Simultaneous determination of histamine and polyamines by capillary zone electrophoresis with 4-fluor-7-nitro-2,1,3-benzoxadiazole derivatization and fluorescence detection

Zhang, Li-Yao; Tang, Xuhai; Sun, Mingwei

doi:10.1016/j.jchromb.2005.03.033

Cited by 34 publications

(16 citation statements)

References 53 publications

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“…Alternatively, Krausova et al [27,28] extended the application of a MEKC with UV detection to the analysis of biogenic amines in numerous meat matrices, including young bull, cow, pig and chicken livers. A new procedure based on the use of CZE with lamp-induced fluorescence detection and 4-fluor-7-nitro-2,1,3-benzoxadiazole as derivatization reagent was developed to determine histamine and polyamines including spermine, spermidine, diaminopropane, putrescine, cadaverine and diaminohexane [29]. A detection limit for the amines (10 nM) comparable to other CE analytical methods without using laser as the excitation radiation was achieved.…”

Section: Amino Acids Biogenic Amines and Nitrosaminesmentioning

confidence: 99%

Recent advances in the application of capillary electromigration methods for food analysis

Cifuentes¹

2006

Electrophoresis

150

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Section: Amino Acids Biogenic Amines and Nitrosaminesmentioning

confidence: 99%

Recent advances in the application of capillary electromigration methods for food analysis

Cifuentes¹

2006

Electrophoresis

150

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“…In addition, reproducibility of CE is usually not as great as the other two techniques, mainly due to variation of EOF as a result of adsorption of solutes on the capillary wall. Because most biogenic amines are protonated (R-NH 3 1 under physiological conditions, they are cations and thus their separations by CZE are commonly carried out under acidic conditions in the presence of small EOF [17,18]. However, the separations of biogenic amines by CZE are sometimes unsuccessful, especially because the biogenic amines have low hydrophilic properties, similar dissociation constant (K a ) values, and the tendency to be adsorbed on the capillary wall.…”

Section: Introductionmentioning

confidence: 99%

Analysis of biologically active amines by CE

et al. 2006

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This paper provides an overview on the current status of the analysis of biogenic amines by CE. The basic CE separation and detection strategies for the analysis of biogenic amines are briefly described. CZE and MEKC that provide highly efficient and reproducible analysis of biogenic amines are particularly surveyed. With respect to the detection of biogenic amines, we focus on LIF, UV-visible absorption, electrochemiluminescence, and MS. Derivatization strategies, indirect methods, and on-line concentration techniques such as field-amplified sample stacking, sweeping, and use of polymer solution are described. To show the practicality of CE, we highlight currently developed techniques for the determinations of biogenic amines in biological samples, including foods, beverages, cerebrospinal fluids, urine, and single cells.

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“…After being thoroughly mixed, derivatization reaction was conducted at 601C for 1 min in a water bath under dark. Then, the mixture was diluted with the running buffer solution to quench the labeling reaction; of course, it was also directly applied to injection for CE without dilution [22]. Reagent blanks without the target analytes were treated in the same way.…”

Section: Nbd-f-labeled Epmentioning

confidence: 99%

Multi‐wavelength light emitting diode array as an excitation source for light emitting diode‐induced fluorescence detection in capillary electrophoresis

et al. 2010

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A multi-wavelength LED array was used as an excitation source for in-column fiber-optic LED-induced fluorescence for CE. The light source consisted of a multi-wavelength LED array consisting of three different LEDs (430, 450 and 480 nm), a focusing lens and a gradient index lens group. The LED beam was collimated and reshaped with the gradient index lens group for coupling the LED light source into a single-mode optical fiber. In addition, the luminance and stability of the LED light source was improved by powering the LED under constant current at enhanced voltages. The benefits of this system were demonstrated by the simultaneous determination of FITC-labeled L-asparagine (Ex/Em 488/520 nm), 4-fluoro-7-nitro-2,1,3-benzoxadiazole-labeled epinephrine (Ex/Em 468/530 nm) and 3-(4-carboxybenzoyl)-quinoline-2-carboxaldehyde-labeled L-leucine (Ex/Em 440/530 nm). Detection limits of L-asparagine, epinephrine and L-leucine were estimated to be 0.8 x 10(-9), 12.0 x 10(-8) and 4.0 x 10(-8) M (S/N=3), respectively. The RSDs (n=6) for migration time and peak area were better than 0.71 and 0.92%, respectively. The performance of the developed multi-wavelength LED excitation source was compared to the use of a single-wavelength LED and found to provide superior sensitivity for the three fluorophores used in this study.

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Simultaneous determination of histamine and polyamines by capillary zone electrophoresis with 4-fluor-7-nitro-2,1,3-benzoxadiazole derivatization and fluorescence detection

Cited by 34 publications

References 53 publications

Recent advances in the application of capillary electromigration methods for food analysis

Recent advances in the application of capillary electromigration methods for food analysis

Analysis of biologically active amines by CE

Multi‐wavelength light emitting diode array as an excitation source for light emitting diode‐induced fluorescence detection in capillary electrophoresis

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