Significantly Accelerated Wound Healing of Full-Thickness Skin Using a Novel Composite Gel of Porcine Acellular Dermal Matrix and Human Peripheral Blood Cells

Abstract

Here we report the fabrication of a novel composite gel from decellularized gal-gal-knockout porcine skin and human peripheral blood mononuclear cells (hPBMCs) for full-thickness skin wound healing. Decellularized skin extracellular matrix (ECM) powder was prepared via chemical treatment, freeze drying, and homogenization. The powder was mixed with culture medium containing hyaluronic acid to generate a pig skin gel (PSG). The effect of the gel in regeneration of full-thickness wounds was studied in nude mice. We found significantly accelerated wound closure already on day 15 in animals treated with PSG only or PSG + hPBMCs compared to untreated and hyaluronic acid-treated controls (p < 0.05). Addition of the hPBMCs to the gel resulted in marked increase of host blood vessels as well as the presence of human blood vessels. At day 25, histologically, the wounds in animals treated with PSG only or PSG + hPBMCs were completely closed compared to those of controls. Thus, the gel facilitated generation of new skin with well-arranged epidermal cells and restored bilayer structure of the epidermis and dermis. These results suggest that porcine skin ECM gel together with human cells may be a novel and promising biomaterial for medical applications especially for patients with acute and chronic skin wounds.

New Nanotechnologies for the Treatment and Repair of Skin Burns Infections

“…Kuna et al produced a novel composite gel from human peripheral blood mononuclear cells (hPBMCs) and decellularized gal-gal-knockout porcine skin for skin wound healing [103]. To obtain a pig skin gel (PSG), the prepared decellularized skin extracellular matrix (ECM) powder was mixed with culture medium containing hyaluronic acid.…”

The in vivo effect of Lacto-N-neotetraose (LNnT) on the expression of type 2 immune response involved genes in the wound healing process

“…Full-thickness wound is one of the most common type of skin damages. Delayed wound healing process in this skin lesion may lead to some serious consequences including post-injury infections and hypertrophic scars 22,23 . Therefore, researchers are trying to apply different strategies to accelerate the repair process and prevent these consequences.…”

Skin Tissue Substitutes and Biomaterial Risk Assessment and Testing

“…The use of immunomodulatory biomaterials in an implant localizes immunosuppression to the wound site by removing the need for immunosuppressive drugs while having the potential to further reduce poor cosmetic outcomes. Common strategies in immunomodulation for skin regeneration include macrophage polarization (Sun, 2017 ; Castellano et al, 2018 ), the use of glycosaminoglycans (GAGs) (Bhowmick et al, 2017 ; Pezeshki-Modaress et al, 2017 ), and the use of decellularized matrices (Kuna et al, 2017 ).…”

“…The major benefit of using decellularized ECM as a tissue scaffold is that the ECM inherently has a set of biomolecules that are naturally involved in the wound healing process. For example, decellularized pig skin was prepared as a gel with hyaluronic acid (HA) (Kuna et al, 2017 ). The gel, which contained 66.6, 3.5, and 4.6 μg/mL of collagen, elastin, and GAGs, respectively, showed a marked improvement in wound healing in nude mice by promoting rapid infiltration of host cells and improved wound stabilization.…”