Simultaneous determination of praziquantel, pyrantel embonate, febantel and its active metabolites, oxfendazole and fenbendazole, in dog plasma by liquid chromatography/mass spectrometry

Abstract

A liquid chromatography-electrospray-mass spectrometry method (LC/MS) has been developed and validated for determination of praziquantel (PZQ), pyrantel (PYR), febantel (FBT), and the active metabolites fenbendazole (FEN) and oxfendazole (OXF), in dog plasma, using mebendazole as internal standard (IS). The method consists of solid-phase extractions on Strata-X polymeric cartridges. Chromatographic separation was carried out on a Phenomenex Gemini C6 -Phenyl column using binary gradient elution containing methanol and 50 mm ammonium-formate (pH 3). The method was linear (r(2) ≥ 0.990) over concentration ranges of 3-250 ng/mL for PYR andFEB, 5-250 ng/mL for OXF and FEN, and 24-1000 ng/mL for PZQ. The mean precisions were 1.3-10.6% (within-run) and 2.5-9.1% (between-run), and mean accuracies were 90.7-109.4% (within-run) and 91.6-108.2% (between-run). The relative standard deviations (RSD) were <9.1%. The mean recoveries of five targeted compounds from dog plasma ranged from 77 to 94%.The new LC/MS method described herein was fully validated and successfully applied to the bioequivalence studies of different anthelmintic formulations such as tablets containing PZQ, PYR embonate and FBT in dogs after oral administration.

Determination of Praziquantel in Sparus aurata L. after Administration of Medicated Animal Feed

“…Given the use of chemotherapeutic agents in aquaculture systems, it is very important to develop rapid, simple and sensitive analytical methods able to determine and quantify traces of these drugs in edible parts of the fish. In the literature, there are many studies describing PZQ analysis in different matrices [4][5][6]. Moreover, several studies report the quantification of PZQ in food [7][8][9][10][11][12], but no study describes the determination of PZQ in gilthead sea bream (Sparus aurata L.) after in-feed treatment.…”

Ultrasensitive detection of praziquantel in sea bass (Lateolabrax japonicus) using a lateral flow immunochromatographic assay

“…Therefore, it is very important to monitor the drug residues in food and aquatic products using an easy and fast method. Many methods to detect PZQ have been described, including thin-layer chromatography (Pavlovic et al, 2019), high-performance liquid chromatography (Dey et al, 2017;Guerrero Garduno et al, 2016;Zrncic et al, 2014), and liquid chromatography-tandem mass spectrometry (Hui et al, 2019;Jian-Ping et al, 2019;Klausz et al, 2015;Ming-Ming et al, 2017). These methods are mainly used because they are highly sensitive, but the methods are time-consuming, expensive, and need professional technicians to run them (Li et al, 2020;Wang et al, 2020;Wu et al, 2020).…”

RP- HPLC and TLC- densitometric methods for determination of oxfendazole in the presence of its alkali -induced degradation product

“…2 Different analytical techniques were applied for quantitative estimation of oxfendazole including potentiometric titration, 3,4 radio immunoassay, 5 spectrophotometric methods 6 and HPLC methods even alone or in presence of other compounds. [7][8][9][10][11][12][13][14][15][16][17] Oxfendazole is an amide group containing compound that made it highly sensitive to hydrolytic degradation in basic conditions with the production of the degradation product 2-amino-5-Phenylsulfinyl benzimidazole. There is no stability indicating analytical methods were reported for determination of oxfendazole in presence of its degradation product.…”