Silver Colloid Nanoparticles:  Synthesis, Characterization, and Their Antibacterial Activity

Abstract

A one-step simple synthesis of silver colloid nanoparticles with controllable sizes is presented. In this synthesis, reduction of [Ag(NH(3))(2)](+) complex cation by four saccharides was performed. Four saccharides were used: two monosaccharides (glucose and galactose) and two disaccharides (maltose and lactose). The syntheses performed at various ammonia concentrations (0.005-0.20 mol L(-1)) and pH conditions (11.5-13.0) produced a wide range of particle sizes (25-450 nm) with narrow size distributions, especially at the lowest ammonia concentrations. The average size, size distribution, morphology, and structure of particles were determined by dynamic light scattering (DLS), transmission electron microscopy (TEM), and UV/Visible absorption spectrophotometry. The influence of the saccharide structure (monosacharides versus disaccharides) on the size of silver particles is briefly discussed. The reduction of [Ag(NH(3))(2)](+) by maltose produced silver particles with a narrow size distribution with an average size of 25 nm, which showed high antimicrobial and bactericidal activity against Gram-positive and Gram-negative bacteria, including highly multiresistant strains such as methicillin-resistant Staphylococcus aureus. Antibacterial activity of silver nanoparticles was found to be dependent on the size of silver particles. A very low concentration of silver (as low as 1.69 mug/mL Ag) gave antibacterial performance.

Green synthesis, antimicrobial and cytotoxic effects of silver nanoparticles using Eucalyptus chapmaniana leaves extract

“…Additionally, the silver nanoparticles showed good inhibition activity towards C. albicans. The mechanism of inhibitory action of silver nanoparticles on microorganisms is not very well known [28,29] . However, several mechanisms have been proposed to explain the inhibitory effect of silver nanoparticles on bacteria.…”

Metal‐based nanoparticles in soil: Fate, behavior, and effects on soil invertebrates

“…Metal and metal oxide NPs have been introduced in many products with many different purposes. For example, they have been used in consumer products such as sunscreens and cosmetics (ZnO and TiO 2 ) [11,12], detergents and antibacterials (Ag) [13][14][15][16][17], paints (TiO 2 and Ag) [18,19], printer inks (Ag and Au) [20], and textiles (Ag) [13,21]. The applications of NPs also include electronics, medicine, chemistry, catalysis, and fuel additives [22].…”

Strain specificity in antimicrobial activity of silver and copper nanoparticles

“…Some of the silver nanoparticles also penetrate into the cells. It is reported that the bactericidal effect of silver nanoparticles decreases as the size increases and is also affected by the shape of the particles [23,24]. Although most studies have utilized spherical particles, truncated triangular shaped particles are reported to have greater bactericidal effect compared to that of spherical and rodshaped particles [24].…”