Simple micropatterning of biomolecules on a diazoketo-functionalized photoresist

Ganesan, Ramakrishnan; Yoo, So Young; Choi, Jae‐Hak; Lee, Sang Yup; Kim, Jin-Baek

doi:10.1039/b709774c

Cited by 24 publications

(17 citation statements)

References 44 publications

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“…[125][126][127][128][129] These materials have been proposed as biocompatible photoresists for patterning cells, DNA, or proteins by subsequent functionalization with appropriate ligands.…”

Section: Photosensitive Polymer Brushesmentioning

confidence: 99%

Light‐Triggered Multifunctionality at Surfaces Mediated by Photolabile Protecting Groups

Cui

Miguel

Campo

2012

Macromol. Rapid Commun.

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Photoremovable protecting groups (PRPGs) are applied to organic surfaces, thin polymer films, and hydrogels to achieve light-based remote control of their (bio)chemical and physical properties. These can be localized (i.e. patterned), tunable by exposure dose, and generated on-demand. Using PRPGs with independent response to different wavelengths, multifunctional materials with a number of individually addressable functional states can be generated. Light-triggered polymerization, crosslinking, and degradation processes as well as release of attached molecules can be realized. Light-responsive surfaces and materials based on PRPGs open interesting possibilities for the next generation of instructive materials for cell culture and tissue regeneration.

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“…[125][126][127][128][129] These materials have been proposed as biocompatible photoresists for patterning cells, DNA, or proteins by subsequent functionalization with appropriate ligands.…”

Section: Photosensitive Polymer Brushesmentioning

confidence: 99%

Light‐Triggered Multifunctionality at Surfaces Mediated by Photolabile Protecting Groups

Cui

Miguel

Campo

2012

Macromol. Rapid Commun.

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“…Biomolecules such as DNA and protein were immobilized on the PAA‐grafted PTFE films by a similar method published in our previous articles 31, 32. To immobilize the p‐DNA onto the PAA‐grafted regions, a solution containing 15 mM NHS, 45 mM EDC and 50 μg/mL of the p‐DNA was applied over the PAA‐grafted PTFE films and allowed to react overnight.…”

Section: Methodsmentioning

confidence: 99%

Patterned immobilization of biomolecules by using ion irradiation‐induced graft polymerization

Choi

Ganesan

Kim

et al. 2009

J. Polym. Sci. A Polym. Chem.

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A new method for biomolecular patterning based on ion irradiation‐induced graft polymerization was demonstrated in this study. Ion irradiation on a polymer surface resulted in the formation of active species, which was further used for surface‐initiated graft polymerization of acrylic acid. The results of the grafting study revealed that the surface graft polymerization using 20 vol % of acrylic acid on the poly(tetrafluoroethylene) (PTFE) film irradiated at the fluence of 1 × 1015 ions/cm2 for 12 h was the optimum graft polymerization condition to achieve the maximum grafting degree. The results of the fluorescence microscopy also revealed that the optimum fluence to achieve the maximum fluorescence intensity was 1 × 1015 ions/cm2. The grafting of acrylic acid on the PTFE surfaces was confirmed by a fluorescence labeling method. The grafted PTFE films were used for the immobilization of amine‐functionalized p‐DNA, followed by hybridization with fluorescently tagged c‐DNA. Biotin‐amine was also immobilized on the acrylic acid grafted PTFE surfaces. Successful biotin‐specific binding of streptavidin further confirmed the potential of this strategy for patterning of various biomolecules. © 2009 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 47: 6124–6134, 2009

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“…83 Due to the possibility to perform the ketene generation without introducing any other reactants in the reaction mixture, this has been used to perform photolithographic attachments of functional organic molecules via ester or amide linkages 84 or to pattern cells and biomolecules by diazoketo-functionalized photoresists after entrapment of the ketene with water. 85 Ketenes derived from the Wolff rearrangement can be intramolecularly trapped by the presence of a hydroxyl group. Depending on the type of reactions conditions used to initiate the nitrogen extrusion reaction of a-diazo-b-ketoesters 66, furanones 67 or lactones 68 can be obtained (Scheme 19).…”

Section: Reactions Of Ketenes Derived From the Wolff Rearrangement Wimentioning

confidence: 99%

3.19 The Wolff Rearrangement

Candeias

Trindade

Góis

et al. 2014

Comprehensive Organic Synthesis II

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Simple micropatterning of biomolecules on a diazoketo-functionalized photoresist

Cited by 24 publications

References 44 publications

Light‐Triggered Multifunctionality at Surfaces Mediated by Photolabile Protecting Groups

Light‐Triggered Multifunctionality at Surfaces Mediated by Photolabile Protecting Groups

Patterned immobilization of biomolecules by using ion irradiation‐induced graft polymerization

3.19 The Wolff Rearrangement

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