Simple method of Arabidopsis thaliana cultivation in liquid nutrient medium

Siedlecka, A.; Krupa, Zbigniew

doi:10.1007/s11738-002-0007-7

Cited by 13 publications

(9 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Uniform, 6-week-old seedlings were transplanted to (i) pots (Ø 10 cm) containing the same substrate or (ii) hydroponics culture filled with 0.3 dm 3 of a Hoagland solution (Arnon and Hoagland, 1940) modified by Siedlecka and Krupa (2002). The nutrient solution was continuously aerated and renewed weekly.…”

Section: Methodsmentioning

confidence: 99%

Biological mode of action of a nitrophenolates-based biostimulant: case study

2014

View full text Add to dashboard Cite

The challenges facing modern plant production involve (i) responding to the demand for food and resources of plant origin from the world's rapidly growing population, (ii) coping with the negative impact of stressful conditions mainly due to anthropopressure, and (iii) meeting consumers' new requirements and preferences for food that is high in nutritive value, natural, and free from harmful chemical additives. Despite employing the most modern plant cultivation technologies and the progress that has been made in breeding programs, the genetically-determined crop potential is still far from being fully exploited. Consequently yield and quality are often reduced, making production less, both profitable and attractive. There is an increasing desire to reduce the chemical input in agriculture and there has been a change toward integrated plant management and sustainable, environmentally-friendly systems. Biostimulants are a category of relatively new products of diverse formulations that positively affect a plant's vital processes and whose impact is usually more evident under stressful conditions. In this paper, information is provided on the mode of action of a nitrophenolates-based biostimulant, Atonik, in model species and economically important crops grown under both field and controlled conditions in a growth chamber. The effects of Atonik on plant morphology, physiology, biochemistry (crops and model plant) and yield and yield parameters (crops) is demonstrated. Effects of other biostimulants on studied in this work processes/parameters are also presented in discussion.

show abstract

Section: Methodsmentioning

confidence: 99%

Biological mode of action of a nitrophenolates-based biostimulant: case study

2014

View full text Add to dashboard Cite

show abstract

“…One strategy to circumvent the problem of the low yield of leaf tissue from Arabidopsis would be to develop a new cultivation system based upon hydroponic growth. Several hydroponic culture systems have been reported for Arabidopsis (Rodecap et al 1994, Nanjo et al 1999, Toda et al 1999, Siedlecka and Krupa 2002), some of which use rock wool or sponges as the growth base for the seedlings (Hirai et al 1995, Gibeaut et al 1997, Arteca and Arteca 2000) and most have been developed as small experimental units. The need for a culture system that combined high biomass production for advanced biochemical and biophysical studies with controlled cultivation (of wild‐type and selected mutant plants) prompted us to develop a new cultivation system for Arabidopsis .…”

Section: Introductionmentioning

confidence: 99%

A convenient and versatile hydroponic cultivation system for Arabidopsis thaliana

Norén

Svensson

Andersson

2004

Physiologia Plantarum

View full text Add to dashboard Cite

A versatile two-step cultivation procedure for Arabidopsis thaliana is described for the production of large quantities of leaf material suitable for biochemical and biophysical analysis. The first step comprises a miniature greenhouse made out of a plastic pipette box to grow the seedlings to the six-leaf stage. For continued growth, the seedlings are transferred to hydroponic cultivation using an opaque container covered by a styrofoam lid. Transfer of the small seedlings to hydroponic culture is facilitated by growth in separate pipette tips, which protects vulnerable roots from damage. The hydroponic cultivation system is easy to scale-up and produces large amounts of relatively large leaves and roots. This hydroponic system produces enough plant material to make Arabidopsis a feasible model for biochemical and biophysical experiments, which can be combined with the available genetic information to address various aspects of plant functional genomics.

show abstract

“…3 Cadmium interacts with the water balance 4 and damages the photosynthetic apparatus (photosystems II and I). 5 Cadmium inhibits oxidative mitochondrial phosphorylation, 6 reduces activity of plasma membrane ATPase 7 and strongly affects the activity of several enzymes, such as glucose-6-phosphate dehydrogenase, malic enzyme, isocitrate dehydrogenase, 8 Rubisco and carbonic anhydrase. 9 Cadmium ions can inhibit the activity of several antioxidative enzymes.…”

mentioning

confidence: 99%

The effect of soil properties on cadmium bonds to organic substances of spinach biomass

Pavlı́ková

Pavlı́k

Vašíčková

et al. 2002

Applied Organom Chemis

View full text Add to dashboard Cite

The main objective of this study was focused on sequential fractionation of spinach biomass, partial characterization of cadmium-binding compounds and determination of the cadmium concentration in these fractions. Spinach was cultivated after application of sewage sludge on two soils of different properties and total cadmium content in a model pot experiment. Two non-polar solvents (light petroleum and ethyl acetate), a polar solvent (methanol) and two solvent mixtures (methanol H 2 O & H 2 O and methanol H 2 O HCl) were used in the extraction series. Isolated fractions were characterized by IR spectroscopy. The lower weight of light petroleum (aliphatic hydrocarbons), ethyl acetate (fatty acids), and methanol (carboxylic acids, mainly acids of citric cycle, pyrrole substances) fractions in sludge treatments compared with the controls showed that the metabolism of plant natural products was inhibited by toxins from sludge. The weight of methanol fraction was dominant for all treatments. The cadmium determined in these fractions was low. The majority of cadmium was found in the methanol H 2 O & H 2 O (oligopeptides, mainly phytochelatins, extractable polypeptides, proteins) and methanol H 2 O HCl (compounds isolated from cell walls and cytoskeleton after hydrolysis) fractions. The effect of toxin stress only appears to be on the quantity of substances, as there is no qualitative change observed in the isolated fractions (measured values of IR spectra bands did not differ for treatments tested). The application of sequential analysis for spinach biomass extraction makes it possible to investigate the complex effects of cadmium and other toxins on plant metabolism.

show abstract

Simple method of Arabidopsis thaliana cultivation in liquid nutrient medium

Cited by 13 publications

References 5 publications

Biological mode of action of a nitrophenolates-based biostimulant: case study

Biological mode of action of a nitrophenolates-based biostimulant: case study

A convenient and versatile hydroponic cultivation system for Arabidopsis thaliana

The effect of soil properties on cadmium bonds to organic substances of spinach biomass

Contact Info

Product

Resources

About