Silicon enhances the growth of Phaeodactylum tricornutum Bohlin under green light and low temperature

Zhao, Peipei; Gu, Wenhui; Wu, Songcui; Huang, Aiyou; He, Linwen; Xie, Xiujun; Gao, Shan; Zhang, Baoyu; Niu, Jianfeng; Lin, Apeng; Wang, Guangce

doi:10.1038/srep03958

Cited by 44 publications

(31 citation statements)

References 57 publications

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“…Microscopic analyses (Figure 4c,d) revealed a high occurrence of fusiform cells, a limited number of triradiate shapes, and no appearance of the oval form in all of our growth conditions. It has been reported that the fusiform type is dominant in suspension culture over the other forms [43,50], which is in agreement with our tested conditions. It has also been reported that the triradiate P. tricornutum cells are rarely found in laboratory growth conditions [1,43].…”

Section: Visual Analysis Of P Tricornutum Cell Morphology and Lipid supporting

confidence: 93%

Red Light Variation an Effective Alternative to Regulate Biomass and Lipid Profiles in Phaeodactylum tricornutum

et al. 2020

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Marine water diatom Phaeodactylum tricornutum is a photosynthetic organism that is known to respond to the changing light environment and adapt to different temperatures to prevent photoinhibition and maintain its metabolic functions. The objective of the present study was to test whether light shift variations in different growth phases impact the growth and lipid metabolism of P. tricornutum. Thus, we investigated R exposure in different growth phases to find the most effective light shift condition. The results showed that substituting white light (W) by red light (R) under autotrophic conditions, a condition called red shift (RS), increased biomass and lipid content compared to levels found under continuous W or R exposure alone. We observed an increase by 2-fold biomass and 2.3-fold lipid content in RS as compared to W. No significant change was observed in the morphology of lipid droplets, but the fatty acid (FA) composition was altered. Specifically, polyunsaturated FAs were increased, whereas monounsaturated FAs decreased in P. tricornutum grown in RS compared to W control. Therefore, we propose that a light shift during the beginning of the stationary phase is a low-cost cultivation strategy to boost the total biomass and lipids in P. tricornutum.Appl. Sci. 2020, 10, 2531 2 of 18 for the high production of desired bioactive molecules. Therefore, light should be provided with specific spectral components, intensity, and duration. Multiple ecophysiological studies have been published reporting on the distinct behaviors of different diatoms and algae in response to variations in light [4,5]. It has been shown that light quality has an impact on chloroplast migration and on the light acclimation reactions of photosynthesis [6,7]. Red light has been linked to stimulating growth, ethylene production [8], lipid accumulation [7], and increasing thylakoid stacking [6]. have suggested the involvement of phytochromes in increasing the intracellular nitrate content on exposure to red light for short periods altering the overall cellular nitrate content. Recent discoveries in genomics have revealed exciting information on phytochromes, blue-light sensing cryptochromes, and aureochromes [10]. In a study by Jungandreas et al. [11], it has been reported that P. tricornutum cells acclimated to both red and blue light have comparable metabolite profiles, but drastic changes in metabolites and carbon partitioning was observed under red to blue light shift. In addition, various interdisciplinary studies have shown that light perceived by the red-light receptor (phytochromes) can regulate nutrient metabolism, cellular events, and signaling cascades [12]. These discoveries are still in progress and much remains unknown, but these studies can be explored for application-based diatom experiments such as developing low-cost cultivation technology or efficient bioreactors.In both culture medium and natural water bodies, light (intensity, distribution, photoperiod) may vary and will not behave similar to the dissolved salts or nu...

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Section: Visual Analysis Of P Tricornutum Cell Morphology and Lipid supporting

confidence: 93%

Red Light Variation an Effective Alternative to Regulate Biomass and Lipid Profiles in Phaeodactylum tricornutum

et al. 2020

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“…Various factors, including nutritional and physical, exert positive effects on P . tricornutum growth (Yongmanitchai and Ward, 1991;Zhao et al, 2014). Biomass, storage lipids, and saturated and monounsaturated fatty acids of P .…”

Section: Biomass Production and Growth Ratementioning

confidence: 99%

A comparative analysis of fatty acid composition and fucoxanthin content in six Phaeodactylum tricornutum strains from diff erent origins

Wang

et al. 2015

Chin. J. Ocean. Limnol.

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Phaeodactylum tricornutum is a potential livestock for the combined production of eicosapentaenoic acid (EPA) and fucoxanthin. In this study, six marine diatom strains identifi ed as P . tricornutum were cultured and their total lipid, fatty acid composition and major photosynthetic pigments determined. It was found that the cell dry weight concentration and mean growth rate ranged between 0.24-0.36 g/L and 0.31-0.33/d, respectively. Among the strains, SCSIO771 presented the highest total lipid content, followed by SCSIO828, and the prominent fatty acids in all strains were C16:0, C16:1, C18:1, and C20:5 (EPA). Polyunsaturated fatty acids, including C16:2, C18:2, and EPA, comprised a signifi cant proportion of the total fatty acids. EPA was markedly high in all strains, with the highest in SCSIO828 at 25.65% of total fatty acids. Fucoxanthin was the most abundant pigment in all strains, with the highest in SCSIO828 as well, at 5.50 mg/g. The collective results suggested that strain SCSIO828 could be considered a good candidate for the concurrent production of EPA and fucoxanthin.

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“…However, although silicon does not seem to be required for laboratory or factory cultures of Phaeodactylum tricornutum, it seems to be needed for normal expression of miRNAs and growth [419].…”

Section: Furthermore Rnai Knock-down Of Nitrate Reductase Can Enhancmentioning

confidence: 99%

The lipid biochemistry of eukaryotic algae

Li‐Beisson

Thelen

Fedosejevs

et al. 2019

Progress in Lipid Research

308

249

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Silicon enhances the growth of Phaeodactylum tricornutum Bohlin under green light and low temperature

Cited by 44 publications

References 57 publications

Red Light Variation an Effective Alternative to Regulate Biomass and Lipid Profiles in Phaeodactylum tricornutum

Red Light Variation an Effective Alternative to Regulate Biomass and Lipid Profiles in Phaeodactylum tricornutum

A comparative analysis of fatty acid composition and fucoxanthin content in six Phaeodactylum tricornutum strains from diff erent origins

The lipid biochemistry of eukaryotic algae

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