Similarity and differences in the physicochemical properties of lactate dehydrogenase isozymes from different tissues of Japanese sandfish Arctoscopus japonicus

Sugawara, Kotomi; Nakagawa, Mizuki; Yonezawa, Mika; Nakamura, Shuichi; Kidokoro, Shun‐ichi; Wakui, Hideki; Nunomura, Wataru

doi:10.1007/s12562-016-0972-1

Cited by 5 publications

(1 citation statement)

References 32 publications

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“…These genes may be potential target genes of miR-29b-1-5p. This finding indicates that the function and structure of LDH are highly conserved in skeletal muscle and testis, but LDHA encodes skeletal muscle-specific lactate dehydrogenase, which has unique characteristics in muscle tissue ( Sugawara et al., 2016 ). In addition, TCF12 is a MYOD coactivator involved in muscle cell differentiation ( Zhao and Hoffman, 2004 ).…”

Section: Discussionmentioning

confidence: 99%

MiR-29b-1-5p regulates the proliferation and differentiation of chicken primary myoblasts and analysis of its effective targets

Zhai

Yuan

et al. 2022

Poultry Science

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Several recent studies investigated the role of the miR-29 family in muscle development. However, only a few studies focused on chicken skeletal muscle. In the present study, cell cycle, 5-ethynyl-2’-deoxyuridine ( EdU ), cell counting kit-8 ( CCK-8 ), and other assays indicated that miR-29b-1-5p can inhibit the proliferation of chicken primary myoblasts ( CPMs ); the western blot assay and immunofluorescence detection of MYHC demonstrated that miR-29b-1-5p can promote the differentiation of myoblasts. The functional enrichment analysis revealed that the target genes of miR-29b-1-5p may be involved in muscle tissue development, muscle organ development, and striated muscle tissue development, which are biological processes related to muscle development. The correlation analysis showed that these 6 genes, that is, ankyrin repeat domain 9 ( ANKRD9 ), lactate dehydrogenase A ( LDHA ), transcription factor 12 ( TCF12 ), FAT atypical cadherin 1 ( FAT1 ), lin-9 homolog ( LIN9 ), and integrin beta 3 binding protein ( ITGB3BP ), can be used as effective candidate target genes of miR-29b-1-5p. Moreover, miR-29b-1-5p inhibits the expression of ANKRD9 by directly binding the 3′UTR of ANKRD9 . Overall, these data indicate that miR-29b-1-5p inhibits the proliferation of primary chicken myoblasts, stimulates their differentiation, and is involved in the process of muscle development and that its effective target gene is ANKRD9 . This study identified the molecular mechanism of miR-29b-1-5p in chicken muscle development.

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Section: Discussionmentioning

confidence: 99%