and Eeniella as Inferred from Partial 26S Ribosomal DNA Nucleotide Sequences

“…Only Kloeckera antillarum IF0 0669 (= CBS 2578) (H. occidentalis) was found to have antigens unique to the genus Kloeckeraspora. Because of the lack of correlation between phenotypes, as well as the weak statistical support of the subclades observed in the phylogram of the genus Hanseniaspora, we do not accept the proposal of Yamada et al (33,34) to reinstate the genus Kloeckeraspora.…”

“…However, spheroidal and ledged ascospores, which are not released at maturity, occur in H. uvarum. The genus Kloeckeraspora differs from the genus Hanseniaspora principally by the presence of spheroidal, warty ascospores, which are not released at maturity (33,34), although smooth spheroidal ascospores with equatorial ledges may be present in H. occidentalis as well.…”

“…The genus Hanseniaspora is characterized by apiculate cells with percurrent, bipolar budding (23) and a coenzyme Q which contains six isoprene units (33,34). Species are defined on the basis of levels of nuclear DNA relatedness (14), and the species have been supported by the results of partial 18s and 26s rRNA nucleotide sequence analyses (33,34).…”

“…Species are defined on the basis of levels of nuclear DNA relatedness (14), and the species have been supported by the results of partial 18s and 26s rRNA nucleotide sequence analyses (33,34). However, the genus Hanseniaspora is heterogeneous with respect to ascospore morphology (23), serology (28), and proton magnetic resonance spectra of cell wall mannans (26).…”

Hanseniaspora nectarophila sp. nov., a yeast species isolated from ephemeral flowers

“…This classification has been confirmed by several phylogenetic studies (Yamada et al, 1992;Boekhout et al, 1994;Kurtzman, 2003), although a single phylogenetic marker that can reliably reconstruct the relationships between species of the genus Hanseniaspora has not yet been described (Cadez et al, 2006). As the rates for nucleotide substitutions in the LSU D1/D2 region between closely related species of Hanseniaspora are lower than between the majority of yeast species (Kurtzman & Robnett, 1998), a polyphasic approach for species delineation must be applied.…”