Silencing of JHEH and EcR genes of Plutella xylostella (Lepidoptera: Plutellidae) through double stranded RNA oral delivery

Chaitanya, B.; Asokan, R.; Sita, Timothy L.; Rebijith, K. B.; Kumar, Priti; Kumar, N. K. Krishna

doi:10.1016/j.aspen.2017.03.020

Cited by 20 publications

(20 citation statements)

References 77 publications

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“…In insect pest control, RNAi-mediated gene knockdown effect has been demonstrated in Diptera, Coleoptera, Hymenoptera, Orthoptera, Blattodea, Lepidoptera, and Hemiptera [ 18 , 19 , 20 , 21 ]. Among Lepidoptera, satisfactory RNAi results have been reported in Spodoptera exigua (H.) (Noctuidae) [ 22 ] Helicoverpa armigera (H.) (Noctuidae) [ 23 ] Plutella xylostella (L.) (Plutellidae) [ 24 ], and Mythimna separata (W.) (Noctuidae) [ 25 ]. Regarding T. absoluta , functional RNAi was achieved in several genes such as Juvenile hormone inducible protein ( JHP ), Juvenile hormone epoxide hydrolase protein ( JHEH ), Ecdysteroid 25-hydroxylase ( PHM ), Chitin synthase A ( CHI ), Carboxylesterase ( COE ) [ 11 ], Arginine kinase ( AK ) [ 10 , 11 ], and Vacuolar ATPase-A, which exhibited significant larval mortality in this pest [ 11 , 26 ].…”

Section: Introductionmentioning

confidence: 99%

dsRNA-Mediated Pest Management of Tuta absoluta Is Compatible with Its Biological Control Agent Nesidiocoris tenuis

Sarmah

Kaldis

Taning

et al. 2021

Insects

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RNAi-mediated insect pest management has recently shown promising results against the most serious pest of tomato, the tomato leafminer, Tuta absoluta. This study aimed to investigate whether dsRNA (dsTa-αCOP) designed to target the T. absoluta-αCOP gene could cause adverse effects to its biocontrol agent, the mirid predator, Nesidiocoris tenuis. Oral exposure of N. tenuis to dsRNA (dsNt-αCOP) designed to target N. tenuis-αCOP resulted in a 61%, 67% and 55% reduction in its transcript level in comparison to the sucrose, dsGFP and dsTa-αCOP treatments, respectively. In addition, significantly higher mortality of 57% was recorded in dsNt-αCOP-treated N. tenuis when compared to the sucrose (7%), dsGFP (10%) and dsTa-αCOP (10%) treatments. Moreover, the predation rate of ~33–39 Ephestia kuehniella eggs per N. tenuis adult dramatically reduced to almost half in the surviving dsNt-αCOP-treated N. tenuis. This worst-case exposure scenario confirmed for the first time that the RNAi machinery is functional in this species and that the risk of exposure through the oral route is possible. In contrast, dsTa-αCOP did not cause any sub-lethal effects to N. tenuis upon oral exposure. Oral exposure of T. absoluta to dsTa-αCOP resulted in 50% mortality. In the context of a biosafety risk assessment of RNAi-mediated insect management, investigating the effects on non-target organisms is essential in order to include this method as part of an integrated pest management strategy. Based on our laboratory assays, RNAi-mediated control is compatible with the biological control of T. absoluta by its natural enemy N. tenuis, adding the RNAi approach in the armoire of integrated pest management of T. absoluta.

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Section: Introductionmentioning

confidence: 99%

dsRNA-Mediated Pest Management of Tuta absoluta Is Compatible with Its Biological Control Agent Nesidiocoris tenuis

Sarmah

Kaldis

Taning

et al. 2021

Insects

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“…Finding a suitable gene as a target, as well as a proper delivery method, is crucial for successful RNAi technology (Majidiani et al, 2019). The oral assay of in vitro synthesized dsRNA could be effective in several insect species, such as Diabrotica virgifera LeConte (Baum et al, 2007), Bemisia tabaci (Gennadius; Upadhyay et al, 2011), Plutella xylostella (L.) (Chaitanya et al, 2017), and Mythimna separata Walker (Ganbaatar et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

A gene silencing of V‐ATPase subunit A interferes with survival and development of the tomato leafminer, Tuta absoluta

Rahmani

Bandani

2020

Arch Insect Biochem Physiol

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RNA interference (RNAi) technology is not only considered as a tool to analyze gene function, but it is also potentially considered as a strategy to develop novel biopesticide. In the current study, a double-stranded RNA specific to v-ATPase subunit A of the tomato leafminer, Tuta absoluta (Meyrick; Lepidoptera: Gelechiidae), was orally administered. A gradual decrease in the expression of the gene was observed from Day 1 to 3 and resulted in significant larval mortality. These results suggest that v-ATPases A can be considered as a promising target gene by RNAi technology to be used in the management of the tomato leafminer.

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“…The siRNAs were supplied to the larvae by oral delivery as reported elsewhere (Chaitanya et al, 2017;Gong et al, 2011), with some modifications. Each siRNA was spread onto cabbage leaf discs (Brassica oleracea) and fed to 4 th -instar larvae.…”

Section: Sirna Preparationmentioning

confidence: 99%

A gustatory receptor tuned to the steroid plant hormone brassinolide inPlutella xylostella(Lepidoptera: Plutellidae)

Yang

Gong

et al. 2020

Preprint

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Feeding and oviposition deterrents help phytophagous insects to identify host plants. The taste organs of phytophagous insects contain bitter gustatory receptors (GRs). To explore their function, we focused on PxylGr34, a bitter GR in Plutella xylostella (L.). We detected abundant PxylGr34 transcripts in the larval head and specific expression of PxylGr34 in the antennae of females. Analyses using the Xenopus oocyte expression system and two-electrode voltage-clamp recording showed that PxylGr34 is specifically tuned to the plant hormone brassinolide (BL) and its analog 24-epibrassinolide. Electrophysiological analyses revealed that the medial sensilla styloconica on the maxillary galea of the 4th instar larvae are responsive to BL. Dual-choice bioassays demonstrated that BL inhibits larval feeding and female ovipositing. Knock-down of PxylGr34 by RNAi abolished BL-induced feeding inhibition. These results shed light on gustatory coding mechanisms and deterrence of insect feeding and ovipositing, and may be useful for designing plant hormone-based pest management strategies.

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Silencing of JHEH and EcR genes of Plutella xylostella (Lepidoptera: Plutellidae) through double stranded RNA oral delivery

Cited by 20 publications

References 77 publications

dsRNA-Mediated Pest Management of Tuta absoluta Is Compatible with Its Biological Control Agent Nesidiocoris tenuis

dsRNA-Mediated Pest Management of Tuta absoluta Is Compatible with Its Biological Control Agent Nesidiocoris tenuis

A gene silencing of V‐ATPase subunit A interferes with survival and development of the tomato leafminer, Tuta absoluta

A gustatory receptor tuned to the steroid plant hormone brassinolide inPlutella xylostella(Lepidoptera: Plutellidae)

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