Simultaneous changes in lipid composition, fluidity and enzyme activity in piglet intestinal brush border membrane as affected by dietary polyunsaturated fatty acid deficiency

Daveloose, Denis; Linard, Alain; Arfi, T S; Viret, Jacques; Christon, Raymond

doi:10.1016/0005-2760(93)90102-f

Cited by 36 publications

(27 citation statements)

References 44 publications

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“…However, in our case, this inverse relationship did not exist, since an increase in the cholesterol/ phospholipid ratio in brush-border membranes prepared from SHR was accompanied by an increase in the membrane fluidity. The analysis of fatty acids in WKY rats revealed the presence of four major fatty acids, palmitic (16:0), stearic (18:0), oleic (18:1 n-9) and linoleic (18:2 n-6), as previously reported for rats (35) and other anomals (36). Hypertension did not produce large differences in the fatty acid composition of jejunal membranes, since it was similar for SHR.…”

Section: Discussionsupporting

confidence: 49%

Lipid composition and fluidity in the jejunal brush-border membrane of spontaneously hypertensive rats. Effects on activities of membrane-bound proteins

1996

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onThe lipid composition and fluidity of jejunal brush-border membrane vesicles (BBMV) have been studied in spontaneously hypertensive rats (SHR) and normotensive Wistar Kyoto (WKY) rats. The activities of both Na+-dependent D-glucose cotransport and Na+-H * antiport have also been determined. A significant increase in the level of free cholesterol was observed in jejunal BBMV from SHR compared to WKY rats. Since phospholipid values did not change in either group of animals, a significant enhancement in the free cholesterol/phospholipid ratio was observed in SHR. A decrease in the levels of phosphatidylethanolamine together with an increase in the values of phosphatidylserine was observed in hypertensive rats. Although the content of phosphatidylcholine (PC) and sphingomyelin (SM) was not singificantly altered in SHR, the ratio PC/SM significantly increased in these animals when compared to WKY rats. The major fatty acids present in bursh-border membranes prepared from SHR and WKY rats were palmitic (16:0), stearic (18:0), oleic (18:1, n-9) and linoleic (18:2, n-6), and the fatty acid composition was not modified by the hypertension. A decreased fluorescence polarization, i.e., increased membrane fluidity, was observed in SHR, which was not correlated to the increased ratio of cholesterol/phospholipid found in the brush-border membrane isolated from these animals. These structural changes found in SHR were associated to an enhancement in both Na § D-glucose transport and Na~--H -~ antiport activity in the jejunal BBMV of SHR.

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Section: Discussionsupporting

confidence: 49%

Lipid composition and fluidity in the jejunal brush-border membrane of spontaneously hypertensive rats. Effects on activities of membrane-bound proteins

1996

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“…It is therefore premature to determine whether n-3 or n-6 PUFA are most beneficial, or to generalize to all animals. However, it is well established that membrane fluidity and permeability are significantly increased by all high PUFA diets (Ernst, 1994) and decreased by diets poor in PUFA (Daveloose et al, 1993). In this study, we show that semipalmated sandpipers modify their muscle membranes by feeding on Corophium as they prepare for long-distance flight.…”

Section: Flight Muscle Membranes Are Modifiedmentioning

confidence: 49%

“…Multiple lines of evidence show that muscle performance can be affected by the n-3 PUFA content of membrane phospholipids (PL) via changes in fluidity and permeability (Daveloose et al, 1993;Ernst, 1994;Stillwell and Wassal, 2003). For example, unusually high levels of n-3 PUFA have been found in the membrane PL of highly aerobic muscles such as hummingbird flight muscles (Infante et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Performance-enhancing role of dietary fatty acids in a long-distance migrant shorebird: the semipalmated sandpiper

Maillet

Weber

2006

Journal of Experimental Biology

114

131

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dietary n-3 PUFA are incorporated in tissue lipids in less than 2 weeks. During the stopover, the double bond index of muscle PL increases by 25% and the fatty acid profiles of both muscle PL and adipose NL converge with that of the diet. However, >50% of dietary n-3 PUFA are converted to other fatty acids before storage, mainly to oleate (18:1), possibly because monounsaturates offer a compromise between high energy density and ease of mobilization. This study shows that long-distance migrant birds can (1) use natural diets rich in specific lipids to prime flight muscles for endurance exercise, and (2) modify dietary fatty acids before storing them as fuel.

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“…This diet is intriguing because changes in the n-3 PUFA content of phospholipids affect membrane fluidity (Daveloose et al, 1993;Ernst, 1994;Stillwell and Wassal, 2003), thereby modifying muscle performance. We have recently shown that semipalmated sandpipers refueling on Corophium greatly increase the n-3 PUFA content of their tissues (Maillet and Weber, 2006).…”

Section: Introductionmentioning

confidence: 99%

Relationship between n-3 PUFA content and energy metabolism in the flight muscles of a migrating shorebird: evidence for natural doping

Maillet

Weber

2007

Journal of Experimental Biology

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SUMMARY During their fall migration from the Arctic to South America, semipalmated sandpipers Calidris pusilla stop in the Bay of Fundy (east coast of Canada) before flying non-stop for ∼4500 km across the ocean. Refueling birds double their body mass by feeding on Corophium volutator, an amphipod containing high amounts of n-3 polyunsaturated fatty acids (n-3 PUFA), particularly eicosapentaenoic (20:5) and docosahexaenoic acid (22:6). In mammals, high dietary intake of n-3 PUFA is known to increase capacity for oxidative metabolism. Therefore, we hypothesized that tissue incorporation of n-3 PUFA would be associated with increases in the activity of key muscle enzymes to upregulate energy metabolism for prolonged exercise. Birds were collected at various stages of fat loading to monitor changes in lipid composition and flight muscle enzymes simultaneously. Enzymes were measured to assess oxidative capacity [citrate synthase (CS)],β-oxidation [carnitine palmitoyl transferase (CPT) and 3-hydroxyacyl dehydrogenase (HOAD)] and glycolytic capacity [lactate dehydrogenase (LDH)]. Changes in the fatty acid composition of muscle membranes (phospholipids) and fuel reserves (neutral lipids) were measured separately to distinguish between membrane-related and systemic effects of n-3 PUFA. Results show that muscle CS and HOAD are stimulated during refueling and that their activities are correlated with n-3 PUFA content in phospholipids (22:6 for CS, 20:5 for HOAD)and in neutral lipids (20:5 for CS). This suggests that 20:5 and 22:6 have different effects on energy metabolism and that they act via changes in membrane structure and systemic mechanisms. CPT and LDH did not change during refueling, but LDH activity was significantly related to the n-3 PUFA content of fuel reserves. This study shows that oxidative capacity increases rapidly during refueling and supports the idea that dietary n-3 PUFA are used as molecular signals to prime flight muscles of some long-distance migrants for extreme exercise.

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Simultaneous changes in lipid composition, fluidity and enzyme activity in piglet intestinal brush border membrane as affected by dietary polyunsaturated fatty acid deficiency

Cited by 36 publications

References 44 publications

Lipid composition and fluidity in the jejunal brush-border membrane of spontaneously hypertensive rats. Effects on activities of membrane-bound proteins

Lipid composition and fluidity in the jejunal brush-border membrane of spontaneously hypertensive rats. Effects on activities of membrane-bound proteins

Performance-enhancing role of dietary fatty acids in a long-distance migrant shorebird: the semipalmated sandpiper

Relationship between n-3 PUFA content and energy metabolism in the flight muscles of a migrating shorebird: evidence for natural doping

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