Simultaneous analysis of a series of phosphorylated sugars in small tissue samples by anion exchange chromatography and pulsed amperometric detection

Bruijn, S.M. de; Visser, Richard G. F.; Vreugdenhil, D.

doi:10.1002/(sici)1099-1565(199905/06)10:3<107::aid-pca449>3.0.co;2-l

Cited by 15 publications

(12 citation statements)

References 12 publications

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“…Seeds were extracted for carbohydrates and enzymes as previously described (Blöchl et al, 2005). Enzyme activities were assayed according to De Bruijn et al (1999) and Goulard et al (2001) in controls, DGJ and DGJ/galactose-treated seeds only.…”

Section: Methodsmentioning

confidence: 99%

Inhibition of raffinose oligosaccharide breakdown delays germination of pea seeds

Blöchl

Peterbauer

Richter

2007

Journal of Plant Physiology

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Section: Methodsmentioning

confidence: 99%

Inhibition of raffinose oligosaccharide breakdown delays germination of pea seeds

Blöchl

Peterbauer

Richter

2007

Journal of Plant Physiology

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“…Consequently, less-polar compounds have a strong interaction with the apolar stationary phase, and are more retained on the column than polar analytes (e.g., most primary metabolites such as carbohydrates, glycolytic intermediates, and sugar phosphates) (Dunn and Ellis, 2005). High performance anion-exchange liquid chromatography (HPAEC) with pulsed amperometric detection (PAD) has been widely used in the past for the analysis of such highly polar metabolites, including underivatized carbohydrate-related compounds and other intermediary metabolites from animal tissues (Swezey, 1995), Escherichia coli cell extracts (Bhattacharya et al, 2005), potato (de Bruijn, Visser, & Vreugdenhil, 1999), and also from A. thaliana tissues (Sekiguchi et al, 2004). Nonetheless, the mobile phases used with commercial HPAEC systems (Dionex) contain high concentrations of sodium hydroxide and sodium acetate, and, therefore, are not MScompatible.…”

Section: B Liquid Chromatography-mass Spectrometry (Lc-ms)mentioning

confidence: 99%

Mass spectrometry‐based plant metabolomics: Metabolite responses to abiotic stress

Jorge

Rodrigues

Caldana

et al. 2015

Mass Spectrometry Reviews

298

210

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Metabolomics is one omics approach that can be used to acquire comprehensive information on the composition of a metabolite pool to provide a functional screen of the cellular state. Studies of the plant metabolome include analysis of a wide range of chemical species with diverse physical properties, from ionic inorganic compounds to biochemically derived hydrophilic carbohydrates, organic and amino acids, and a range of hydrophobic lipid-related compounds. This complexitiy brings huge challenges to the analytical technologies employed in current plant metabolomics programs, and powerful analytical tools are required for the separation and characterization of this extremely high compound diversity present in biological sample matrices. The use of mass spectrometry (MS)-based analytical platforms to profile stress-responsive metabolites that allow some plants to adapt to adverse environmental conditions is fundamental in current plant biotechnology research programs for the understanding and development of stress-tolerant plants. In this review, we describe recent applications of metabolomics and emphasize its increasing application to study plant responses to environmental (stress-) factors, including drought, salt, low oxygen caused by waterlogging or flooding of the soil, temperature, light and oxidative stress (or a combination of them). Advances in understanding the global changes occurring in plant metabolism under specific abiotic stress conditions are fundamental to enhance plant fitness and increase stress tolerance. © 2015 Wiley Periodicals, Inc. Mass Spec Rev 35:620-649, 2016.

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“…Estimation of glucose 6-phosphate by enzymatic method Extraction of phosphorylated sugars was performed as described earlier (de Bruijn et al 1999). Detection and quantiWcation of glucose 6-phosphate was performed essentially as described (Carpita and Kanabus 1987) except neither ATP nor hexokinase was added to the reactions.…”

Section: Quantitative Estimation Of Sugarsmentioning

confidence: 99%

Phosphate starvation responses are mediated by sugar signaling in Arabidopsis

Karthikeyan

Varadarajan

Jain

et al. 2006

Planta

212

168

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Phosphate (Pi) is one of the least available plant nutrients in soils. It is associated with dynamic changes in carbon fluxes and several crucial processes that regulate plant growth and development. Pi levels regulate the expression of large number of genes including those involved in photosynthesis and carbon metabolism. Herein we show that sugar is required for Pi starvation responses including changes in root architecture and expression of phosphate starvation induced (PSI) genes in Arabidopsis. Active photosynthesis or the supplementation of sugar in the medium was essential for the expression of PSI genes under Pi limiting conditions. Expression of these genes was not only induced by sucrose but also detected, albeit at reduced levels, with other metabolizable sugars. Non-metabolizable sugar analogs did not induce the expression of PSI genes. Although sugar input appears to be down-stream of initial Pi sensing, it is absolutely required for the completion of the PSI signaling pathway. Altered expression of PSI genes in the hexokinase signaling mutant gin2 indicates that hexokinase-dependent signaling is involved in this process. The study provides evidence for requirement of sugars in PSI signaling and evokes a role for hexokinase in some components of Pi response mechanism.

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Simultaneous analysis of a series of phosphorylated sugars in small tissue samples by anion exchange chromatography and pulsed amperometric detection

Cited by 15 publications

References 12 publications

Inhibition of raffinose oligosaccharide breakdown delays germination of pea seeds

Inhibition of raffinose oligosaccharide breakdown delays germination of pea seeds

Mass spectrometry‐based plant metabolomics: Metabolite responses to abiotic stress

Phosphate starvation responses are mediated by sugar signaling in Arabidopsis

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