Simultaneous determination of ticarcillin and clavulanate in rabbit serum and tissue cage fluid by liquid chromatography

Li, Chonghua; Geng, Qiuming; Nicolau, David P.; Nightingale, Charles H.

doi:10.1016/s1570-0232(03)00456-2

Cited by 10 publications

(8 citation statements)

References 24 publications

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“…The poor retention of clavulanic acid (p K a = 2.7) on a reversed‐phase column has already been published in the literature. Generally nonvolatile ion‐pairing reagents such as tri‐ and tetra‐alkylammonium salts4–7, 10, 11, 21, 22 and phosphate buffers8, 9, 12–16 are used in the mobile phase to lengthen the retention time, but these are not recommended for use in LC‐MS/MS. These compounds would precipitate in the LC‐MS interface, contaminate the ionization source and may cause ion suppression 34.…”

Section: Resultsmentioning

confidence: 99%

“…Moreover, by applying this gradient elution the compounds eluted as sharp peaks. This had the advantage of lowering the LOQ and LOD levels compared to other methods in the literature 4–19, 26. In addition, the column was well rinsed between consecutive sample injections.…”

Section: Resultsmentioning

confidence: 99%

“…Nevertheless, during the last decades, a lot of chromatographic assays were developed for the determination of clavulanic acid in plasma and urine. However, ultraviolet (UV) detection4–7 of clavulanic acid in biological matrices is difficult because of the low detection wavelength and consequently owing to the presence of endogenous compounds absorbing at this wavelength. Therefore, several methods make use of a precolumn derivatization with imidazole4, 8–13 or triazole14–16 combined with UV‐detection or fluorimetric detection after derivatization with benzaldehyde 17.…”

Section: Introductionmentioning

confidence: 99%

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Determination of clavulanic acid in calf plasma by liquid chromatography tandem mass spectrometry

et al. 2006

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A method for the quantification of clavulanic acid in calf plasma using high-performance liquid chromatography combined with electrospray ionization (ESI) mass spectrometry, operating in the negative ionization mode (LC-MS/MS), is presented. Sample preparation includes a simple and fast deproteinization with acetonitrile and a back-extraction of the acetonitrile with dichloromethane. Chromatography is performed on a reversed-phase PLRP-S polymeric column using 0.05% formic acid in water and acetonitrile. The limit of quantification is 25 ng/ml, which is lower than other published methods using ultraviolet (UV), fluorimetric or mass spectrometric detection. The limit of detection is calculated to be 3.5 ng/ml. The stability of clavulanic acid was demonstrated according to The Guidelines of Bioanalytical Method Validation of The Food and Drug Administration (FDA): freeze and thaw stability, short-term stability, long-term stability, stock solution stability and postpreparative stability. The method is used in a pharmacokinetic and bioequivalence study of amoxycillin/clavulanic acid formulations in calves.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Determination of clavulanic acid in calf plasma by liquid chromatography tandem mass spectrometry

et al. 2006

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“…Such a wavelength switch technique was also successfully applied in our laboratory to simultaneously determine another combination of β-lactam/ β-lactamase inhibitor, i.e. ticarcillin/clavulanate, in rabbit serum and TCF (Li et al, 2003).…”

Section: Resultsmentioning

confidence: 99%

Simultaneous analysis of piperacillin and tazobactam in rabbits: application to pharmacokinetic study

Xuan

et al. 2005

Biomed. Chromatogr.

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A simple and rapid assay is developed for the simultaneous analysis of piperacillin and tazobactam in rabbit serum and tissue cage fluid (TCF). To eliminate endogenous interferences, a wavelength switch technique was applied, in which the programmable UV detector changed the monitoring wavelength from 218 to 254 nm at 10 min. After liquid-liquid extraction, sample analyses were performed on a C(18) column by gradient elution; the mobile phase consisted of acetonitrile and phosphate buffer (0.014 m, pH 2.4). Owing to the limited amount of rabbit TCF available, a cross-validation of a proxy matrix was evaluated. The relative standard deviation of the between- and within-batch precision of both compounds was less than 5.1%; the relative error of the between- and within-batch accuracy was less than 7.3%. The recoveries of both compounds in serum and TCF were larger than 80%. This assay was successfully applied to simultaneously analyze piperacillin and tazobactam in rabbit serum and TCF samples.

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“…This fluid served as a bacterial growth medium. 10 Antibiotic assay HPLC methods were developed and validated to determine simultaneously the piperacillin/tazobactam (C. Li, D. Xuan, M. Ye, D. P. Nicolau and C. H. Nightingale, unpublished results) and ticarcillin/clavulanate concentrations, 11 respectively, in rabbit serum and TCF. The concentration ranges of the standard curves were 1-100 mg/L for piperacillin, tazobactam and ticarcillin, and 0.2-2 mg/L for clavulanate in rabbit serum and TCF.…”

Section: Rabbit Tissue Cage Modelmentioning

confidence: 99%

Pharmacodynamic study of -lactams alone and in combination with -lactamase inhibitors against Pseudomonas aeruginosa possessing an inducible -lactamase

Nicolau²,

Lister³

et al. 2004

Journal of Antimicrobial Chemotherapy

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Simultaneous determination of ticarcillin and clavulanate in rabbit serum and tissue cage fluid by liquid chromatography

Cited by 10 publications

References 24 publications

Determination of clavulanic acid in calf plasma by liquid chromatography tandem mass spectrometry

Determination of clavulanic acid in calf plasma by liquid chromatography tandem mass spectrometry

Simultaneous analysis of piperacillin and tazobactam in rabbits: application to pharmacokinetic study

Pharmacodynamic study of -lactams alone and in combination with -lactamase inhibitors against Pseudomonas aeruginosa possessing an inducible -lactamase

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