Simultaneous Determination of Aflatoxins, Ochratoxin A, and Zearalenone in Grains by New Immunoaffinity Column/Liquid Chromatography

Göbel, R.; Lusky, K.

doi:10.1093/jaoac/87.2.411

Cited by 66 publications

(19 citation statements)

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“…Toxicology program have reviewed hundreds of chemicals, mixtures, and natural products and then graded the cancer risk posed to humans (Abnet 2007). All analytical procedures include 3 steps: extraction, purification (if necessary), and determination (Gobel and Lusky 2004; Ren and others 2007).…”

Section: Mycotoxinsmentioning

confidence: 99%

Qualitative and Quantitative Analysis of Mycotoxins

Rahmani¹,

Selamat²,

Soleimany³

2009

Comp Rev Food Sci Food Safe

205

128

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Mycotoxin toxicity occurs at very low concentrations, therefore sensitive and reliable methods for their detection are required. Consequently, sampling and analysis of mycotoxins is of critical importance because failure to achieve a suitable verified analysis can lead to unacceptable consignments being accepted or satisfactory shipments unnecessarily rejected. The general mycotoxin analyses carried out in laboratories are still based on physicochemical methods, which are continually improved. Further research in mycotoxin analysis has been established in such techniques as screening methods with TLC, GC, HPLC, and LC-MS. In some areas of mycotoxin method development, immunoaffinity columns and multifunctional columns are good choices as cleanup methods. They are appropriate to displace conventional liquid-liquid partitioning or column chromatography cleanup. On the other hand, the need for rapid yes/no decisions for exported or imported products has led to a number of new screening methods, mainly, rapid and easy-to-use test kits based on immuno-analytical principles. In view of the fact that analytical methods for detecting mycotoxins have become more prevalent, sensitive, and specific, surveillance of foods for mycotoxin contamination has become more commonplace. Reliability of methods and well-defined performance characteristics are essential for method validation. This article covers some of the latest activities and progress in qualitative and quantitative mycotoxin analysis.

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Section: Mycotoxinsmentioning

confidence: 99%

Qualitative and Quantitative Analysis of Mycotoxins

Rahmani¹,

Selamat²,

Soleimany³

2009

Comp Rev Food Sci Food Safe

205

128

View full text Add to dashboard Cite

show abstract

“…It has been reported that all the AFs have a molar absorptivity of 20,000 cm 2 /mol exhibiting maximum absorption at 360 nm (Akbas & Ozdemir, ). Experimental data suggested that the detection limit of AFs can be improved by the selection of appropriate method for extraction and cleanup procedure (Ali et al, ; Göbel & Lusky, ). The sensitivity of UV system is not enough to detect AFs in trace levels (Alcaide‐Molina et al, ) since its limit of detection reaches up to only micro molar ranges (Couderc, Caussé, & Bayle, ).…”

Section: Detection Techniquesmentioning

confidence: 99%

Rapid and sensitive detection of mycotoxins by advanced and emerging analytical methods: A review

Singh

Mehta

2020

Food Science & Nutrition

151

108

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Quantification of mycotoxins in foodstuffs is extremely difficult as a limited amount of toxins are known to be presented in the food samples. Mycotoxins are secondary toxic metabolites, made primarily by fungal species, contaminating feeds and foods. Due to the presence in globally used grains, it is an unpreventable problem that causes various acute and chronic impacts on human and animal health. Over the previous few years, however, progress has been made in mycotoxin analysis studies. Easier techniques of sample cleanup and advanced chromatographic approaches have been developed, primarily high-performance liquid chromatography. Few extremely sophisticated and adaptable tools such as high-resolution mass spectrometry and gas chromatography-tandem MS/MS have become more important. In addition, Immunoassay, Advanced quantitative techniques are now globally accepted for mycotoxin analysis. Thus, this review summarizes these traditional and highly advance methods and their characteristics for evaluating mycotoxins. K E Y W O R D Sadvanced quantitative techniques, chromatography, immunological, mycotoxins, spectroscopy 2184 | SINGH aNd MEHTa ultraviolet, fluorescence, photomultiplier, ion mobility, and tandem mass spectrophotometry, fourier transforms near infrared, adsorptive stripping voltammetry, and their lower detection limits, and sensitivity of different types of matrice has been reviewed. For the determination of mycotoxins, traditional quantitative methods viz. chromatography, immunological, and the advanced methods viz. ultrahigh-performance liquid chromatography, fluorescence polarization immunoassay, nanoparticle-based methods, microfluidics, and phage display methods have been discussed extensively in this review.

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“…It revealed that the developed method had higher enrichment factor and similar LODs with the listed methods. 12,[33][34][35] Furthermore, the new derivative method has distinct advantages in terms of good sensitivity at the high group selectivity, simplicity of instrumentation, and cost-effectiveness.…”

Section: Analyses Of Real Samplesmentioning

confidence: 99%

Rapid and Effective Sample Cleanup based on Polyvinylpyrrolidone-Coated Magnetite Nanoparticles Coupled with New Pre-Column Derivatization for Determination of Aflatoxins B1, B2, G1 and G2 in Animal Feeds by High-Performance Liquid Chromatography with Fluorescence Detection

Zhao

Zhou

Zhang

et al. 2019

J. Braz. Chem. Soc.

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in animal feed samples based on magnetic solid-phase cleanup and novel pre-column derivatization has been developed. In this work, synthesized polyvinylpyrrolidone-coated Fe 3 O 4 magnetic nanoparticles were applied as adsorbents for rapid cleanup for extracts of animal feed samples. The purified extract was then derivatized with trifluoroacetic acid coupling ultraviolet irradiation which enabled the weakly fluorescent AFB 1 and AFG 1 transformed into their highly fluorescent hemiacetals derivatives. Afterward aflatoxins were separated and quantified by reverse phase liquid chromatography with fluorescence detection. The results indicated that four AFs presented excellent linear response with correlation coefficients > 0.996 and recoveries ranged from 86.7 to 108.9% with relative standard deviations (RSDs) from 3.6 to 6.4%. The limits of detection (LODs) were 0.0392, 0.2429, 0.0842, 0.4556 ng mL-1 for AFB 1 , AFB 2 , AFG 1 and AFG 2 , respectively.

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Simultaneous Determination of Aflatoxins, Ochratoxin A, and Zearalenone in Grains by New Immunoaffinity Column/Liquid Chromatography

Cited by 66 publications

References 0 publications

Qualitative and Quantitative Analysis of Mycotoxins

Qualitative and Quantitative Analysis of Mycotoxins

Rapid and sensitive detection of mycotoxins by advanced and emerging analytical methods: A review

Rapid and Effective Sample Cleanup based on Polyvinylpyrrolidone-Coated Magnetite Nanoparticles Coupled with New Pre-Column Derivatization for Determination of Aflatoxins B1, B2, G1 and G2 in Animal Feeds by High-Performance Liquid Chromatography with Fluorescence Detection

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