Simultaneous determination of flavanones, hydroxycinnamic acids and alkaloids in citrus fruits by HPLC-DAD–ESI/MS

He, Dongxiu; Shan, Yang; Wu, Yuehui; Liu, Guozhu; Chen, Bin; Yao, Shouzhuo

doi:10.1016/j.foodchem.2010.12.109

Cited by 98 publications

(62 citation statements)

References 34 publications

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“…In this study, we found that the major compounds of FME were neoeriocitrin and poncirin, of which the highest was neoeriocitrin. This is different from the reports about citrus fruits extracts, which showed that the major flavonoids are naringin, hesperidin and neohesperidin [34], [35]. This result is consistent with the report that the phenolic composition of the genus Fortunella is different from that of the genus Citrus , even though the two genera are closely related in taxonomy [36].…”

Section: Discussionsupporting

confidence: 52%

Effects of Fortunella margarita Fruit Extract on Metabolic Disorders in High-Fat Diet-Induced Obese C57BL/6 Mice

Tan

Ding

et al. 2014

PLoS ONE

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IntroductionObesity is a nutritional disorder associated with many health problems such as dyslipidemia, type 2 diabetes and cardiovascular diseases. In the present study, we investigated the anti-metabolic disorder effects of kumquat (Fortunella margarita Swingle) fruit extract (FME) on high-fat diet-induced C57BL/6 obese mice.MethodsThe kumquat fruit was extracted with ethanol and the main flavonoids of this extract were analyzed by HPLC. For the preventive experiment, female C57BL/6 mice were fed with a normal diet (Chow), high-fat diet (HF), and high-fat diet with 1% (w/w) extract of kumquat (HF+FME) for 8 weeks. For the therapeutic experiment, female C57BL/6 mice were fed with high-fat diet for 3 months to induce obesity. Then the obese mice were divided into two groups randomly, and fed with HF or HF+FME for another 2 weeks. Body weight and daily food intake amounts were recorded. Fasting blood glucose, glucose tolerance test, insulin tolerance test, serum and liver lipid levels were assayed and the white adipose tissues were imaged. The gene expression in mice liver and brown adipose tissues were analyzed with a quantitative PCR assay.ResultsIn the preventive treatment, FME controlled the body weight gain and the size of white adipocytes, lowered the fasting blood glucose, serum total cholesterol (TC), serum low density lipoprotein cholesterol (LDL-c) levels as well as liver lipid contents in high-fat diet-fed C57BL/6 mice. In the therapeutic treatment, FME decreased the serum triglyceride (TG), serum TC, serum LDL-c, fasting blood glucose levels and liver lipid contents, improved glucose tolerance and insulin tolerance. Compared with the HF group, FME significantly increased the mRNA expression of PPARα and its target genes.ConclusionOur study suggests that FME may be a potential dietary supplement for preventing and ameliorating the obesity and obesity-related metabolic disturbances.

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Section: Discussionsupporting

confidence: 52%

Effects of Fortunella margarita Fruit Extract on Metabolic Disorders in High-Fat Diet-Induced Obese C57BL/6 Mice

Tan

Ding

et al. 2014

PLoS ONE

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“…Besides its role as a biogenic amine, octopamine has been identified as an important active component in herbal products of Citrus aurantium [8] and other citrus species [9,10]. The fruit of C. aurantium , commonly known as bitter orange, is sometimes used as a food, but it is more widely ingested as a medicinal or dietary supplement.…”

Section: Introductionmentioning

confidence: 99%

Adrenergic Metabolic and Hemodynamic Effects of Octopamine in the Liver

Oliveira

Paula

Comar

et al. 2013

IJMS

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The fruit extracts of Citrus aurantium (bitter orange) are traditionally used as weight-loss products and as appetite suppressants. A component of these extracts is octopamine, which is an adrenergic agent. Weight-loss and adrenergic actions are always related to metabolic changes and this work was designed to investigate a possible action of octopamine on liver metabolism. The isolated perfused rat liver was used to measure catabolic and anabolic pathways and hemodynamics. Octopamine increased glycogenolysis, glycolysis, oxygen uptake, gluconeogenesis and the portal perfusion pressure. Octopamine also accelerated the oxidation of exogenous fatty acids (octanoate and oleate), as revealed by the increase in 14CO2 production derived from 14C labeled precursors. The changes in glycogenolysis, oxygen uptake and perfusion pressure were almost completely abolished by α1-adrenergic antagonists. The same changes were partly sensitive to the β-adrenergic antagonist propranolol. It can be concluded that octopamine accelerates both catabolic and anabolic processes in the liver via adrenergic stimulation. Acceleration of oxygen uptake under substrate-free perfusion conditions also means acceleration of the oxidation of endogenous fatty acids, which are derived from lipolysis. All these effects are compatible with an overall stimulating effect of octopamine on metabolism, which is compatible with its reported weight-loss effects in experimental animals.

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“…There are many different methods used for the analysis of flavonoids in sweet orange peel [6], citrus fruits [1,4,7], pharmaceutical formulations [2], orange juice and orange wine [8], grapefruit [9][10][11], in food supplements [12] including high performance liquid chromatography, capillary electrophoresis with different detectors, UV-visible, diode array detection (DAD), mass spectrometry and electrochemical detectors. This paper represents fast method for determination of naringin and hesperidin in commercial and natural citrus fruit juices by HPLC with simple sample preparation.…”

Section: Introductionmentioning

confidence: 99%

Fast Determination of Naringin and Hesperidin in Natural and Commercial Citrus Juices by HPLC Method

Büyüktuncel¹

2017

Asian J. Chem.

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A reversed-phase high performance chromatographic method for simultaneous determination of naringin and hesperidin in natural and commercial citrus juices was developed. The separation was achieved on Zorbax Eclipse XDB C18 column (4.6 mm × 250 mm, 5 µm) using a acetonitrile:water:formic acid (21:78.8:0.2) solution at pH 2.5 as a mobile phase under isocratic conditions. Analysis was run at a flow rate of 1 mL min -1 with a 15 min total run time at room temperature. The detection wavelength was set at 280 nm. Linearity of calibration, accuracy, precision were examined as parts of the method validation. The optimized method was applied to analysis of natural and commercial citrus juices. Commercial fruit juices contained lower amount of naringin and hesperidin compared with natural fruit juices.

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Simultaneous determination of flavanones, hydroxycinnamic acids and alkaloids in citrus fruits by HPLC-DAD–ESI/MS

Cited by 98 publications

References 34 publications

Effects of Fortunella margarita Fruit Extract on Metabolic Disorders in High-Fat Diet-Induced Obese C57BL/6 Mice

Effects of Fortunella margarita Fruit Extract on Metabolic Disorders in High-Fat Diet-Induced Obese C57BL/6 Mice

Adrenergic Metabolic and Hemodynamic Effects of Octopamine in the Liver

Fast Determination of Naringin and Hesperidin in Natural and Commercial Citrus Juices by HPLC Method

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