Simultaneous detection of lactate and glucose by integrated printed circuit board based array sensing chip

Li, Xuelian; Zang, Jianfeng; Liu, Yingshuai; Lu, Zhisong; Li, Qing; Li, Chang Ming

doi:10.1016/j.aca.2013.02.011

Cited by 24 publications

(14 citation statements)

References 26 publications

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“…Hence, in this paper we present the electrochemical and surface characteristics of the sensing electrodes as received by the manufacturer and also after our surface treatment process. Glucose oxidase is subsequently covalently immobilized on the chemically pre-treated, Au plated working electrodes, as opposed to previously presented PCB-implemented glucose sensors [10][11][12][13], resulting in a lower limit of detection of 500 µM.…”

Section: Introductionmentioning

confidence: 99%

Commercially Fabricated Printed Circuit Board Sensing Electrodes for Biomarker Electrochemical Detection: The Importance of Electrode Surface Characteristics in Sensor Performance

2018

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Here we report the first PCB-implemented electrochemical glucose biosensor usingcovalently immobilized glucose oxidase (GOx) on the commercially fabricated PCB electrodesurface, taking particular care on the electrode surface characteristics and their effect on sensorperformance. Based on the results, this assay exhibits a highly linear response from 500 μM to 20mM (R = 0.9961) and a lower limit of detection of 500 μM.

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Section: Introductionmentioning

confidence: 99%

Commercially Fabricated Printed Circuit Board Sensing Electrodes for Biomarker Electrochemical Detection: The Importance of Electrode Surface Characteristics in Sensor Performance

2018

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“…Table 1 compares sensor performance parameters for biosensors calibrated in PBS to other enzyme-based biosensors in literature. 5,23,58–61 The upper limit of the linear range of lactate sensors on glass slides is low compared with similar lactate sensors on flexible laminate. Variation in enzyme functionalization may have been the cause.…”

Section: Resultsmentioning

confidence: 96%

“…9 Several groups have used enzyme-based multianalyte biosensors for measurements in vivo, 10–13 on-line, 14 off-line, 15–17 and in vitro. 18–32 However, their use with organ-on-a-chip platform 5,33 or in cell cultures 34 is not widespread. Bavli et al 5 and Misun et al 33 have integrated glucose and lactate sensors into custom microfluidic systems for organ-on-a-chip.…”

Section: Introductionmentioning

confidence: 99%

Simple Fabrication of Flexible Biosensor Arrays Using Direct Writing for Multianalyte Measurement from Human Astrocytes

Nolan

DeLong

et al. 2020

SLAS Technology

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Simultaneous measurements of glucose, lactate, and neurotransmitters (e.g., glutamate) in cell culture over hours and days can provide a more dynamic and longitudinal perspective on ways neural cells respond to various drugs and environmental cues. Compared with conventional microfabrication techniques, direct writing of conductive ink is cheaper, faster, and customizable, which allows rapid iteration for different applications. Using a simple direct writing technique, we printed biosensor arrays onto cell culture dishes, flexible laminate, and glass to enable multianalyte monitoring. The ink was a composite of PEDOT:PSS conductive polymer, silicone, activated carbon, and Pt microparticles. We applied 0.5% Nafion to the biosensors for selectivity and functionalized them with oxidase enzymes. We characterized biosensors in phosphate-buffered saline and in cell culture medium supplemented with fetal bovine serum. The biosensor arrays measured glucose, lactate, and glutamate simultaneously and continued to function after incubation in cell culture at 37 °C for up to 2 days. We cultured primary human astrocytes on top of the biosensor arrays and placed arrays into astrocyte cultures. The biosensors simultaneously measured glucose, glutamate, and lactate from astrocyte cultures. Direct writing can be integrated with microfluidic organ-on-a-chip platforms or as part of a smart culture dish system. Because we print extrudable and flexible components, sensing elements can be printed on any 3D or flexible substrate.

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“…In the work of Li et al, 141 an integrated PCB based array sensing chip with a high signal to noise ratio and a high sensitivity was developed for simultaneous detection of lactate and glucose in serum. In this case, lactate oxidase and glucose oxidase were immobilized on gold plated electrodes, and the amperometric response was recorded for increasing lactate/glucose concentrations.…”

Section: Technology Overview and Demonstrated Prototypesmentioning

confidence: 99%

The lab-on-PCB approach: tackling the μTAS commercial upscaling bottleneck

2017

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Commercialization of lab-on-a-chip devices is currently the "holy grail" within the μTAS research community. While a wide variety of highly sophisticated chips which could potentially revolutionize healthcare, biology, chemistry and all related disciplines are increasingly being demonstrated, very few chips are or can be adopted by the market and reach the end-users. The major inhibition factor lies in the lack of an established commercial manufacturing technology. The lab-on-printed circuit board (lab-on-PCB) approach, while suggested many years ago, only recently has re-emerged as a very strong candidate, owing to its inherent upscaling potential: the PCB industry is well established all around the world, with standardized fabrication facilities and processes, but commercially exploited currently only for electronics. Owing to these characteristics, complex μTASs integrating microfluidics, sensors, and electronics on the same PCB platform can easily be upscaled, provided more processes and prototypes adapted to the PCB industry are proposed. In this article, we will be reviewing for the first time the PCB-based prototypes presented in the literature to date, highlighting the upscaling potential of this technology. The authors believe that further evolution of this technology has the potential to become a much sought-after standardized industrial fabrication technology for low-cost μTASs, which could in turn trigger the projected exponential market growth of μTASs, in a fashion analogous to the revolution of Si microchips via the CMOS industry establishment.

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Simultaneous detection of lactate and glucose by integrated printed circuit board based array sensing chip

Cited by 24 publications

References 26 publications

Commercially Fabricated Printed Circuit Board Sensing Electrodes for Biomarker Electrochemical Detection: The Importance of Electrode Surface Characteristics in Sensor Performance

Commercially Fabricated Printed Circuit Board Sensing Electrodes for Biomarker Electrochemical Detection: The Importance of Electrode Surface Characteristics in Sensor Performance

Simple Fabrication of Flexible Biosensor Arrays Using Direct Writing for Multianalyte Measurement from Human Astrocytes

The lab-on-PCB approach: tackling the μTAS commercial upscaling bottleneck

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