Simultaneous determination of phenolics and alkaloids using ion exchange chromatography for sample preparation

Rensen, Irmgard van; Veit, Markus

doi:10.1002/pca.2800060302

Cited by 6 publications

(5 citation statements)

References 6 publications

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“…Presence of a free hydroxyl group in 13-hydroxylupanine decreased the elution efficiency of this compound from the SCX support. This result is comparable with the yields obtained during isolation of quinolizidine alkaloids by SPE from plants belonging to various species of the genus Genista (van Rensen and Veit, 1995). The low yield of 13-hydroxylupanine could be improved by increasing the volume of eluant, but even application of double amounts did not yield 100% recovery (data not presented).…”

Section: Resultssupporting

confidence: 79%

Application of solid phase extraction for profiling quinolizidine alkaloids and phenolic compounds in Lupinus albus

1997

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Solid phase extraction on strong cation exchanger (benzene sulphonic groups) and reverse phase (octadecyl groups) cartridges connected in series was used for a one-step isolation of quinolizidine alkaloids and phenolic compounds present in extracts from lupin seedlings. Both classes of compounds were further analysed by gas chromatography-mass spectrometry. This technique was used to analyse changes in the alkaloid and isoflavonoid profiles during the defence reaction of lupin plants evoked by abiotic (CuCl 2 ) and biotic (elicitor preparation from Colletotrichum lindemuthianum cell walls) factors.

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Section: Resultssupporting

confidence: 79%

Application of solid phase extraction for profiling quinolizidine alkaloids and phenolic compounds in Lupinus albus

1997

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“…2. In addition, the optimized SPE protocol using MCX cartridges increased the recovery of alkaloids during the extraction by 15-30% compared to the strong cation exchanger (benzene sulphonic groups) and reverse phase [51,52].…”

Section: Resultsmentioning

confidence: 99%

Natural alkaloids from narrow-leaf and yellow lupins transfer to soil and soil solution in agricultural fields

Hama

Strobel

2020

Environ Sci Eur

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Background Lupin is a promising legume crop, belongs to the Fabaceae (or Leguminosae) family. Lupin production for traditional and functional foods or animal feed is limited, due to the content of toxic quinolizidine (QA)s and indole alkaloids (IA)s. These compounds may not only pose a risk to humans and animals through food consumption, but may also affect soil and aquatic ecosystems. Field experiments were conducted to study the alkaloids content in both narrow-leaved or blue (L. angustifolius) and yellow (L. luteus) lupin plant tissue during a full growing season and understand the environmental fate of alkaloids in soil and water. Suction cups were used to collect soil pore water (soil solution) at four depths: 10, 25, 50 and 70 cm. A full protocol for sample preparation and UPLC–MS/MS quantification of alkaloids in plant, soil and water was developed. Results During the field experiments the alkaloids in the plant tissues increased, at the harvest stage the content was highest with 21.4 and 24.6 mg/kg dry weight (dw) for blue and yellow lupin, respectively. In soil, alkaloids quantified during the growing season (max concentration was 1.3 × 102 µg/kg dw) and even detected after harvest (0.2 µg/kg dw). In soil pore water samples, alkaloids were not detected during summer, but the concentrations increased to 9.8 × 102 and 1.5 × 103 ng/L for blue and yellow, respectively, in September when autumn precipitation began. Conclusions The results show the amount of alkaloids transferred from plant tissue into soil and soil pore water estimated to be on average 0.016% and 0.005% in soil and soil pore water, respectively. Alkaloids leached from topsoil to subsoil layers; the concentrations decline with soil depth. This study demonstrates that alkaloids are mobile compounds in the soil environments, thus lupin production may affect soil or aquatic ecosystems, and reduce water quality.

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“…Solid-phase reagents (SSR) are often used to facilitate and speed-up purification procedures in organic synthesis, especially in combinatorial chemistry (Flynn, 1999; Solinas and Taddei, 2007). However, application of SSR to natural products research has been limited (Alexandratos, 2009; Vanrensen and Veit, 1995; Zhao et al, 2008). Using this approach, we were able to isolate and identify three new nitrogenous compounds from ginger that were named [6]-, [8]-, and [10]-zingerines, as they are 5-(6-amino-9 H -purin-9-yl) analogs of [6]-, [8]-, and [10]-gingerols respectively.…”

Section: Introductionmentioning

confidence: 99%

Identification of unprecedented purine-containing compounds, the zingerines, from ginger rhizomes (Zingiber officinale Roscoe) using a phase-trafficking approach

et al. 2011

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Simultaneous determination of phenolics and alkaloids using ion exchange chromatography for sample preparation

Cited by 6 publications

References 6 publications

Application of solid phase extraction for profiling quinolizidine alkaloids and phenolic compounds in Lupinus albus

Application of solid phase extraction for profiling quinolizidine alkaloids and phenolic compounds in Lupinus albus

Natural alkaloids from narrow-leaf and yellow lupins transfer to soil and soil solution in agricultural fields

Identification of unprecedented purine-containing compounds, the zingerines, from ginger rhizomes (Zingiber officinale Roscoe) using a phase-trafficking approach

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