Simultaneous determination of amoxicillin and ranitidine in rat plasma by high-performance liquid chromatography

Li, Wei; Tan, Fengping; Zhao, Kang

doi:10.1016/j.jpba.2005.11.024

Cited by 31 publications

(15 citation statements)

References 26 publications

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“…In order to assure the quality of R . HCl containing pharmaceutical formulations, several methods have been developed for its determination, including high-performance liquid chromatography (HPLC) [1][2][3][4][5][6] , high-performance thin-layer chromatography and scanning densitometry 7 , differential pulse polarography 8 , amperometry 9 , capillary electrophoresis 10 , conductometry 11 , coulometry 12 , voltammetry 13 , potentiometry 14 , fluorimetry 15 , chemiluminescence 16 , AAS 17 . The official method described in the United States Pharmacopeia 18 is based on potentiometric titration of R .…”

Section: Ranitidine N-(2-{[(5-dimethylamino)methyl]-2-furanyl}-methymentioning

confidence: 99%

Spectrophotometric Method for Ranitidine Determination in Drugs Using Rhodamine B

Moldovan¹,

Aboul‐Enein²

2012

J. Chil. Chem. Soc.

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A new spectrophotometric method has been developed for the determination of ranitidine hydrochloride (R . HCl) in bulk drug and in tablet formulation. The proposed method involves the addition of a measured excess of bromate-bromide reagent in hydrochloric acid medium to R . HCl, followed by determination of residual bromine by reacting with a fixed amount of Rhodamine B (RB) and measuring the absorbance at λ max =557 nm. Beer's law was found in the range of 0.3-3.2 μg ml -1 of R . HCl, with a good correlation coefficient (r=0.9997). The limits of detection and quantification were calculated to be 0.08 μg ml -1 and 0.24mg ml -1 , respectively. The different experimental parameters affecting the development and stability of the proposed method were carefully studied and optimized. The validity of the proposed method tested by analyzing the pure and pharmaceutical formulations and compared well with those obtained by the official method, demonstrated good accuracy and precision.

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Section: Ranitidine N-(2-{[(5-dimethylamino)methyl]-2-furanyl}-methymentioning

confidence: 99%

Spectrophotometric Method for Ranitidine Determination in Drugs Using Rhodamine B

Moldovan¹,

Aboul‐Enein²

2012

J. Chil. Chem. Soc.

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“…One of the most frequently and successfully used methods for determination of AMX is HPLC [3][4][5][6][7]. This method suffers from some disadvantages such as requiring large amount of highly pure organic solvents, long equilibration times, and derivatizing treatment.…”

Section: Introductionmentioning

confidence: 99%

Highly sensitive colorimetric determination of amoxicillin in pharmaceutical formulations based on induced aggregation of gold nanoparticles

Akhond

Absalan

Ershadifar

2015

Spectrochimica Acta Part A: Molecular and Biomolecular Spectros

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“…An additional aim of derivatization is the introduction of chemical moieties that improve detection by liquid chromatography-fluorescence [5,9]. Methods involving high-performance liquid chromatography (HPLC) using ultraviolet (UV) [10], mass spectrometry (MS) [11], or diode array detection (DAD) [12] were reported for the determination of amoxicillin and another penicillin drugs in milk [13], fish [7], urine [14], human plasma [12], and animal plasma, e.g., rats [15], mouse [16], bovine [17], or pigs [18]. This paper describes a sensitive method for the determination of amoxicillin in poultry plasma.…”

Section: Introductionmentioning

confidence: 99%

Determination of amoxicillin in poultry plasma by high-performance liquid chromatography after formaldehyde derivation

Krasucka¹,

Kowalski²,

Osypiuk³

et al. 2015

Acta Chromatographica

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Summary.A high-performance liquid chromatographic (HPLC) method with fluorescence detection after precolumn formaldehyde derivation was developed to detect concentrations of amoxicillin (AMX) in poultry plasma. Proteins in plasma samples spiked with AMX were precipitated with a phosphate buffer and trichloroacetic acid. After precolumn treatment of the extraction product of AMX with formaldehyde under acidic and heating conditions, HPLC analysis with fluorescence (FL) detection at an excitation wavelength of 355 nm and an emission wavelength of 450 nm was performed. A mobile phase comprising acetonitrile and a buffer solution (0.05 M KH2PO4 pH = 5.6), which yielded AMX retention time 8.58 min, was suitable for detection of AMX. The calculated standard curve of the reaction product was linear, and the correlation coefficient was greater than 0.999. The limit of detection and quantification, the accuracy, and the precision were evaluated. Recoveries of spiked amoxicillin were >92%, with a coefficient of variation in the range of 0.35-0.89%. This method has been successfully applied to a pharmacokinetic study after oral administration of amoxicillin to poultry.

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Simultaneous determination of amoxicillin and ranitidine in rat plasma by high-performance liquid chromatography

Cited by 31 publications

References 26 publications

Spectrophotometric Method for Ranitidine Determination in Drugs Using Rhodamine B

Spectrophotometric Method for Ranitidine Determination in Drugs Using Rhodamine B

Highly sensitive colorimetric determination of amoxicillin in pharmaceutical formulations based on induced aggregation of gold nanoparticles

Determination of amoxicillin in poultry plasma by high-performance liquid chromatography after formaldehyde derivation

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