Simultaneous determination of byak-angelicin and oxypeucedanin hydrate in rat plasma by column-switching high-performance liquid chromatography with ultraviolet detection

Ishihara, Kazuhisa; Fukutake, Miwako; Asano, Takayuki; Mizuhara, Yasuharu; Wakui, Yoko; Yanagisawa, Toshihiko; Kamei, Hideo; Ohmori, Shigeru; Kitada, Mitsukazu

doi:10.1016/s0378-4347(00)00569-7

Cited by 22 publications

(21 citation statements)

References 9 publications

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“…Compounds 1, 3, 5 and 6 showed the typical characteristics of linear furanocoumarin in the several spectral data including 1 H and 13 C-NMR. Their physical or spectral data were compared with those reported in the literature leading to their identifications as isoimperatorin (1) (Bergendorff et al, 1997;Gu et al, 1990;Kozawa et al, 1981), imperatorin (3) (Bergendorff et al, 1997;Razdan et al, 1982;Kozawa et al, 1981), phellopterin (5) (Kwon et al, 1997;Kozawa et al, 1981) and oxypeucedanin hydrate (6) (Kozawa et al, 1981;Ishihara et al, 2001). Also, 1 H-and 13 C-NMR data of compounds 9 and 10 indicated the presence of a linear hydrofurancoumarin skeleton and a glucopyranosyl moiety.…”

Section: Resultsmentioning

confidence: 99%

In vitro GABA-transaminase inhibitory compounds from the root ofAngelica dahurica

et al. 2005

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The 80% aqueous MeOH extracts from the root of Angelica dahurica, found to inhibit the activities of GABA degradative enzymes GABA transaminase (GABA-T) and succinic semialdehyde dehydrogenase (SSADH), were fractionated using EtOAc, n-BuOH and H2O. Repeated column chromatography for the EtOAc and n-BuOH fractions led to the isolation of two new coumarins, oxypeucedanin hydrate-3''-butyl ether and isopraeroside IV along with six known coumarins, isoimperatorin, imperatorin, phellopterin, oxypeucedanin hydrate, nodakenin and 3'-hydroxymarmesinin, and two polyacetylenes, falcarindiol and octadeca-1,9-dien-4,6-diyn-3,8,18-triol. Of the isolated pure compounds, imperatorin and falcarindiol inactivated the GABA-T activities in both time- and concentration-dependent manners. The kinetic studies showed that imperatorin and falcarindiol reacted with the GABA-T with a second-order rate constant of 2.3 +/- 0.2 mm(-1) min(-1) and 1.5 +/- 0.1 mm(-1) min(-1), respectively. It is postulated that imperatorin and falcarindiol are able to elevate the neurotransmitter GABA levels in the central nervous system by an inhibitory action on the GABA degradative enzyme GABA-T.

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Section: Resultsmentioning

confidence: 99%

In vitro GABA-transaminase inhibitory compounds from the root ofAngelica dahurica

et al. 2005

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“…Their structures were identified by comparison of optical rotations, 1 H-and 13 C-NMR, DEPT, and MS data with the reported ones of the corresponding compounds. 13,[17][18][19][20][21] Among the purified compounds, 3 showed the strongest protective effect against lethality induced by LPS/D-GalN ( Table 2). Pretreatment of mice with 3 at doses of 3, 10, and 30 mg/kg increased survival rates to 40%, 80%, and 100%, respectively, compared with 20% in the control experiment, while pretreatment with dexamethasone as a positive control at doses of 3, 10, and 30 mg/kg increased survival rates to 60%, 100%, and 100%, respectively.…”

Section: Resultsmentioning

confidence: 99%

“…10 (1.97 g), and Fr. 11 (3 g 17,20,21) respectively. The structures of the four compounds are shown in Fig.…”

Section: General Proceduresmentioning

confidence: 99%

Agents Protecting against Sepsis from the Roots of Angelica dahurica

Song

Kim

et al. 2005

Biological & Pharmaceutical Bulletin

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“…Hesperidin from C. reticulata 12) was extracted at higher amount in pressurized decoction before 90 min, however, extraction time did not significantly influence the content variation. The amount of apigetrin, which was the extracted compound from A. rugosa 13) , was decreased after the peak extraction time, 60 min, both pressurized and non-pressurized decoctions without significance, but the difference between two kinds of decoctions was significant at 150…”

Section: Comparison Of Extraction Yield Tssc and Ph In Gjs Decoctionsmentioning

confidence: 95%

Investigation of difference of Gwakhyangjeonggi-san decoctions produced by different pressure levels and various extraction times

Kim¹,

Lee²,

Shin³

et al. 2014

Herbal Formula Science

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Objectives : Gwakhyangjeonggi-san (GJS) which consists of 13 herbal medicines has been used to treat gastrointestinal disorders caused by common cold. This study was performed to compare GJS decoctions produced using different pressure levels for various extraction times.Methods : Decoctions were prepared by the pressure levels of 0 kgf/cm 2 (non-pressurized) or 1 kgf/cm 2 (pressurized) for 30-180 min. The extraction yield, total soluble solid content (TSSC), and hydrogen ion concentration (pH) were measured, and the contents of the nine marker compounds were determined using high performance liquid chromatography.Results : The higher pressure and longer extraction time significantly increased TSSC value, while decreased the pH value. However, only extraction time affected the extraction yield of pressurized decoction. Variation of the amounts of chemical compounds was shown in pressurized and non-pressurized decoctions during extraction time. The result of regression analysis showed that pressure and extraction time can influence [1][2][3][4] .In the present study, the extraction yield, total soluble solids content (TSSC), hydrogen ion concentration (pH), and the content of marker compoud were compared through GJS decoctions Preparation of standard solutionsThe standard compounds were accurately weighed and dissolved in methanol to produce stock solutions. Each stock solution was diluted to make working solutions, which were used to construct calibration curves. Preparation of GJS decoctions and samplesThe herbal mixture of GJS (675 g corresponding to one formula set, 'Je' in Korean) were extracted at 100°C in water using a high-speed vacuum of each decoction was measured using a refractometer Commun. 2014) were applied to this study. Statistical analysesTwo-tailed t-tests and Dunnett's test were conducted for the two-group and the multi-group comparisons using Microsoft Excel (Microsoft, Redmond, WA, USA) and SYSTAT 10 (SPSS Inc., Chicago, IL, USA). Differences were considered significant at P < 0.05, P < 0.01, or P < 0.001.Regression analysis was performed through extraction yields, TSSC, pH, and the amount of Table 2. The nine marker compounds of GJS decoction, such as chlorogenic acid, caffeic acid, liquiritin, hesperidin, apigetrin, rosmarinic acid, oxypeucedanin hydrate, byakangelicin, and glycyrrhizin, were well separated on chromatograms by using the methods described above (Fig. 3).Chlorogenic acid and caffeic acid were mainly extracted from P. frutescens var. crispa and rosmarinic acid was extracted from both A. rugosa and P. frutescens var. crispa [8][9][10] . In the pressurized decoctions, the amounts of chlorogenic acid, caffeic acid, and rosmarinic acid were

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Simultaneous determination of byak-angelicin and oxypeucedanin hydrate in rat plasma by column-switching high-performance liquid chromatography with ultraviolet detection

Cited by 22 publications

References 9 publications

In vitro GABA-transaminase inhibitory compounds from the root ofAngelica dahurica

In vitro GABA-transaminase inhibitory compounds from the root ofAngelica dahurica

Agents Protecting against Sepsis from the Roots of Angelica dahurica

Investigation of difference of Gwakhyangjeonggi-san decoctions produced by different pressure levels and various extraction times

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