Simultaneous Determination of Amiloride Hydrochloride and Hydrochlorothiazide in Pharmaceuticals by Derivative Spectrophotometry

Abstract

Method is established for simultaneous determination of Amiloride hydrochloride (Amilo) and hydrochlorothiazide in tablets. The method is based on employing normal and derivative spectrophotometry using the zero order via described by measuring the absorbance at 361 nm and the first order derivative spectra at peak 340 and valley 382 nm for (Amilo), while the zerocrossing method is applied for hydrochlorothiazide. The first order derivative absorption spectra at valleys 285 and 239 nm were used for hydrochlorothiazide. No interferences found between both determined and those of matrices. A good accuracy and precision of simultaneous determination of (Amilo), and hydrochlorothiazide were confirmed by statistical analysis. The percentage recovery of the individual drags under the established conditions is ranged from 97.79% to 101.5 %. Linearity is maintained within a wide concentration range from 2.00 mg/L to 40.00 mg/L for Amiloride hydrochloride and hydrochlorothiazide. The detection limit was 0.5 and 1.0 mg/L for Amiloride hydrochloride and hydrochlorothiazide respectively.

Simultaneous Determination of Paracetamol and Hyoscine-N- Butyl Bromide in Binary Mixture Using Derivative Spectrophotometry and Their Application for Pharmaceutical Samples

“…The combination of PAR and HYO are used for the relief of smooth muscle spasm (cramps) of the gastrointestinal and genitourinary system, it is effective in the treatment of recurrent crampy abdominal pain. [2] Various methods have been reported for the simultaneous determination of the combination of PAR and HYO in pharmaceutical formulations and biological fluids, including RP-TLC and HPLC [3][4][5] , and UV spectrophotometry [2] , Derivative spectrophotometry (DS) is widely applied for determination for inorganic ions [6,7] and organic analysis, toxicology and clinical analysis, analysis of pharmaceutical products [6][7][8][9][10][11], , amino acids and proteins, in analysis of food and in environmental chemistry. In general, the application of DS is not limited to any particular case or filed, but it can be used whenever quantitative or qualitative investigations of broad spectra are difficult.…”

“…drugs PAR and HYO solutions were prepared by two series. First series of mixture solutions were prepared by using a fixed concentration (25 μg.mL -1 ) of PAR with different concent-rations (0.5, 1, 2, 4, 6, 8, 10, 15, 20, and 25 μg mL -1 ) of HYO, While the second series of mixture contains a fixed concentration (0.5 μg mL -1 ) of HYO with different concentration (2,4,6,8,10,15,20, and 25 μg mL -1 ) of PAR. 3.…”

“…The linear equation, correlation coefficient and concentration range for the calibration curve are listed in Table (1). The results of the relative error % and recovery % for the determination of PAR in the mixture are listed in Tables (8) and (9). Fig.…”

Spectrophotometric Method for Simultaneous Determination of Amiloride and Hydrochlorothiazide in Combined Dosage Form

“…Inhibition of sodium reabsorption creates a negative voltage in the luminal membranes of principal cells, situated at the distal convoluted tubule and collecting duct. This negative voltage decreases the potassium and hydrogen ion secretion [2,3]. It is used in conjunction with diuretics to spare potassium loss.…”

Simultaneous Estimation and Forced Degradation Studies of Amiloride Hydrochloride and Furosemide in a Pharmaceutical Dosage Form Using Reverse-Phase High-Performance Liquid Chromatography Method

“…The thorough literature survey reveals that few analytical methods such as RP-HPLC and UV methods are reported for simultaneous estimation of amiloride hydrochloride and furosemide in pharmaceutical dosage forms [3,6,7]. Thus, the present investigation was held out to acquire new, simple, accurate, rapid, and cost-effective stability indicating RP-HPLC method for the simultaneous estimation of amiloride hydrochloride and furosemide in pharmaceutical dosage form.…”