Silencing long noncoding RNA OGFRP1 inhibits the proliferation and migration of cervical carcinoma cells

Zou, Kun; Yu, Haifeng; Chen, Xuehua; Ma, Qiang; Hou, Lifang

doi:10.1002/cbf.3435

Cited by 12 publications

(9 citation statements)

References 29 publications

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“…An increasing number of lncRNAs are identified to play a vital role in tumorigenesis and progression. Except for the lncRNAs that have been studied in CC, such as HOXD-AS1 [ 25 ], TUG1 [ 26 ], MEG3 [ 27 ] and OGFRP1 [ 28 ], there is still a long way to go to identify and characterize new lncRNAs for control of CC initiation and progression. More importantly, LINC00441 was upregulated in CC tissue samples compared with normal tissues.…”

Section: Discussionmentioning

confidence: 99%

LINC00441 promotes cervical cancer progression by modulating miR-450b-5p/RAB10 axis

Han¹,

Shao

Liu³

2020

Cancer Cell Int

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Background As one of the most common gynaecological malignant tumors, cervical cancer (CC) has become an important public health issue. Emerging evidence has revealed long non-coding RNAs (lncRNAs) are crucial regulators of biological functions in cancers, including CC. And the oncogenic role of LINC00441 has been verified in hepatocellular carcinoma (HCC). But the molecular mechanism and biological functions of LINC00441 in CC remain unknown. Methods qRT-PCR analysis detected the expression of genes in CC tissues or cells. CCK-8, colony formation, flow cytometry, transwell, western blot assays as well as animal studies were conducted to analyze the function of LINC00441 in CC. Luciferase reporter, RIP and RNA pull down assays were applied to verify the binding relations among the indicated genes. Results LINC00441 was upregulated in CC tissues and cells. Further, LINC00441 depletion repressed cell proliferation and motility in vitro as well as tumor growth in vivo. LINC00441 could sponge miR-450b-5p to upregulate RAB10 expression. Finally, miR-450b-5p inhibitor or RAB10 upregulation counteracted LINC00441 knockdown-mediated function on the development of CC. Conclusions LINC00441 drives CC progression by targeting miR-450b-5p/RAB10 axis, which might provide new idea for researching CC-related molecular mechanism.

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Section: Discussionmentioning

confidence: 99%

LINC00441 promotes cervical cancer progression by modulating miR-450b-5p/RAB10 axis

Han¹,

Shao

Liu³

2020

Cancer Cell Int

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“…Homo sapiens opioid growth factor receptor pseudogene 1 (OGFRP1), with 1201 nucleotides in length, is a recently identified lncRNA located on chromosome 22q13.2. OGFRP1 is found to be up-regulated in endometrial cancer [ 22 ] and cervical carcinoma [ 23 ]. Furthermore, OGFRP1 suppression inhibits the malignant behavior of the endometrial cancer cells (Ishikawa) [ 22 ], hepatocellular carcinoma cells (Hep3B) [ 24 ], cervical carcinoma cells (C33A and SiHa) [ 23 ], gestational choriocarcinoma cells (JEG3) [ 25 ] and human coronary artery endothelial cells (HCAECs) [ 26 ].…”

Section: Introductionmentioning

confidence: 99%

LncRNA OGFRP1 acts as an oncogene in NSCLC via miR-4640-5p/eIF5A axis

Liu

Niu

et al. 2021

Cancer Cell Int

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Background Long noncoding RNAs (lncRNAs) OGFRP1 is up-regulated in endometrial cancer and cervical carcinoma, and OGFRP1 suppression inhibits the malignant behavior of cancer cells. Here, we evaluated the expression pattern, biological function and potential mechanism of OGFRP1 in non-small cell lung cancer (NSCLC). Methods The expression of target genes in 25 pairs of clinically collected NSCLC and normal lung tissue samples was detected by qRT-PCR or western blot. We screened the siRNA (siOGFRP1) to down-regulate the expression of OGFRP1 in A549 and H1299 cells. The biological function of A549 and H1299 cells were examined by CCK8, wound healing and transwell assays. The molecular mechanism of OGFRP1 was further explored. Results The expression of OGFRP1 in NSCLC tissues were higher than that in normal lung tissue. siOGFRP1 inhibited the proliferation, migration and invasion of A549 and H1299 cells. In addition, the expression of EMT-related and apoptosis-related proteins was changed by siOGFRP1 transfection. OGFRP1 can directly interact with miR-4640-5p, and siOGFRP1 increased the level of miR-4640-5p. Moreover, miR-4640-5p could directly bind to the 3’ UTR region of eIF5A mRNA. eIF5A was highly expressed in NSCLC tissues, and predicted a poor prognosis. In addition, the expression of miR-4640-5p and eIF5A in NSCLC tissues were negatively correlated, while the expression of OGFRP1 and eIF5A were positively correlated. Knockdown of OGFRP1 inhibited the expression of eIF5A, while transfection of miR-4640-5p inhibitor up-regulated the expression of eIF5A. Conclusions Taken together, we demonstrated that down-regulation of OGFRP1 inhibited the progression of NSCLC through miR-4640-5p/eIF5A axis.

