Silencing of copine genes confers common wheat enhanced resistance to powdery mildew

Zou, Baohong; Yuan, Ding; Li, He; Hua, Jian

doi:10.1111/mpp.12617

Cited by 20 publications

(14 citation statements)

References 32 publications

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“…The field trials in this study support some investigations conducted in controlled conditions where plant responses to obligate pathogens were either independent of resistance genes and determined with temperature changes 25 or influenced by calcium-dependent phospholipid-binding proteins responsive to both pathogen infection and temperature changes 26 . The results of this study showed that in agro-ecological conditions in Serbia, high temperatures in February could initiate a predominance of yellow rust in a greater number of winter wheat genotypes compared to the number of genotypes in the year when high winter temperatures are met only in January.…”

Section: Discussionsupporting

confidence: 78%

Diversity in susceptibility reactions of winter wheat genotypes to obligate pathogens under fluctuating climatic conditions

Jevtić

Župunski

Lalošević

et al. 2020

Sci Rep

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To date, studies have usually focused on the impact of abiotic factors on the distribution of plant pathogens and have built forecast models for the prediction of pathogen outbreaks. However, the impact of the combined effects of biotic and abiotic factors on the prevalence of economically important pathogens has usually been neglected. The objective of this study was to determine the relationship between powdery mildew and rusts of wheat and to examine how the combined effects of abiotic and biotic factors influence their prevalence. The study was conducted in the period 2016–2019 using the collection of 2158 genotypes of winter wheat. The most influential factors on disease indices and relationships among obligate pathogens were determined using multiple regression models and principal component analysis. The possibility of the coexistence of different rust species in the same growing season and in the same field was shown. The significant influence of fluctuations in winter temperatures on changes in the prevalence of obligate pathogens was determined. The strong impact of genotypes and their reaction on climatic elements in certain phenological stages were shown to be significant factors influencing the interactions among obligate pathogens and the predominance of one pathogen over another.

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Section: Discussionsupporting

confidence: 78%

Diversity in susceptibility reactions of winter wheat genotypes to obligate pathogens under fluctuating climatic conditions

Jevtić

Župunski

Lalošević

et al. 2020

Sci Rep

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“…These results indicate that TaPT29-6A silencing weakens resistance to powdery mildew caused by Bgt . To examine whether silencing TaPHT1 could affect the expression levels of defense response genes, TaPR4A/B [45,46] and TaPR2/10 [72,73] were chosen as marker genes for defense responses to necrotrophic and biotrophic pathogens. In addition, qRT-PCR analysis showed that transcript levels of the TaPR4A/B and TaPR2/10 marker genes for defense responses to necrotrophic and biotrophic pathogens were significantly lower in the TaPT29-6A -silenced plants than in the controls (Figure 9D), in accordance with the susceptible phenotypes of VIGS plants.…”

Section: Resultsmentioning

confidence: 99%

Integrative Analysis of the Wheat PHT1 Gene Family Reveals A Novel Member Involved in Arbuscular Mycorrhizal Phosphate Transport and Immunity

Zhang

et al. 2019

Cells

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Phosphorus (P) deficiency is one of the main growth-limiting factors for plants. However, arbuscular mycorrhizal (AM) symbiosis can significantly promote P uptake. Generally, PHT1 transporters play key roles in plants’ P uptake, and thus, PHT1 genes have been investigated in some plants, but the regulation and functions of these genes in wheat (TaPHT1) during AM symbiosis have not been studied in depth. Therefore, a comprehensive analysis of TaPHT1 genes was performed, including sequence, phylogeny, cis-elements, expression, subcellular localization and functions, to elucidate their roles in AM-associated phosphate transport and immunity. In total, 35 TaPHT1s were identified in the latest high-quality bread wheat genome, 34 of which were unevenly distributed on 13 chromosomes, and divided into five groups. Sequence analysis indicated that there are 11 types of motif architectures and five types of exon-intron structures in the TaPHT1 family. Duplication mode analysis indicated that the TaPHT1 family has expanded mainly through segmental and tandem duplication events, and that all duplicated gene pairs have been under purifying selection. Transcription analysis of the 35 TaPHT1s revealed that not only known the mycorrhizal-specific genes TaPht-myc, TaPT15-4B (TaPT11) and TaPT19-4D (TaPT10), but also four novel mycorrhizal-specific/inducible genes (TaPT3-2D, TaPT11-4A, TaPT29-6A, and TaPT31-7A) are highly up-regulated in AM wheat roots. Furthermore, the mycorrhizal-specific/inducible genes are significantly induced in wheat roots at different stages of infection by colonizing fungi. Transient Agrobacterium tumefaciens-mediated transformation expression in onion epidermal cells showed that TaPT29-6A is a membrane-localized protein. In contrast to other AM-specific/inducible PHT1 genes, TaPT29-6A is apparently required for the symbiotic and direct Pi pathway. TaPT29-6A-silenced lines exhibited reduced levels of AM fungal colonization and arbuscules, but increased susceptibility to biotrophic, hemi-biotrophic and necrotrophic pathogens. In conclusion, TaPT29-6A was not only essential for the AM symbiosis, but also played vital roles in immunity.

