Simple and Efficient HPLC-Uv Quantitation of Etoposide and Its Cis-Isomer in Rat Micro-Volume Plasma and Tissue Samples: Application to Pharmacokinetic and Biodistribution Studies

Saadati, Roonak; Dadashzadeh, Simin

doi:10.1080/10826076.2011.585483

Cited by 13 publications

(6 citation statements)

References 25 publications

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“…It was found that the curve of etoposide shows the characteristic absorption peak at approximately 285 nm, similar to what has been reported in the literature. 39 From the spectra of ESCNs, it can be observed that an obvious absorption emerged at 285 nm, which revealed that the carriers of ESCNs degraded in the acidic condition after 24 hours, releasing etoposide out in the solution. This suggests that etoposide was likely packed in the dense scale-like spine structure of the SCNs.…”

Section: Resultsmentioning

confidence: 99%

pH-sensitive strontium carbonate nanoparticles as new anticancer vehicles for controlled etoposide release

Qian¹,

Sun²,

Zhu³

et al. 2012

IJN

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Strontium carbonate nanoparticles (SCNs), a novel biodegradable nanosystem for the pH-sensitive release of anticancer drugs, were developed via a facile mixed solvent method aimed at creating smart drug delivery in acidic conditions, particularly in tumor environments. Structural characterization of SCNs revealed that the engineered nanocarriers were uniform in size and presented a dumbbell-shaped morphology with a dense mass of a scale-like spine coating, which could serve as the storage structure for hydrophobic drugs. Chosen as a model anticancer agent, etoposide was effectively loaded into SCNs based on a simultaneous process that allowed for the formation of the nanocarriers and for drug storage to be accomplished in a single step. The etoposide-loaded SCNs (ESCNs) possess both a high loading capacity and efficient encapsulation. It was found that the cumulative release of etoposide from ESCNs is acid-dependent, and that the release rate is slow at a pH of 7.4; this rate increases significantly at low pH levels (5.8, 3.0). Meanwhile, it was also found that the blank SCNs were almost nontoxic to normal cells, and ESCN systems were evidently more potent in antitumor activity compared with free etoposide, as confirmed by a cytotoxicity test using an MTT assay and an apoptosis test with fluorescence-activated cell sorter (FACS) analysis. These findings suggest that SCNs hold tremendous promise in the areas of controlled drug delivery and targeted cancer therapy.

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Section: Resultsmentioning

confidence: 99%

pH-sensitive strontium carbonate nanoparticles as new anticancer vehicles for controlled etoposide release

Qian¹,

Sun²,

Zhu³

et al. 2012

IJN

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“…Other methods reported are for iv determination of ETD in dog plasma. 15 UV methods in rat plasma, [16][17] and iv administered mice. 18 Fluorescence method is more efficient when we compare with methods based on the UV detection.…”

Section: Introductionmentioning

confidence: 99%

“…And most importantly, this is so far the greenest and most environmentally benign bioanalytical method reported for ETD till date. [15][16][17][20][21][22] Yang and group 15 reported a gradient method, Pigatto et al 16 developed a method which involved use of Acetonitrile and non-green/lesser green. Method developed by Saadati et al 17 involved use of lesser green solvents like chloroform, Acetonitrile, and n-hexane.…”

Section: Introductionmentioning

confidence: 99%

Fluorescence Detection of Etoposide Encapsulated Mesoporous Silica Nanoparticles by Environmentally Benign Bioanalytical HPLC Method and its Application to Pharmacokinetic Study

Saroj¹,

Shah²,

Rajput³

2023

Ind. J. Pharm. Edu. Res.

