Simultaneous determination of plasmatic phytosterols and cholesterol precursors using gas chromatography–mass spectrometry (GC–MS) with selective ion monitoring (SIM)

Ahmida, H S Mohamed; Bertucci, Pierfrancesco; Franzò, Letizia; Massoud, Renato; Cortese, Claudio; Lala, A.; Federici, Giorgio

doi:10.1016/j.jchromb.2006.05.024

Cited by 91 publications

(59 citation statements)

References 20 publications

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“…Serum cholesteryl ester transfer protein (CETP) levels were determined by a specific enzymelinked immunosorbent assay (Kiyohara et al 1998). Serum levels of sterol, including those of sitosterol, lathosterol and campesterol, were determined using gas-liquid chromatography-mass spectrometry (Ahmida et al 2006).…”

Section: Biochemical Analysismentioning

confidence: 99%

Infantile Cases of Sitosterolaemia with Novel Mutations in the ABCG5 Gene: Extreme Hypercholesterolaemia is Exacerbated by Breastfeeding

et al. 2015

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Few data exists regarding the clinical impact of breastfeeding in infantile sitosterolaemic cases. We report four Japanese infantile cases of sitosterolaemia, an extremely rare inherited disease characterised by increased serum levels of plant sitosterol, presenting with severe hypercholesterolaemia and systemic xanthomas exacerbated by breastfeeding. In these four cases, genetic analyses were performed for low-density lipoprotein (LDL) receptor, proprotein convertase subtilisin/kexin type 9 (PCSK9), LDL receptor adaptor protein 1 and ATP-binding cassette (ABC) subfamily G member 5 and 8 (ABCG5 and ABCG8) genes. We assessed their clinical manifestations, including responsiveness to a variety of treatments, especially to weaning from breastfeeding and use of ezetimibe. Two pairs of mutations in the ABCG5 gene in each case, including two novel mutations (c.130C>T or p.Ser44Ala and c.1813_1817delCTTTT or p.Pro558GlufsX14) and two known mutations (c.1306G>A or p.Arg389His and c.1336C>T or p.Arg446X), were identified. Significant reductions in cholesterol levels were obtained by means of weaning from breastfeeding alone. Substantial reductions in sitosterol levels, without any apparent side effects, were observed with ezetimibe. In conclusion, we have identified infantile Japanese sitosterolaemic subjects with extreme hypercholesterolaemia exacerbated by breastfeeding. Their unique response to weaning from breastfeeding, as well as to use of ezetimibe, could provide insights into the metabolic basis of sterols in humans.

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Section: Biochemical Analysismentioning

confidence: 99%

Infantile Cases of Sitosterolaemia with Novel Mutations in the ABCG5 Gene: Extreme Hypercholesterolaemia is Exacerbated by Breastfeeding

et al. 2015

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“…Several sterol-type compounds circulate along with cholesterol in serum (23 ), and previous studies have shown that ␤-sistosterol, campesterol, desmosterol, and lathosterol interfere with AK measurement of cholesterol (4 ). Plant sterols such as ␤-sitosterol and campesterol in serum originate from oral consumption, whereas a few endogenous sterols such as lathosterol, desmosterol, and ergosterol are precursors in the biosynthesis of cholesterol and vitamin D (24,25 ).…”

Section: Interference Analysismentioning

confidence: 99%

Proposed Serum Cholesterol Reference Measurement Procedure by Gas Chromatography–Isotope Dilution Mass Spectrometry

et al. 2011

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“…Ahmida et al (2006) reported that LODs of campesterol and β-sitosterol in human plasma using GC-MS-SIM were 0.20 and 0.083 mg kg -1 , respectively. Santos et al (2007) reported that LODs of campesterol, stigmasterol and β-sitosterol in milk and yoghurt using GC-MS were 0.1 mg kg -1 .…”

Section: Validation Of the Methods Linearity (Calibration Curvementioning

confidence: 99%

Determination of Main Plant Sterols in Turkish Bread Wheat (Triticum aestivum L.) by GC-MS

Erdem

Tosun

Akkbik

et al. 2017

Turkish JAF Sci.Tech.

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Plant sterols are belong to triterpenes family of natural products which includes more than 200 different types of plant sterols and more than 4000 other types of triterpenes. The optimization of method, specially the derivatization step as well as the corresponding analytical validation, is the main goal of this study. The optimum temperature, time and reagent volume of derivatization step were obtained at 60°C, 60 minutes and 50 µL, respectively. A rapid and sensitive gas chromatographic-mass spectrometric method was developed and validated for quantitative analysis of the most common plant sterols (β-sitosterol, campesterol and stigmasterol) in 20 Turkish bread wheat cultivars using GC-MS-SIM. Separation of β-cholestanol (I.S), campesterol, stigmasterol and β-sitosterol was achieved on Rxi (5Sil MS) column (60 m×0.25 mm). The limits of detection for β-sitosterol, campesterol and stigmasterol were 0.074, 0.054 and 0.064 mg kg

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Simultaneous determination of plasmatic phytosterols and cholesterol precursors using gas chromatography–mass spectrometry (GC–MS) with selective ion monitoring (SIM)

Cited by 91 publications

References 20 publications

Infantile Cases of Sitosterolaemia with Novel Mutations in the ABCG5 Gene: Extreme Hypercholesterolaemia is Exacerbated by Breastfeeding

Infantile Cases of Sitosterolaemia with Novel Mutations in the ABCG5 Gene: Extreme Hypercholesterolaemia is Exacerbated by Breastfeeding

Proposed Serum Cholesterol Reference Measurement Procedure by Gas Chromatography–Isotope Dilution Mass Spectrometry

Determination of Main Plant Sterols in Turkish Bread Wheat (Triticum aestivum L.) by GC-MS

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