Silk scaffolds connected with different naturally occurring biomaterials for prostate cancer cell cultivation in 3<scp>D</scp>

Bäcker, Anne; Erhardt, Olga; Wietbrock, Lukas; Schel, Natalia; Göppert, Bettina; Dirschka, M.; Abaffy, Paul; Sollich, Thomas; Cecilia, A.; Gruhl, Friederike J.

doi:10.1002/bip.22993

Cited by 28 publications

(19 citation statements)

References 25 publications

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“…3D porous scaffolds also promote the formation of bigger spheroids, compared to non-porous scaffolds, and enhance tumor cell invasion and therapeutic resistance [83,84]. Although they are synthesized mainly by polymers such as poly(ε-caprolactone), porous microparticles can consist of different substances, including natural (e.g., chitosan, hyaluronic acid, alginate, collagen, gelatin, silk fibroin), and synthetic (e.g., poly(lactide-co-glycolide)) materials [85][86][87][88][89][90]. The porosity and pore size of the scaffold are essential for the establishment of effective 3D models, as they can affect the transport of oxygen, metabolites and nutrients, as well as cell adhesion and cell growth [91].…”

Section: Scaffold-based Techniquesmentioning

confidence: 99%

Three-Dimensional Spheroids as In Vitro Preclinical Models for Cancer Research

et al. 2020

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Most cancer biologists still rely on conventional two-dimensional (2D) monolayer culture techniques to test in vitro anti-tumor drugs prior to in vivo testing. However, the vast majority of promising preclinical drugs have no or weak efficacy in real patients with tumors, thereby delaying the discovery of successful therapeutics. This is because 2D culture lacks cell–cell contacts and natural tumor microenvironment, important in tumor signaling and drug response, thereby resulting in a reduced malignant phenotype compared to the real tumor. In this sense, three-dimensional (3D) cultures of cancer cells that better recapitulate in vivo cell environments emerged as scientifically accurate and low cost cancer models for preclinical screening and testing of new drug candidates before moving to expensive and time-consuming animal models. Here, we provide a comprehensive overview of 3D tumor systems and highlight the strategies for spheroid construction and evaluation tools of targeted therapies, focusing on their applicability in cancer research. Examples of the applicability of 3D culture for the evaluation of the therapeutic efficacy of nanomedicines are discussed.

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Section: Scaffold-based Techniquesmentioning

confidence: 99%

Three-Dimensional Spheroids as In Vitro Preclinical Models for Cancer Research

et al. 2020

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“…Therefore, the cell microenvironment has to mimic in vivo physiological conditions including spatial and temporal dimensions and dynamics, physical interactions, such as cell–cell contact, and the cell–extracellular matrix . Several technologies are currently available to produce such microtissues with good control of the dimensions and that exhibit tissue‐like phenotypes including porous scaffolds and polymers, hydrogels and ultralow attachment cell culture plates . These methods take advantage of the natural self‐assembly tendency that is typical of several cell types.…”

Section: Introductionmentioning

confidence: 99%

Bioluminescence Imaging of Spheroids for High‐throughput Longitudinal Studies on 3D Cell Culture Models

Cevenini

Calabretta

Lopreside

et al. 2017

Photochem & Photobiology

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Bioluminescent (BL) cell-based assays based on two-dimensional (2D) monolayer cell cultures represent well-established bioanalytical tools for preclinical screening of drugs. However, cells in 2D cultures do not often reflect the morphology and functionality of living organisms, thus limiting the predictive value of 2D cell-based assays. Conversely, 3D cell models have the capability to generate the extracellular matrix and restore cell-to-cell communications; thus, they are the most suitable model to mimic in vivo physiology. In this work, we developed a nondestructive real-time BL imaging assay of spheroids for longitudinal studies on 3D cell models. A high-throughput BL 3D cell-based assay in micropatterned 96-well plate format is reported. The assay performance was assessed using the transcriptional regulation of nuclear factor K beta response element in human embryonic kidney (HEK293) cells. We compared concentration-response curves for tumor necrosis factor-α with those obtained using conventional 2D cell cultures. One of the main advantages of this approach is the nonlysing nature of the assay, which allows for repetitive measurements on the same sample. The assay can be implemented in any laboratory equipped with basic cell culture facilities and paves the way to the development of new 3D bioluminescent cell-based assays.

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“…Previously, SF/Cs (1: 1) was proven to be a potential biomaterial for generating scaffolds for cancer therapy [ 23 ]. Coincidentally, a recent study suggested that the SF/Cs (1: 1) scaffolds can improve cell growth as a 3D cell culture platform for prostate cancer research compared with matrices based on pure biomaterials or synthetic polymers [ 24 ]. However, the results from our preliminary experiment have shown that the SF/Cs (1: 1) scaffold has poor water absorption as well as low degradation and cell adhesion rates, thereby restricting their applications in the 3D cell culture.…”

Section: Discussionmentioning

confidence: 99%

Preparation and Characterization of a Novel Triple Composite Scaffold Containing Silk Fiborin, Chitosan, and Alginate for 3D Culture of Colonic Carcinoma Cells In Vitro

Liang

Han

et al. 2020

Med Sci Monit

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Background Three-dimensional (3D) cell-culture scaffolds are ideal in vitro models to bridge the gap between two-dimensional cell culture in vitro and in vivo cancer models. Construction of 3D scaffolds using two kinds of biomaterials has been reported, but there are still many defects. To improve the performance of the scaffolds for 3D cell culture of colonic carcinoma (CC) cells in vitro , we attempted to construct triple composite scaffolds using silk fibroin (SF), chitosan (Cs), and alginate (Alg). Material/Methods We explored the suitability of triple composite scaffolds of SF/Cs/Alg at ratios of 1: 1: 0.5, 1: 1: 1, and 1: 1: 2 for 3D culture of CC cells, and used the dual composite scaffold of SF/Cs (1: 1) as a control group. We analyzed the physicochemical characteristics of these scaffolds and studied cell adhesion, cell proliferation, migration, colony-forming ability, microstructure and ultrastructure, and spheroid-forming capacity of the commercially available CC cell line HCT-116 on the prepared scaffolds. Results Our results show that SF/Cs/Alg (1: 1: 1) scaffolds demonstrated the best profile, the highest uniform porosity and connectivity, and excellent hydroscopicity, and also exhibited appropriate and controlled swelling and degradation characteristics. The adhesion, proliferation, colony-forming, and wound-healing assays, green fluorescent protein-labeled HCT116 cell imaging, 4′,6-diamidino-2-phenylindole and DY-554-phalloidin staining, scanning electron microscopy, and haematoxylin and eosin staining revealed that the triple composite scaffolds of SF/CS/Alg (1: 1: 1) supported cell adhesion, proliferation, migration, colony-forming ability, and spheroid formation far better than the dual composite scaffold of SF/CS (1: 1). Conclusions This study successfully demonstrated the potential of SF/Cs/Alg (1: 1: 1) scaffold as an alternative for the 3D in vitro culture of CC cells.

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Silk scaffolds connected with different naturally occurring biomaterials for prostate cancer cell cultivation in 3D

Cited by 28 publications

References 25 publications

Three-Dimensional Spheroids as In Vitro Preclinical Models for Cancer Research

Three-Dimensional Spheroids as In Vitro Preclinical Models for Cancer Research

Bioluminescence Imaging of Spheroids for High‐throughput Longitudinal Studies on 3D Cell Culture Models

Preparation and Characterization of a Novel Triple Composite Scaffold Containing Silk Fiborin, Chitosan, and Alginate for 3D Culture of Colonic Carcinoma Cells In Vitro

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