Silk Biomaterials with Vascularization Capacity

Han, Heyou; Hongyan, Ning; Liu, Shanshan; Lü, Qiang; Fan, Zhihai; Lei, Hao; Lu, Guozhong; Kaplan, David L.

doi:10.1002/adfm.201504160

Cited by 100 publications

(184 citation statements)

References 60 publications

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“…Han et al prepared water-insoluble SF scaffolds containing physical cues instead of growth factors for vascularization. The SF-based scaffolds could improve cell differentiation into endothelial cells and promote neovascularization, and eliminate many inherent disadvantages caused by growth factors at the same time [94]. …”

Section: Structure Design Of Silk Fibroin-based Biomaterialsmentioning

confidence: 99%

A Review of Structure Construction of Silk Fibroin Biomaterials from Single Structures to Multi-Level Structures

Wang

Wei

et al. 2017

IJMS

404

241

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The biological performance of artificial biomaterials is closely related to their structure characteristics. Cell adhesion, migration, proliferation, and differentiation are all strongly affected by the different scale structures of biomaterials. Silk fibroin (SF), extracted mainly from silkworms, has become a popular biomaterial due to its excellent biocompatibility, exceptional mechanical properties, tunable degradation, ease of processing, and sufficient supply. As a material with excellent processability, SF can be processed into various forms with different structures, including particulate, fiber, film, and three-dimensional (3D) porous scaffolds. This review discusses and summarizes the various constructions of SF-based materials, from single structures to multi-level structures, and their applications. In combination with single structures, new techniques for creating special multi-level structures of SF-based materials, such as micropatterning and 3D-printing, are also briefly addressed.

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Section: Structure Design Of Silk Fibroin-based Biomaterialsmentioning

confidence: 99%

A Review of Structure Construction of Silk Fibroin Biomaterials from Single Structures to Multi-Level Structures

Wang

Wei

et al. 2017

IJMS

404

241

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“…Then the DNA spectra were collected at an excitation wavelength of 480 nm and an emission wavelength of 530 nm using a spectrofluorometer (BioTek Synergy 4 BioTek, Winooski, VT, Canada) and the DNA concentration was calculated based on the standard curves. 55 …”

Section: Methodsmentioning

confidence: 99%

Simulation of ECM with silk and chitosan nanocomposite materials

Ding

et al. 2017

J. Mater. Chem. B

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Extracellular matrix (ECM) is a system used to model the design of biomaterial matrices for tissue regeneration. Various biomaterial systems have been developed to mimic the composition or microstructure of the ECM. However, emulating multiple facets of the ECM in these systems remains a challenge. Here, a new strategy is reported which addresses this need by using silk fibroin and chitosan (CS) nanocomposite materials. Silk fibroin was first assembled into ECM-mimetic nanofibers in water and then blended with CS to introduce the nanostructural cues. Then the ratios of silk fibroin and CS were optimized to imitate the protein and glycosaminoglycan compositions. These biomaterial scaffolds had suitable compositions, hierarchical nano-to-micro structures, and appropriate mechanical properties to promote cell proliferation in vitro, and vascularization and tissue regeneration in vivo. Compared to previous silk-based scaffolds, these scaffolds achieved improvements in biocompatibility, suggesting promising applications in the future in tissue regeneration.

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“…Silk-based degradable scaffolds biomaterials showed remarkable vascularization capacity and are used in soft tissue engineering and regenerative medicine. 75 These biomaterials are mainly noncrystalline, offering improved cell proliferation than previously reported silk materials. These systems also have appropriate softer mechanical property that could provide physical cues to promote cell differentiation into endothelial cells, and enhance neo vascularization and tissue in growth in vivo without the addition of growth factors.…”

Section: Tissue Vascularization and Integrationmentioning

confidence: 99%

“…More-specifically endogenous cellular factors contribute a lot to tissue engineering and repair but cells also efficiently utilize exogenous sources to substitute for the organ's lost structure and/or function(s). The immobilization or delivery of growth factors has been explored to improve vascularization capacity of tissue engineered constructs; however, the use of growth factors has inherent problems such as the loss of signaling capability and the risk of complications such as immunological responses and cancer 75 (Table 2). Several cytokines, chemokines, and growth factors maintain micro-and niche play crucial roles in HPC activation and differentiation.…”

Section: Growth Factorsmentioning

confidence: 99%

“…These systems also have appropriate softer mechanical property that could provide physical cues to promote cell differentiation into endothelial cells, and enhance neo vascularization and tissue in growth in vivo without the addition of growth factors. 75 Alginate scaffolds provide a favorable microenvironment for liver neo-tissue recreation and regeneration. Tissue engineering has some pervasive problems i.e.…”

Section: Tissue Vascularization and Integrationmentioning

confidence: 99%

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Role of Biological Scaffolds, Hydro Gels and Stem Cells in Tissue Regeneration Therapy

Upadhyay¹

2017

ATROA

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Present review article emphasizes role of biological scaffolds, hydrogels and stem cells in tissue engineering mainly in regeneration or repairing of damaged tissues. Highly porous scaffold biomaterials are developed which act as templates for tissue regeneration and potentially guide the growth of new tissue. Present article also describes de-cellularization, cell printing, vascularization, integration of cross linking of methods for scaffolding of biomaterials to reproduce and recapitulate complexity in engineered tissues. It also explains different scaffold types, polymer hydrogels which are necessary for formation of microstructure, cell attachment, differentiation, tissue vascularization and integration. It also explains use of induced pluripotent stem cells (iPSCs) and engineered MSCs as new diagnostic and potential therapeutic tools to remove sustained damage and complications from organ failures. These engineered MSCs assist in making self-assembling supramolecular hydrogels, which have larger applications in cell therapy of intractable diseases and tissue regeneration. No doubt development of more advanced biomaterials, growth factors and stem cell derived products/factors and tissue transplantation methods based on cell regeneration programming will revolutionize the clinical therapeutics. This article suggests identification of new biomaterials/biomolecules such as growth factors, scaffolds, integration, adhesion and regulatory molecules and cell secreted factors which can induce major metabolic and signaling pathways during phase of tissue repairing and induction of regeneration. This innovative research area needs many conceptual improvements in organ therapies, methods and technological advancement to scale more services to the human society.

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Silk Biomaterials with Vascularization Capacity

Cited by 100 publications

References 60 publications

A Review of Structure Construction of Silk Fibroin Biomaterials from Single Structures to Multi-Level Structures

A Review of Structure Construction of Silk Fibroin Biomaterials from Single Structures to Multi-Level Structures

Simulation of ECM with silk and chitosan nanocomposite materials

Role of Biological Scaffolds, Hydro Gels and Stem Cells in Tissue Regeneration Therapy

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