Simultaneous determination of aflatoxin B₁and M₁in milk, fresh milk and milk powder by LC-MS/MS utilising online turbulent flow chromatography

Abstract

A novel, fully automated method based on dual-column switching using online turbulent flow chromatography followed by LC-MS/MS was developed for the determination of aflatoxin B1 and M1 in milk, fresh milk and milk powder samples. After ultrasound-assisted extraction, samples were directly injected into the chromatographic system and the analytes were concentrated on the clean-up loading column. Through purge switch, analytes were transferred to the analytical column for subsequent detection by mass spectrometry. Different types of TurboFlow(TM) columns, transfer flow rates and transfer times were optimised. Method limits of detection obtained for AFB1 and AFM1 were 0.05 μg kg(-1), and limits of quantification were 0.1 μg kg(-1). Recoveries of aflatoxin B1 and M1 were in range of 81.1-102.1% for all samples. Matrix effects of aflatoxin B1 and M1 were in range of 63.1-94.3%. The developed method was successfully used for the analysis of aflatoxin B1 and M1 in real samples.

Current Trends in Fully Automated On-Line Analytical Techniques for Beverage Analysis

“…So far, milk is one of the matrices most frequently analyzed by TFC, although this technique has also been employed for the determination of multiclass veterinary drugs (VDs) [82][83][84] mycotoxins [85] and UV-filters [86] Milk is a complex sample rich in lipids and proteins, therefore the extraction and sample clean up remain a challenge when other sample preparation techniques are employed. TFC offers a perceived advantage of enabling the direct injection of milk samples into a TFC column after a simple protein precipitation step.…”

Recent advances of online coupling of sample preparation techniques with ultra high performance liquid chromatography and supercritical fluid chromatography

“…In this study, the total analysis time was 14 min and the recoveries ranged from 78 to 116% for all the analytes. In the study of simultaneous determination of aflatoxin B1 and M1 in milk, fresh milk, and milk powder using online TFC‐UHPLC, the matrix effects were in the range of 63.1–94.3% . During the analysis of polyphenols in complex royal jelly products using online TFC‐UHPLC system, 12 compounds were detected in the royal jelly with the highest intensities compared with other direct injection methods .…”

Progress on Structured Biosensors for Monitoring Aflatoxin B1 From Biofilms: A Review

“…Traditionally, aflatoxin B1 (AFB1) detection is performed by thin-layer chromatography (TLC) (Var et al, 2007;Casoni et al, 2017), enzyme-linked immunosorbent assay (ELISA) (Lee et al, 2004), mass spectrometry (MS), gas chromatography (GC), liquid chromatography (LC) (Jin and Choi, 2007;Fan et al, 2015), and high-performance LC (HPLC) (Ghali et al, 2009). These detection assays benefit from having a high sensitivity and mature technology.…”