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“…Common wheat provides dietary carbohydrates for more than one-third of the population in the world ( Zou et al, 2018 ). Production of wheat is critical for global food security, but its grain yield and quality are negatively affected by various pathogens.…”

Section: Introductionmentioning

confidence: 99%

Silencing of the Wheat Protein Phosphatase 2A Catalytic Subunit TaPP2Ac Enhances Host Resistance to the Necrotrophic Pathogen Rhizoctonia cerealis

Zhu

Wang

et al. 2018

Front. Plant Sci.

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Eukaryotic type 2A protein phosphatases (protein phosphatase 2A, PP2A) consist of a scaffold subunit A, a regulatory subunit B, and a catalytic subunit C. Little is known about the roles of PP2Ac proteins that are involved in plant responses to necrotrophic fungal pathogens. Sharp eyespot, caused by the necrotrophic fungus Rhizoctonia cerealis, is a destructive disease of wheat (Triticum aestivum), an important staple food crop. Here, we isolated TaPP2Ac-4D from wheat, which encodes a catalytic subunit of the heterotrimeric PP2A, and characterized its properties and role in plant defense response to R. cerealis. Based on the sequence alignment of TaPP2Ac-4D with the draft sequences of wheat chromosomes from the International Wheat Genome Sequencing Consortium (IWGSC), it was found that TaPP2Ac-4D gene is located on the long arm of the wheat chromosome 4D and has two homologs assigned on wheat chromosomes 4A and 4B. Sequence and phylogenetic tree analyses revealed that the TaPP2Ac protein is a typical member of the PP2Ac family and belongs to the subfamily II. TaPP2Ac-4B and TaPP2Ac-4D displayed higher transcriptional levels in the R. cerealis-susceptible wheat cultivar Wenmai 6 than those seen in the resistant wheat line CI12633. The transcriptional levels of TaPP2Ac-4B and TaPP2Ac-4D were significantly elevated in wheat R. cerealis after infection and upon H2O2 treatment. Virus-induced gene silencing results revealed that the transcriptional knockdown of TaPP2Ac-4D and TaPP2Ac-4B significantly increased wheat resistance to R. cerealis infection. Meanwhile, the transcriptional levels of certain pathogenesis-related (PR) and reactive oxygen species (ROS)-scavenging enzyme encoding genes were increased in TaPP2Ac-silenced wheat plants. These results suggest that TaPP2Ac-4B and TaPP2Ac-4D negatively regulate defense response to R. cerealis infection possibly through modulation of the expression of certain PR and ROS-scavenging enzyme genes in wheat. This study reveals a novel function of the plant PP2Ac genes in plant immune responses.

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Silencing of copine genes confers common wheat enhanced resistance to powdery mildew

Cited by 20 publications

References 32 publications

Diversity in susceptibility reactions of winter wheat genotypes to obligate pathogens under fluctuating climatic conditions

Diversity in susceptibility reactions of winter wheat genotypes to obligate pathogens under fluctuating climatic conditions

Integrative Analysis of the Wheat PHT1 Gene Family Reveals A Novel Member Involved in Arbuscular Mycorrhizal Phosphate Transport and Immunity

Silencing of the Wheat Protein Phosphatase 2A Catalytic Subunit TaPP2Ac Enhances Host Resistance to the Necrotrophic Pathogen Rhizoctonia cerealis

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