Simultaneous determination of diosmin and diosmetin in human plasma by ion trap liquid chromatography–atmospheric pressure chemical ionization tandem mass spectrometry: Application to a clinical pharmacokinetic study

Campanero, Miguel Ángel; Escolar, M.; Pérez, Guiomar; García-Quetglas, E.; Sádaba, Belén; Azanza, J. R.

doi:10.1016/j.jpba.2009.09.012

Cited by 100 publications

(66 citation statements)

References 12 publications

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“…Diosmin is a naturally occurring flavonoid, abundant in the pericarp of various citrus fruits (Figure 4) [140]. Excessive production of free fatty acids (hyperglycaemia) has previously been shown to cause inflammation leading to mitochondrial DNA damage and pancreatic cell malfunctioning [141].…”

Section: Natural Anti-diabetic/inflammatory Bioactive Compounds Anmentioning

confidence: 99%

Natural Phyto-Bioactive Compounds for the Treatment of Type 2 Diabetes: Inflammation as a Target

et al. 2016

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Diabetes is a metabolic, endocrine disorder which is characterized by hyperglycemia and glucose intolerance due to insulin resistance. Extensive research has confirmed that inflammation is closely involved in the pathogenesis of diabetes and its complications. Patients with diabetes display typical features of an inflammatory process characterized by the presence of cytokines, immune cell infiltration, impaired function and tissue destruction. Numerous anti-diabetic drugs are often prescribed to diabetic patients, to reduce the risk of diabetes through modulation of inflammation. However, those anti-diabetic drugs are often not successful as a result of side effects; therefore, researchers are searching for efficient natural therapeutic targets with less or no side effects. Natural products’ derived bioactive molecules have been proven to improve insulin resistance and associated complications through suppression of inflammatory signaling pathways. In this review article, we described the extraction, isolation and identification of bioactive compounds and its molecular mechanisms in the prevention of diabetes associated complications.

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Section: Natural Anti-diabetic/inflammatory Bioactive Compounds Anmentioning

confidence: 99%

Natural Phyto-Bioactive Compounds for the Treatment of Type 2 Diabetes: Inflammation as a Target

et al. 2016

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“…4,[8][9][10] A powerful free-radical scavenger, 11 diosmin is a natural flavone glycoside readily obtained by dehydrogenation of the corresponding flavanone glycoside hesperidin; it is also abundant in the pericarp of various citrus fruits. 12,13 Diosmin has been reported to be effective in the prevention of I/Rinduced intestinal injury, and in tissue damage in the kidney, liver, intestine, cheek pouch, and brain. [14][15][16][17][18] However, there are few studies 18 on the protective effect of diosmin against retinal damage caused by I/R.…”

Section: Introductionmentioning

confidence: 99%

Diosmin Protects Rat Retina from Ischemia/Reperfusion Injury

Tong

Zhang

Gong

et al. 2012

Journal of Ocular Pharmacology and Therapeutics

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“…Previous pharmacokinetic investigations showed that only the aglycone DIOS can enter the bloodstream (no diosmin was detected in patent's blood plasma) [23,24]. After administration of 450 mg oral dose of diosmin, about 1.0-1.5 lM peak concentrations of DIOS were reported in the plasma (studied in healthy human volunteers) [24]. The above listed facts support that additional investigations are needed to explore further potential biochemical effects of DIOS.…”

Section: Introductionmentioning

confidence: 93%

“…In addition, there have been described several other positive effects attributed to diosmin in recently published studies both in vitro and in vivo, indicating a complex mechanism of action [17][18][19][20][21][22]. Previous pharmacokinetic investigations showed that only the aglycone DIOS can enter the bloodstream (no diosmin was detected in patent's blood plasma) [23,24]. After administration of 450 mg oral dose of diosmin, about 1.0-1.5 lM peak concentrations of DIOS were reported in the plasma (studied in healthy human volunteers) [24].…”

Section: Introductionmentioning

confidence: 99%

Flavonoid diosmetin increases ATP levels in kidney cells and relieves ATP depleting effect of ochratoxin A

Poór

Veres

Jakus

et al. 2014

Journal of Photochemistry and Photobiology B: Biology

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a b s t r a c tDiosmetin (DIOS) is a flavone aglycone commonly occurring in citrus species and olive leaves, in addition it is one of the active ingredients of some medications. Based on both in vitro and in vivo studies several beneficial effects are attributed to DIOS but the biochemical background of its action seems to be complex and it has not been completely explored yet. Previous investigations suggest that most of the flavonoid aglycones have negative effect on ATP synthesis in a dose dependent manner. In our study 17 flavonoids were tested and interestingly DIOS caused a significant elevation of intracellular ATP levels after 6-and 12-h incubation in MDCK kidney cells. In order to understand the mechanism of action, intracellular ATP and protein levels, ATP/ADP ratio, cell viability and ROS levels were determined after DIOS treatment. In addition, impacts of different enzyme inhibitors and effect of DIOS on isolated rat liver mitochondria were also tested. Finally, the influence of DIOS on the ATP depleting effect of the mycotoxin, ochratoxin A was also investigated. Our major conclusions are the followings: DIOS increases intracellular ATP levels both in kidney and in liver cells. Inhibition of glycolysis or citric acid cycle does not decrease the observed effect. DIOS-induced elevation of ATP levels is completely abolished by the inhibition of ATP synthase. DIOS is able to completely reverse the ATP-depleting effect of the mycotoxin, ochratoxin A. Most probably the DIOS-induced impact on ATP system does not originate from the antioxidant property of DIOS. Based on our findings DIOS may be promising agent to positively influence ATP depletion caused by some metabolic poisons.

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Simultaneous determination of diosmin and diosmetin in human plasma by ion trap liquid chromatography–atmospheric pressure chemical ionization tandem mass spectrometry: Application to a clinical pharmacokinetic study

Cited by 100 publications

References 12 publications

Natural Phyto-Bioactive Compounds for the Treatment of Type 2 Diabetes: Inflammation as a Target

Natural Phyto-Bioactive Compounds for the Treatment of Type 2 Diabetes: Inflammation as a Target

Diosmin Protects Rat Retina from Ischemia/Reperfusion Injury

Flavonoid diosmetin increases ATP levels in kidney cells and relieves ATP depleting effect of ochratoxin A

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