Simultaneous analysis of spectinomycin, halquinol, zilpaterol, and melamine in feedingstuffs by ion-pair liquid chromatography–tandem mass spectrometry

Molognoni, Luciano; Souza, Naielly Coelho de; Ploêncio, Leandro Antunes de Sá; Micke, Gustavo Amadeu; Daguer, Heitor

doi:10.1016/j.chroma.2018.07.048

Cited by 16 publications

(11 citation statements)

References 27 publications

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“…Antibiotics are bioactive substances used against bacteria as a therapeutic, metaphylaxis or prophylactic agent both in humans and animals [200,201,202]. In livestock, some antibiotics are included in animal diets as growth promoter (e.g., monensin, narasin, ractopamine), decrease feed conversion, improve feed efficiency, and overall cost-effectiveness of animal production systems [203,204].…”

Section: Determinations Designed For Feed and Feed Ingredientsmentioning

confidence: 99%

“…Reverse-phase chromatography requires a relatively inexpensive column that is usually available in laboratories and which analytical instrumentation providers generally keep in stock. Additionally, a more effective separation was archived using heptafluorobutyric acid in the mobile phase [202].…”

Section: Determinations Designed For Feed and Feed Ingredientsmentioning

confidence: 99%

“…It is here that mass spectrometry has been useful, by reducing costs and response time. [185,202,209].…”

Section: Determinations Designed For Feed and Feed Ingredientsmentioning

confidence: 99%

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Liquid Chromatography Analysis of Common Nutritional Components, in Feed and Food

Cortés-Herrera

Artavia

Leiva

et al. 2018

Foods

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Food and feed laboratories share several similarities when facing the implementation of liquid-chromatographic analysis. Using the experience acquired over the years, through application chemistry in food and feed research, selected analytes of relevance for both areas were discussed. This review focused on the common obstacles and peculiarities that each analyte offers (during the sample treatment or the chromatographic separation) throughout the implementation of said methods. A brief description of the techniques which we considered to be more pertinent, commonly used to assay such analytes is provided, including approaches using commonly available detectors (especially in starter labs) as well as mass detection. This manuscript consists of three sections: feed analysis (as the start of the food chain); food destined for human consumption determinations (the end of the food chain); and finally, assays shared by either matrices or laboratories. Analytes discussed consist of both those considered undesirable substances, contaminants, additives, and those related to nutritional quality. Our review is comprised of the examination of polyphenols, capsaicinoids, theobromine and caffeine, cholesterol, mycotoxins, antibiotics, amino acids, triphenylmethane dyes, nitrates/nitrites, ethanol soluble carbohydrates/sugars, organic acids, carotenoids, hydro and liposoluble vitamins. All analytes are currently assayed in our laboratories.

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Section: Determinations Designed For Feed and Feed Ingredientsmentioning

confidence: 99%

Section: Determinations Designed For Feed and Feed Ingredientsmentioning

confidence: 99%

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Liquid Chromatography Analysis of Common Nutritional Components, in Feed and Food

Cortés-Herrera

Artavia

Leiva

et al. 2018

Foods

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“…To date, many methods have been used to measure spectinomycin and lincomycin in animal-derived foods and animal feedstuffs, including fluorescent latex immunoassay (FLI) [11], micellar electrokinetic capillary chromatography combined with ultraviolet detection (MEKC-UVD) [12], enzyme-linked immunosorbent assay (ELISA) [13], high-performance liquid chromatography with electrochemical detection (HPLC-ECD) [14,15], HPLC with fluorescence detection (FLD) [16], HPLC-UVD [17,18], HPLC with evaporative light-scattering detection (ELSD) [19,20], hydrophilic interaction chromatography with mass spectrometry (HILIC-MS) [21], HILIC tandem MS (MS/MS) [22], HPLC-MS [23], HPLC-MS/MS [24][25][26][27][28], gas chromatography-nitrogen phosphorus detection (GC-NPD) [29,30] and GC-MS [30]. The FLI, ELISA, ECD, FLD, UVD and ELSD methods have low sensitivity, specificity, recovery and precision and have many limitations.…”

Section: Introductionmentioning

confidence: 99%

“…The FLI, ELISA, ECD, FLD, UVD and ELSD methods have low sensitivity, specificity, recovery and precision and have many limitations. Molognoni et al [22] developed a liquid-liquid extraction (LLE) method combined with HILIC-MS/MS for the determination of spectinomycin, halquinol, zilpaterol and melamine residues in animal feedstuffs with good recovery and precision. Juan et al [27] reported an accelerated solvent extraction (ASE) method for the trace analysis of macrolide and lincosamide antibiotics in meat and milk using HPLC-MS/MS, and the method was fast, sensitive and automatic, making it suitable for the determination of macrolide and lincosamide residues in meat and milk.…”

Section: Introductionmentioning

confidence: 99%

Quantitative Analysis of Spectinomycin and Lincomycin in Poultry Eggs by Accelerated Solvent Extraction Coupled with Gas Chromatography Tandem Mass Spectrometry

Wang

Xie

et al. 2020

Foods

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A method based on accelerated solvent extraction (ASE) coupled with gas chromatography tandem mass spectrometry (GC-MS/MS) was developed for the quantitative analysis of spectinomycin and lincomycin in poultry egg (whole egg, albumen and yolk) samples. In this work, the samples were extracted and purified using an ASE350 instrument and solid-phase extraction (SPE) cartridges, and the parameters of the ASE method were experimentally optimized. The appropriate SPE cartridges were selected, and the conditions for the derivatization reaction were optimized. After derivatization, the poultry egg (whole egg, albumen and yolk) samples were analyzed by GC-MS/MS. This study used blank poultry egg (whole egg, albumen and yolk) samples to evaluate the specificity, sensitivity, linearity, recovery and precision of the method. The linearity (5.6–2000 μg/kg for spectinomycin and 5.9–200 μg/kg for lincomycin), correlation coefficient (≥0.9991), recovery (80.0%–95.7%), precision (relative standard deviations, 1.0%–3.4%), limit of detection (2.3–4.3 μg/kg) and limit of quantification (5.6–9.5 μg/kg) of the method met the requirements for EU parameter verification. Compared with traditional liquid–liquid extraction methods, the proposed method is fast and consumes less reagents, and 24 samples can be processed at a time. Finally, the feasibility of the method was evaluated by testing real samples, and spectinomycin and lincomycin residues in poultry eggs were successfully detected.

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Determination of Polar Veterinary Drugs and Melamine in Feeds: Method Using Ion-Pair Reversed-Phase or Hydrophilic Interaction Liquid Chromatography Coupled to Tandem Mass Spectrometry

Molognoni,

de Melo,

de Oliveira

et al. 2024

Methods and Protocols in Food Science

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Simultaneous analysis of spectinomycin, halquinol, zilpaterol, and melamine in feedingstuffs by ion-pair liquid chromatography–tandem mass spectrometry

Cited by 16 publications

References 27 publications

Liquid Chromatography Analysis of Common Nutritional Components, in Feed and Food

Liquid Chromatography Analysis of Common Nutritional Components, in Feed and Food

Quantitative Analysis of Spectinomycin and Lincomycin in Poultry Eggs by Accelerated Solvent Extraction Coupled with Gas Chromatography Tandem Mass Spectrometry

Determination of Polar Veterinary Drugs and Melamine in Feeds: Method Using Ion-Pair Reversed-Phase or Hydrophilic Interaction Liquid Chromatography Coupled to Tandem Mass Spectrometry

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