Simultaneous determination of thiamphenicol, florfenicol and florfenicol amine in eggs by reversed-phase high-performance liquid chromatography with fluorescence detection

Xie, Kaizhou; Jia, Longfei; Yao, Yilin; Xu, Dong; Chen, Shuqing; Xie, Xiulan; Yan, Ping; Bao, Wenbin; Dai, Guojun; Wang, Jinyu; Liu, Zongping

doi:10.1016/j.jchromb.2011.06.027

Cited by 81 publications

(47 citation statements)

References 23 publications

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“…123 Ainda na China, foram encontrados florfenicol e florfenicol amina nas concentrações de 19 e 36 µg/kg, respectivamente, em apenas uma amostra das 50 amostras analisadas por HPLC-FL. 124 Alguns estudos avaliaram os níveis de resíduos de antimicrobianos e antibióticos em ovos após administração oral dos medicamentos em galinhas. Em um estudo, administrou-se enrofloxacina em galinhas por 4 dias (10 mg/kg pc/dia) e seus ovos foram recolhidos durante 15 dias para análise.…”

Section: Outros Paísesunclassified

Resíduos de medicamentos veterinários em leite e ovos

Pacheco-Silva¹,

Souza²,

Caldas³

2014

Quím. Nova

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VETERINARY DRUG RESIDUES IN MILK AND EGGS. The use of veterinary drugs in food producing animals may result in the presence of residues in foods, including milk and eggs. Immunoassay or microbiological tests are used to screen for residues, but chromatographic methods are needed to confirm positive results. In most methods, the sample is extracted with acetonitrile, submitted to clean up or directly analyzed by LC-MS/MS. Results of the Brazilian governmental monitoring programs from 2006 to 2011 have shown that the antiparasitic ivermectin was the drug most frequently found in milk. Residues in eggs are only monitored by one of the programs, and few studies have reported the incidence of veterinary drugs in this matrix in Brazil.Keywords: veterinary drugs; milk; eggs. INTRODUÇÃOMedicamentos são usados na medicina veterinária no tratamento e prevenção de doenças ou como promotores de crescimento. 1,2 Estes compostos compreendem uma variedade de classes químicas com ações terapêuticas diversas, incluindo antibiótica/antimicrobiana, antiparasitária, inseticida, fungicida e sedativa. 3 Os antimicrobianos (b-lactâmicos, aminoglicosídeos e tetraciclinas) e os antiparasitários (avermectinas, piretróides e organofosforados) são os produtos mais comercializados no Paraná para tratamento do rebanho leiteiro. 4 O uso de medicamentos veterinários em animais produtores de alimentos pode deixar resíduos nos alimentos, como carne, leite e ovos, cujos níveis não devem ultrapassar o Limite Máximo de Resíduos (LMR). O LMR é a concentração máxima de resíduo em um alimento de origem animal resultante do uso de um medicamento veterinário (expresso em mg/kg ou µg/kg, em peso fresco) que é recomendado para ser legalmente permitido ou reconhecido como sendo aceitável no alimento. 5 Estes limites são estabelecidos para garantir o uso adequado destes produtos, limitar a exposição e proteger a saúde dos consumidores dos alimentos provenientes de animais tratados. 5 A presença de resíduos em um alimento acima do LMR estabelecido indica que o produto não foi utilizado segundo as boas práticas de uso de medicamentos veterinários (BPMV), ou o uso de um produto não autorizado. 6 O Brasil não estabelece LMR para medicamentos veterinários, adotando aqueles recomendados pelo Mercosul, Codex Alimentarius, União Européia ou Estados Unidos. 7 A exposição humana a resíduos de medicamentos veterinários presentes nos alimentos pode causar efeitos adversos, incluindo reações alérgicas em indivíduos hipersensíveis 8 e câncer. 9,10 Adicionalmente, a exposição a antimicrobianos/antibióticos pode provocar o desenvolvimento de microrganismos resistentes, que dificulta a ação terapêutica destes medicamentos em indivíduos que consumiram alimentos de animais tratados. 11,12 Resíduos de medicamentos veterinários no leite podem também causar problemas tecnológicos nos processos de fermentação dos laticínios. 13,14 No Brasil, há dois programas nacionais que monitoram a presença de resíduos de medicamentos veterinários em produtos de origem animal. O Plano Nacional...

