Simultaneous Determination of Sweeteners and Preservatives in Preserved Fruits by Micellar Electrokinetic Capillary Chromatography

Abstract

A micellar electrokinetic capillary method for the simultaneous determination of the sweeteners dulcin, aspartame, saccharin, and acesulfame-K and the preservatives sorbic acid; benzoic acid; sodium dehydroacetate; and methyl-, ethyl-, propyl-, isopropyl-, butyl-, and isobutyl-p-hydroxybenzoate in preserved fruits is developed. These additives are ion-paired and extracted using sonication followed by solid-phase extraction from the sample. Separation is achieved using a 57-cm fused-silica capillary with a buffer comprised of 0.05 M sodium deoxycholate, 0.02 M borate-phosphate buffer (pH 8.6), and 5% acetonitrile, and the wavelength for detection is 214 nm. The average recovery rate for all sweeteners and preservatives is approximately 90% with good reproducibility, and the detection limits range from 10 to 25 microg/g. Fifty preserved fruit samples are analyzed for the content of sweeteners and preservatives. The sweeteners found in 28 samples was aspartame (0.17-11.59 g/kg) or saccharin (0.09-5.64 g/kg). Benzoic acid (0.02-1.72 g/kg) and sorbic acid (0.27-1.15 g/kg) were found as preservatives in 29 samples.

Removal of Emerging Contaminants by Fenton and UV-Driven Advanced Oxidation Processes

“…Alkyl esters of p-hydroxybenzoic acid (parabens) are widely used as ingredients of pharmaceuticals, shampoos, liquid bath admixtures, skin care products, dental care products, soaps, sun screen agents, and hair styling products. Parabens can also be found in some drugs and foodstuffs, to preserve them from microbial attack (Lin and Choong 1999;Lin et al 2000;Soni et al 2005;Gryglik et al 2009;Błedzka et al 2012;Błędzka et al 2014). There is very little solid evidence regarding the extent parabens are absorbed and the resulting effects on male reproduction (endocrine disruptors).…”

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“…The calibration curves exhibited good linearity in the range of 90-1220 µg/mL with r of 0.9996 for C, 80-1120 µg/mL with r of 0.9998 (DMC), and 80-1200 µg/mL with r of 0.9998 (BDMC). [28] Analysis of curcuminoids in some Curcuma genus which are indigenous to Indonesia, namely, C. manga Val. and v. Zijp, C. heyneana Val.…”

“…Curcumin was also found to increase intestinal lipase, sucrose, and maltase activity. [61,62] Lin et al [28] have reported that curcumin suppresses intestinal fibrosis by inhibition of PPARΓ-mediated epithelial-mesenchymal transition. Zhang et al [63] have carried out experiment on the protective effect of curcumin on TNBS-induced intestinal inflammation which is mediated through the JAK/STAT pathway and reported excellent results.…”

High performance liquid chromatographic determination of sodium benzoate, methylparaben and propylparaben as preservative components in nystatin suspensions

“…Sodium *Corresponding author. E-mail: yyamini@modares.ac.ir benzoate (SB) and alkyl esters of p-hydroxybenzoic acid (parabens) including methylparaben (MP) and propylparaben (PP) are usually used as preservatives to prevent foods, cosmetics, and pharmaceuticals from microbial and fungal attacks [3][4][5][6][7]. Their toxicity is generally low owing to fast hydrolysis in vivo to parent acid, which is rapidly conjugated and excreted [8].…”