Simple and rapid field tests for brucellosis in livestock

Abstract

13 14Four simple and rapid field tests for the serodiagnosis of brucellosis in cattle, goat, 15 sheep and swine were developed. The performance of the assays was investigated using 16 serum samples collected in Portugal from animals originating from herds with a defined 17 sanitary status with respect to the presence of brucellosis. The sensitivity calculated for the 18 bovine, caprine, ovine and swine Brucella lateral flow assays based on results obtained for 19 samples collected from animals with culture confirmed brucellosis was 90%, 100%, 90% and 20 73%, respectively. None of the samples from animals from herds free of brucellosis reacted in 21 the flow assays indicating a high specificity. However, as expected, some degree of reactivity 22 was observed when testing selected serum samples that reacted non-specific in reference 23 tests for brucellosis. 24 25

Sensitivity and Specificity of Various Serological Tests for Detection of <i>Brucella spp</i>. Infection in Male Goats and Sheep

“…The (ICA) is easy and rapid to be done and it has several advantages that allow testing in the management of large numbers of serum samples without specialized training, expertise, electricity and nor expensive equipment and it used in the animals from developing countries and other migratory populations [19] [20]. Also these devices can storage without the need for cooling and the test results are obtained almost immediately and visual inspection with the naked eye [19].…”

“…Also these devices can storage without the need for cooling and the test results are obtained almost immediately and visual inspection with the naked eye [19]. Many scientific studies by Smits et al [21]; Dey et al [22] refer to that ICA is accurate as compared with the standard microscopic agglutination test (MAT) also the sensitivity and specificity of LAT are 88% and 98%, respectively [23].…”

Sero-prevalence of camel brucellosis in three abbatoirs of Nothern Nigeria

“…The LFA has several practical advantages which include the fact that the use of the LFA does not require specific training, expertise, electricity or expensive equipment, and the test devices may be stored without the need for refrigeration. The test results are obtained almost instantaneously and by visual inspection with the unaided eye (Abdoel et al, 2008). The test has been compared to Competitive ELISA (cELISA), and was reported to be even more sensitive and specific than cELISA (Bronsvoort et al, 2009).…”

“…Samples positive by RBPT were further tested by Brucella LFiA done as described by Abdoel et al (2008) …”

“…Lateral flow immunoassay was performed as described by Abdoel et al (2008) using bovine Brucella abortus kit (Royal Tropical Institute, the Netherlands). The flow kit was removed from the packaging and placed on a bench top with the test window facing upwards.…”