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Introduction: Etoposide belongs to BCS Class IV suffering from solubility and permeability limitations. Thereby, hindering its bioavailability. Thereby, it is imperative to enhance its bioavailability by suitable formulation to achieve a desired therapeutic effect. Objectives: In present work, ETD was encapsulated into mesoporous silica nanoparticles (MSNs) with the aim of achieving bioavailability enhancement. Further, A simple, efficient, and environmentally benign HPLC method with highly sensitive fluorescence detection was developed for determination of Etoposide (ETD) in the mice plasma. Materials and Methods: The developed HPLC-FL method was sensitive enough to detect etoposide in the nano formulation in a plasma matrix with a high degree of accuracy and precision, taking Tapentadol as an internal standard. The chromatographic separation was conducted on a Waters symmetry C 18 column with a mobile phase composition of methanol: formate buffer (20mM) pH 3.9 in ratio 51:49 at a flow rate of 1 mL/min with excitation wavelength fixed at 247 nm and emission measured at 323 nm. Plasma sample pre-treatment was done following protein precipitation method. The developed bioanalytical method was validated successfully. Green metric assessment was done and Eco-indicators were employed which suggested the Eco-friendliness of developed method as well as supremacy over those available so far. Results: The bioavailability was enhanced 4.35 times as compared to ETO alone. The pharmacokinetic parameters of orally administered MSNs formulation were t 1/2 (Half-life) 12.12 hr, Peak plasma concentration C max 3.98, Area Under the Curve (AUC) 52.78 and Mean Residence Time 18.23 hr. Conclusion: It could be concluded that the developed mesoporous formulation played a major part in BA enhancement of ETD and the developed green method was highly efficient to serve the purpose of ETD determination from biological matrix.

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“…Literature research indicated various studies describing several analytical methods for the determination of ETP in many matrices including injectable dosage forms, biological fluids, and cancer cells. These methods are almost based on high-performance liquid chromatography [10][11][12][13][14][15][16][17][18][19], spectrofluorimetry [20], and micellar electrokinetic chromatography [21]. However, these methods are time-consuming, demand expensive instrumentation, need complicated sample preparation including extraction and preconcentration, and require an extensive use of organic solvents.…”

Section: Introductionmentioning

confidence: 99%

Selective Electrochemical Determination of Etoposide Using a Molecularly Imprinted Overoxidized Polypyrrole Coated Glassy Carbon Electrode

Hrichi

Monser

Adhoum

2019

International Journal of Electrochemistry

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A simple and efficient new electrochemical sensor based on molecularly imprinted polymer has been developed for selective detection of an anticancer agent Etoposide (ETP). The sensor was prepared by electropolymerization via cyclic voltammetry (CV) of pyrrole onto a glassy carbon electrode (GCE) in the presence of ETP molecules. The extraction of ETP molecules embedded in the polymeric matrix was carried out by overoxidation in sodium hydroxide medium using CV. Various important parameters affecting the performance of the imprinted film (MIP) coated sensor were studied and optimized using differential pulse voltammetry (DPV). Under optimal conditions, the sensor response exhibited a linear dependence on ETP concentration (R2= 0.999) over the range 5.0×10−7M – 1.0×10−5M with a LOD (3σ/m) of 2.8×10−9M. The precision (% RSD, n=6) of the proposed sensor for intra- and interdays was found to be 0.84 and 2.46%, respectively. The selectivity of MIP/GCE sensor toward ETP was investigated in the presence of different interfering molecules including excipients and ETP metabolites. The developed sensor showed great recognition ability toward ETP and was successfully applied for its determination in injectable dosage forms and biological human fluids.

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Simple and Efficient HPLC-Uv Quantitation of Etoposide and Its Cis-Isomer in Rat Micro-Volume Plasma and Tissue Samples: Application to Pharmacokinetic and Biodistribution Studies

Cited by 13 publications

References 25 publications

pH-sensitive strontium carbonate nanoparticles as new anticancer vehicles for controlled etoposide release

pH-sensitive strontium carbonate nanoparticles as new anticancer vehicles for controlled etoposide release

Fluorescence Detection of Etoposide Encapsulated Mesoporous Silica Nanoparticles by Environmentally Benign Bioanalytical HPLC Method and its Application to Pharmacokinetic Study

Selective Electrochemical Determination of Etoposide Using a Molecularly Imprinted Overoxidized Polypyrrole Coated Glassy Carbon Electrode

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