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“…These data revealed the oncogene function of OGFRP1 in NSCLC, which was consistent with the ndings in endometrial cancer, [22] hepatocellular carcinoma, [24] gestational choriocarcinoma cells (JEG3) [25] and cervical carcinoma cells. [23] Eukaryotic translation initiation factor 5A (eIF5A) is an 18-kDa protein that participates in mRNA-related functions, such as transcription, [35,36] mRNA turnover [37] and nucleoplasmic transport, [38] plays a role in the initiation and extension of protein synthesis, [39,40] which is essential for cell proliferation. Vertebrates carry two genes, which encode two highly homologous eIF5A subtypes, namely eIF5A1 and eIF5A2.…”

Section: Discussionmentioning

confidence: 99%

“…OGFRP1 is found to be up-regulated in endometrial cancer [22] and cervical carcinoma. [23] Furthermore, OGFRP1 suppression inhibits the malignant behaviour (inhibits cell viability, promotes apoptosis, and suppresses cell migration and invasion) of the endometrial cancer cells (Ishikawa), [22] hepatocellular carcinoma cells (Hep3B), [24] cervical carcinoma cells (C33A and SiHa) [23], gestational choriocarcinoma cells (JEG3) [25] and human coronary artery endothelial cells (HCAECs). [26] However, the expression pattern, biological function and potential mechanism of OGFRP1 in NSCLC have not been investigated.…”

Section: Introductionmentioning

confidence: 99%

LncRNA OGFRP1 acts as an oncogene in NSCLC via miR-4640-5p/eIF5A axis

Liu

Zhai

Xiao

et al. 2020

Preprint

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Background Long noncoding RNAs (lncRNAs) OGFRP1 is up-regulated in endometrial cancer and cervical carcinoma, and OGFRP1 suppression inhibits the malignant behaviour of cancer cells. However, the role of OGFRP1 in non-small-cell lung cancer (NSCLC) have not been investigated. Here, we evaluated the expression pattern, biological function and potential mechanism of OGFRP1 in NSCLC. Methods We screened the siRNA (siOGFRP1) to down-regulate the expression of OGFRP1 in A549 and H1299 cells. The biological function of A549 and H1299 cells were examined by CCK8, wound healing and transwell assays. The molecular mechanism of OGFRP1 was further explored. Results siOGFRP1 significantly inhibited the cell proliferation, migration and invasion of A549 and H1299 cells. In addition, the expression of EMT-related and apoptosis-related proteins was changed by siOGFRP1 transfection. MiR-4640-5p could directly bind to the 3’ UTR region of eIF5A1. Moreover, OGFRP1 bound to miR-4640-5p through the same binding site, which facilitated the expression of eIF5A1. eIF5A1 overexpression rescued cell proliferation, migration and invasion inhibition induced by OGFRP1 down-regulation and miR-4640-5p up-regulation in A549 and H1299 cells. Conclusions Taken together, we demonstrated that down-regulation of OGFRP1 inhibited the progression of NSCLC through miR-4640-5p/eIF5A1 axis.

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Silencing long noncoding RNA OGFRP1 inhibits the proliferation and migration of cervical carcinoma cells

Cited by 12 publications

References 29 publications

LINC00441 promotes cervical cancer progression by modulating miR-450b-5p/RAB10 axis

LINC00441 promotes cervical cancer progression by modulating miR-450b-5p/RAB10 axis

LncRNA OGFRP1 acts as an oncogene in NSCLC via miR-4640-5p/eIF5A axis

LncRNA OGFRP1 acts as an oncogene in NSCLC via miR-4640-5p/eIF5A axis

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