Simultaneous determination of thiamphenicol, florfenicol and florfenicol amine in swine muscle by liquid chromatography–tandem mass spectrometry with immunoaffinity chromatography clean-up

Simultaneous determination of thiamphenicol, florfenicol and florfenicol amine in eggs by reversed-phase high-performance liquid chromatography with fluorescence detection

“…Comparison of the public methods to the present study; the LODs and LOQs of TAP, FF, and FFA were higher than other methods in the reports of Shen et al [23] and Luo et al [28], however, the present procedure of the extraction was more simple and low cost. The extracts were only purified by liquid-liquid extraction with ethyl acetate-acetonitrile-ammonium hydroxide (49:49:2, v/v), defatted with hexane.…”

“…Therefore, FFA was defined as one of the FF residue markers by many countries and organizations. There are various analytical methods have been reported for the determination of TAP, FF, and FFA in animal tissues, such as gas chromatography (GC) [15,16], liquid chromatography (LC) [17][18][19][20], GC-MS [21][22][23], LC-MS [24], and LC-MS/MS [25][26][27][28]. However, no public reports describe the method for the simultaneous determination of TAP, FF, and FFA in eggs right now.…”

Development and Validation of a Sensitive Indirect Competitive Enzyme-Linked Immunosorbent Assay for the Screening of Florfenicol and Thiamphenicol in Edible Animal Tissue and Feed

“…Therefore, the need for an effective residue monitoring programme in place for FF and TAP is clear. A number of analytical methods have been reported for the determination of TAP and FF, such as high performance liquid chromatography (HPLC) Hayes 2013;Xie et al 2011), liquid chromatography-tandem mass spectrometry (LC-MS) (Luo et al 2010;Rezende et al 2012;Sichilongo et al 2014a, b;Tao et al 2014), gas chromatography (GC) (Pfenning et al 2000;Zhang et al 2006), and gas chromatography-mass spectrometry (GC-MS) (Shen et al 2009). Although highly sensitive and reliable, these methods require well-equipped laboratories, trained personnel, and involve time-consuming sample preparation and therefore, they are not suitable for the rapid screening of numerous samples.…”

Recent advances and future trends in new materials for sample preparation

“…Luo et al [126] reported a method to quantify thiamphenicol, florfenicol and florfenicol amine using LC-ESI-MS/MS and a column based on pAbs employed to clean-up swine-muscle samples. The LODs were 0.4-4.0 µg kg -1 and the average recoveries were 85.2-98.8%.…”