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Section: Outros Paísesunclassified

Resíduos de medicamentos veterinários em leite e ovos

Pacheco-Silva¹,

Souza²,

Caldas³

2014

Quím. Nova

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“…Considering that, it is necessary to establish a rapid, sensitive assay for the determination of CAP, TAP and FF residues in food samples. A number of analytical methods have been developed to detect CAP, TAP and FF; these include high-performance liquid chromatography (Xie et al, 2011), gas chromatographymass spectrometry (GC-MS) (Shen et al, 2009), liquid chromatography tandem mass spectrometry (LC-MS/MS) (Barreto, Ribeiro, Hoff, & Dalla Costa, 2016;Li, Hu, Huo, & Xu, 2006;Rezende, Filho, & Rocha, 2012), enzyme-linked immunosorbent assay (Fodey, Murilla, Cannavan, & Elliott, 2007;Liu et al, 2014; and lateral flow immunoassay (LFA) (Bai et al, 2013;Guo et al, 2015;Samsonova, Cannavan, & Elliott, 2012;Xu, Xu, Ma, Liu, et al, 2015). Compared with chromatographic methods, immunoassays are simple, rapid and do not require expensive equipment and professional operator.…”

Section: Introductionmentioning

confidence: 99%

Development of a quantitative fluorescence-based lateral flow immunoassay for determination of chloramphenicol, thiamphenicol and florfenicol in milk

Wang

Zheng

et al. 2017

Food and Agricultural Immunology

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A competitive lateral flow immunoassay (LFA) using fluorescent microspheres (FMs) as label was developed for the quantitative detection of chloramphenicol (CAP), thiamphenicol (TAP) and florfenicol (FF) in milk. The limit of detection (LOD) for CAP, TAP and FF in milk was 0.08, 0.8 and 1.9 μg L −1 , respectively. The recovery of intra and inter-assay ranged from 87.7% to 107.0% and 82.5% to 112.3%, with corresponding coefficient of variations less than 11.3% and 14.5%, respectively. The sensitivity of the FMs lateral flow assay (FMs-LFA) was comparable to those of the colloidal gold lateral flow assay. A quantitative comparison of the FMs-LFA and LC-MS/MS analysis of milk samples indicated good agreement between the two methods. The FMs-LFA can be used as a reliable, rapid and cost-effective method for food safety analysis. ARTICLE HISTORY

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“…Therefore, the need for an effective residue monitoring programme in place for FF and TAP is clear. A number of analytical methods have been reported for the determination of TAP and FF, such as high performance liquid chromatography (HPLC) Hayes 2013;Xie et al 2011), liquid chromatography-tandem mass spectrometry (LC-MS) (Luo et al 2010;Rezende et al 2012;Sichilongo et al 2014a, b;Tao et al 2014), gas chromatography (GC) (Pfenning et al 2000;Zhang et al 2006), and gas chromatography-mass spectrometry (GC-MS) (Shen et al 2009). Although highly sensitive and reliable, these methods require well-equipped laboratories, trained personnel, and involve time-consuming sample preparation and therefore, they are not suitable for the rapid screening of numerous samples.…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of a Sensitive Indirect Competitive Enzyme-Linked Immunosorbent Assay for the Screening of Florfenicol and Thiamphenicol in Edible Animal Tissue and Feed

Wang

Pan

et al. 2016

Food Anal. Methods

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To monitor the illegal use of florfenicol (FF) and thiamphenicol (TAP) in edible animal tissue and feed, a sensitive monoclonal antibody-based indirect competitive enzymelinked immunosorbent assay (ic-ELISA) has been developed with simple sample preparation and cleanup. The obtained monoclonal antibody (5F4) that has isotype IgG1 showed an IC 50 value of 0.21 μg L −1 for FF and 0.35 μg L −1 for TAP, respectively. It did not exhibit measurable cross-reactivity with other antibiotics. The limits of detection (LODs) for FF and TAP in a muscle matrix ranged from 0.07 to 0.14 μg kg −1 and in a feed matrix ranged from 2.9 to 5.2 μg kg −1 . The recoveries were 72.8 to 113.4 % with a coefficient of variation of less than 15 %. Good correlation between the ELISA and HPLC-MS/ MS results in the tissues tested demonstrated the reliability of our ic-ELISA. This ELISA is a useful tool for screening FF and TAP in edible animal tissue and feed.

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Simultaneous determination of thiamphenicol, florfenicol and florfenicol amine in eggs by reversed-phase high-performance liquid chromatography with fluorescence detection

Cited by 81 publications

References 23 publications

Resíduos de medicamentos veterinários em leite e ovos

Resíduos de medicamentos veterinários em leite e ovos

Development of a quantitative fluorescence-based lateral flow immunoassay for determination of chloramphenicol, thiamphenicol and florfenicol in milk

Development and Validation of a Sensitive Indirect Competitive Enzyme-Linked Immunosorbent Assay for the Screening of Florfenicol and Thiamphenicol in Edible Animal Tissue and Feed

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